Abstract
The concentration of neuron-specific enolase (NSE) in serum and cerebrospinal fluid (CSF) has been used as a biomarker in some cancers and, more recently, in neurodegenerative diseases. Pre-analytical conditions are very important for the quality of returned results. In this study, we evaluated the effects of storage conditions (temperature and duration of storage) and hemolysis on the concentration of NSE in serum and CSF. Our results demonstrate that samples for NSE measurement may be stored at −80°C for no more than 6 months in the case of CSF and 9months in the case of serum samples. Even invisible hemolysis may increase NSE levels in samples. Consequently, an index of hemolysis should be determined before deciding whether or not to perform NSE measurement.
References
1. Cooper EH. Neuron-specific enolase. Int J Biol Markers 1994; 9:205–10.10.1177/172460089400900401Search in Google Scholar
2. Gerbitz KD, Summer J, Schumacher I, Arnold H, Kraft A, Mross K. Enolase isoenzymes as tumour markers. J Clin Chem Clin Biochem 1986; 24:1009–16.10.1515/cclm.1986.24.12.1009Search in Google Scholar
3. Niklinski J, Furman M. Clinical tumour markers in lung cancer. Eur J Cancer Prev 1995; 4:129–38.10.1097/00008469-199504000-00002Search in Google Scholar
4. Riley RD, Heney D, Jones DR, Sutton AJ, Lambert PC, Abrams KR, et al. A systematic review of molecular and biological tumor markers in neuroblastoma. Clin Cancer Res 2004; 10:4–12.10.1158/1078-0432.CCR-1051-2Search in Google Scholar
5. Aksamit AJ, Preissner CM, Homburger HA. Quantitation of 14-3-3 and neuron-specific enolase proteins in CSF in Creutzfeldt-Jakob disease. Neurology 2001; 57:728–30.10.1212/WNL.57.4.728Search in Google Scholar
6. Verbeek MM, De Jong D, Kremer HP. Brain-specific proteins in CSF for the diagnosis of neurodegenerative diseases. Ann Clin Biochem 2003; 40:25–40.10.1258/000456303321016141Search in Google Scholar
7. Zuber E, Rosso L, Darbouret B, Socquet F, Mathis G, Flandrois JP. A descriptive model for the kinetics of a homogeneous fluorometric immunoassay. J Immunoassay 1997; 18:21–47.10.1080/01971529708005803Search in Google Scholar
8. Bissé E, Abraham EC. New less temperature-sensitive microchromatographic method for separation and quantitation of glycosylated hemoglobins using a non-cyanide buffer system. J Chromatogr 1985; 344:81–91.10.1016/S0378-4347(00)82009-5Search in Google Scholar
9. Beaudeux JL, Léger P, Dequen L, Gandjbakhch I, Coriat P, Foglietti MJ. Influence of hemolysis on the measurement of S-100β protein and neuron-specific enolase plasma concentrations during coronary artery bypass grafting. Clin Chem 2000; 46:989–90.10.1093/clinchem/46.7.989Search in Google Scholar
10. Mercer DW, Virji MA, Barry GE, Piper ML. New solid-phase enzyme immunoassay of neuron-specific enolase in serum: effect of storage temperature, lipemia, icterus, and hemolysis. Clin Chem 1990; 36:1519.10.1093/clinchem/36.8.1519Search in Google Scholar
©2005 by Walter de Gruyter Berlin New York