Abstract
As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the present study, by utilizing live cells fluorescent microscopy and co-immunoprecipitation assays, the nuclear import of UL31 was characterized to be dependent on Ran-, importin α1- and transportin-1-mediated pathway. Therefore, these results will promote the understanding of UL31-mediated biological functions in HSV-1 infection cycle.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 31400150
Award Identifier / Grant number: 31200120
Funding statement: This work was supported by grants from the National Natural Science Foundation of China (31400150 and 31200120); the Pearl River S&T Nova Program of Guangzhou (2013J2200018); the Natural Science Foundation of Guangdong Province (2015A030313473 and S2013040016596); the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (2013LYM_0096); the Science and Technology Program of Guangzhou, China (201504291022514); the Scientific Research Projects in Colleges and universities of Guangzhou (1201430024); the Scientific Research Foundation for the Ph.D., Guangzhou Medical University (2014C02); the Training Program for Outstanding Young Teachers in Universities of Guangdong Province (YQ2015132); the Medical Scientific Research Foundation of Guangdong Province, China (B2012165); the Thousand Hundred Ten Projects of Guangzhou Medical University, Guangdong; the National Undergraduate Training Programs for Innovation and Entrepreneurship (201510570006); and the Students’ extracurricular scientific and technological activities in Guangzhou Medical University (2015A008, 2015A019 and 2015B020). We thank Drs. Yoshinari Yasuda, Haitao Guo, Nobuyuki Nukina, Yoshihiro Yoneda, Ben Margolis, Christopher F. Basler, Shih-Ming Huang, Bryce M. Paschal, Reinhard Depping, Yasushi Kawaguchi and Chunfu Zheng, for the generous gifts of pGEX-Ran-Q69L, DN kα1, DN kβ1, RFP-M9M/RFP-Bimax2, pcDNA-Flag-kα1 (importin α5), pCMV9-3×FLAG-importin β1, Flag-kα6 (importin α7), Flag-kα2 (importin α1), Flag-kα4 (importin α3), HSV-1 F strain, respectively.
Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (31400150 and 31200120); the Pearl River S&T Nova Program of Guangzhou (2013J2200018); the Natural Science Foundation of Guangdong Province (2015A030313473 and S2013040016596); the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (2013LYM_0096); the Science and Technology Program of Guangzhou, China (201504291022514); the Scientific Research Projects in Colleges and universities of Guangzhou (1201430024); the Scientific Research Foundation for the Ph.D., Guangzhou Medical University (2014C02); the Training Program for Outstanding Young Teachers in Universities of Guangdong Province (YQ2015132); the Medical Scientific Research Foundation of Guangdong Province, China (B2012165); the Thousand Hundred Ten Projects of Guangzhou Medical University, Guangdong; the National Undergraduate Training Programs for Innovation and Entrepreneurship (201510570006); and the Students’ extracurricular scientific and technological activities in Guangzhou Medical University (2015A008, 2015A019 and 2015B020). We thank Drs. Yoshinari Yasuda, Haitao Guo, Nobuyuki Nukina, Yoshihiro Yoneda, Ben Margolis, Christopher F. Basler, Shih-Ming Huang, Bryce M. Paschal, Reinhard Depping, Yasushi Kawaguchi and Chunfu Zheng, for the generous gifts of pGEX-Ran-Q69L, DN kα1, DN kβ1, RFP-M9M/RFP-Bimax2, pcDNA-Flag-kα1 (importin α5), pCMV9-3×FLAG-importin β1, Flag-kα6 (importin α7), Flag-kα2 (importin α1), Flag-kα4 (importin α3), HSV-1 F strain, respectively.
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