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Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme

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Abstract

In order to investigate the antioxidant properties of the polysaccharides from the brown alga Sargassum fusiforme, the crude polysaccharides from S. fusiforme (SFPS) were extracted in hot water, and the lipid peroxidation inhibition assay exhibited that SFPS possessed a potential antioxidant activity. Hence, two purely polymeric fractions, SFPS-1 and SFPS-2 were isolated by the column of DEAE (2-diethylaminoethanol)-Sepharose Fast Flow, with their molecular weights of 51.4 and 30.3 kDa determined by high performance gel permeation chromatography (HPGPC). They were preliminarily characterized using chemical analysis in combination of infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies and found to contain large amounts of uronic acids and β-glycosidical linkages. The antioxidant activities of these two SFPS fractions were evaluated using superoxide and hydroxyl radical-scavenging assays. The results show that the antioxidant ability of SFPS-2 was higher than that of SFPS-1, probably correlating with the molecular weight and uronic acid content.

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References

  • Bae, S.W., Suh, H.J., 2007. Antioxidant activities of five different mulberry cultivars in Korea. LWT-Food Science and Technology, 40(6):955–962. [doi:10.1016/j.lwt.2006. 06.007]

    Article  CAS  Google Scholar 

  • Barker, S.A., Bourne, E.J., Stacey, M., Whiffen, D.H., 1954. Infra-redspectra of carbohydrates. Part I. Some derivatives of D-glucopyranose. J. Chem. Soc., 1954:171–176. [doi:10.1039/JR9540000171]

    Article  Google Scholar 

  • Bitter, T., Muir, H.M., 1962. A modified uronic acid carbazole reaction. Anal. Biochem., 4(4):330–334. [doi:10.1016/0003-2697(62)90095-7]

    Article  PubMed  CAS  Google Scholar 

  • Butterfield, D.A., Castenga, A., Pocernich, C.B., Drake, J., Scapagnini, G., Calabrese, V., 2002. Nutritional approaches to combat oxidative stress in Alzheimer’s diseases. J. Nutr. Biochem., 13(8):444–461. [doi:10.1016/S0955-2863(02)00205-X]

    Article  PubMed  CAS  Google Scholar 

  • Chen, H., Zhang, M., Xie, B., 2004. Quantification of uronic acids in tea polysaccharide conjugates and their antioxidant properties. J. Agric. Food Chem., 52(11):3333–3336. [doi:10.1021/jf0349679]

    Article  PubMed  CAS  Google Scholar 

  • Chiarini, L., Cescutti, P., Drigo, L., Impallomeni, G., Herasimenka, Y., Bevivino, A., Dalmastri, C., Tabacchioni, S., Manno, G., Zanetti, F., Rizzo, R., 2004. Exopolysaccharides produced by Burkholderia cenocepacia recA lineages IIIA and IIIB. J. Cystic Fibr., 3(3):165–172. [doi:10.1016/j.jcf.2004.04.004]

    Article  CAS  Google Scholar 

  • Cozzolino, R., Malvagna, P., Spina, E., Giori, A., Fuzzati, N., Anelli, A., Garozzo, D., Impallomeni, G., 2006. Structural analysis of the polysaccharides from Echinacea angustifolia radix. Carbohydr. Polym., 65(3):263–272. [doi:10.1016/j.carbpol.2006.01.012]

    Article  CAS  Google Scholar 

  • Crowell, E.P., Burnett, B.B., 1967. Determination of the carbohydrate composition of wood pulps by gas chromatography of the alditol acetates. Anal. Chem., 39(1):121–124. [doi:10.1021/ac60245a006]

    Article  CAS  Google Scholar 

  • Dawczynski, C., Schubert, R., Jahreis, G., 2007. Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chem., 103(3):891–899. [doi:10.1016/j.foodchem. 2006.09.041]

    Article  CAS  Google Scholar 

  • Diplock, A.T., Charleux, J.L., Crozier-Willi, G., Kok, F.J., Rice-Evans, C., Robefroid, M., 1998. Functional food science and defense against reactive oxidative species. Br. J. Nutr., 80(1):S77–S112. [doi:10.1079/BJN19980106]

    Article  PubMed  CAS  Google Scholar 

  • Duan, X.J., Zhang, W.W., Li, X.M., Wang, B.G., 2006. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chem., 95(1):37–43. [doi:10.1016/j.foodchem.2004. 12.015]

    Article  CAS  Google Scholar 

  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28(3):350–356. [doi:10.1021/ac60111a017]

    Article  CAS  Google Scholar 

  • Halliwell, B., Aruoma, O.I., 1991. DNA damage by oxygen derived species. Its mechanism and measurement in mammalian systems. FEBS Lett., 281(1–2):9–19. [doi: 10.1016/0014-5793(91)80347-6]

    Article  PubMed  CAS  Google Scholar 

  • Halliwell, B., Gutteridge, J.M.C., Arurma, O.I., 1987. The deoxyribose method: a simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem., 165(1):215–219. [doi:10.1016/0003-2697(87)90222-3]

    Article  PubMed  CAS  Google Scholar 

  • Haroun-Bouhedja, F., Ellouali, M., Sinquin, C., Boisson-Vidal, C., 2000. Relationship between sulfate group and biological activities of fucans. Thromb. Res., 100(5):453–459. [doi:10.1016/S0049-3848(00)00338-8]

    Article  PubMed  CAS  Google Scholar 

  • He, S., Lu, Y.B., Wu, B., Pan, Y.J., 2007. Isolation and purification of antioxidative isomeric polyphenols from the roots of Parthenocissus laetevirens by ounter-current chromatograph. J. Chromatogr. A, 1151(1–2):175–179. [doi:10.1016/j.chroma.2007.02.102]

    Article  PubMed  CAS  Google Scholar 

  • Huang, X.X., Zhou, H.Q., Zhang, H., 2006. The effect of Sargassum fusiforme polysaccharide extracts on vibriosis resistance and immune activity of the shrimp. Fenneropenaeus chinensis. Fish Shellfish Immun., 20(5):750–757. [doi:10.1016/j.fsi.2005.09.008]

    Article  CAS  Google Scholar 

  • Im, S.A., Oh, S.T., Song, S., Kim, M.R., Kim, D.S., Woo, S.S., Jo, J.H., Park, Y.I., Lee, C.K., 2005. Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity. Int. Immunopharmacol., 5(2):271–279. [doi:10.1016/j.intimp. 2004.09.031]

    Article  PubMed  CAS  Google Scholar 

  • Ji, Y.B., Wang, C., Wu, T., Ji, C.F., 2007. Effect of Sargassum fusiforme polysaccharides on the complex mobility of erythrocytes in tumor-bearing organisms using high performance capillary electrophoresis. Chin. J. Chromatogr., 25(3):322–325. [doi:10.1016/S1872-2059(07)60011-X]

    Article  CAS  Google Scholar 

  • Juntachote, T., Berghofer, E., 2005. Antioxidative properties and stability of ethanolic extracts of Holy basil and Galangal. Food Chem., 92(2):193–202. [doi:10.1016/j.foodchem.2004.04.044]

    Article  CAS  Google Scholar 

  • Kuda, T., Tsunekawa, M., Goto, H., Araki, Y., 2005. Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. J. Food Compos. Anal., 18(7):625–633. [doi:10.1016/j.jfca.2004.06.015]

    Article  CAS  Google Scholar 

  • Lee, J.C., Kim, H.R., Kim, J., Jang, Y.S., 2002. Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. saboten. J. Agric. Food Chem., 50(22):6490–6496. [doi:10.1021/jf020388c]

    Article  PubMed  CAS  Google Scholar 

  • Leung, M.Y.K., Liu, C., Koon, J.C.M., Fung, K.P., 2006. Polysaccharide biological response modifiers. Immunol. Lett., 105(2):101–114. [doi:10.1016/j.imlet.2006.01.009]

    Article  PubMed  CAS  Google Scholar 

  • Liu, F., Ooi, V.E., Chang, S.T., 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci., 60(10):763–771. [doi:10.1016/S0024-3205(97)00004-0]

    Article  PubMed  CAS  Google Scholar 

  • Mao, W.J., Li, B.F., Gu, Q.Q., Fang, Y.C., Xing, H.T., 2004. Preliminary studies on the chemical characterization and antihyperlipidemic activity of polysaccharide from the brown alga Sargassum fusiforme. Hydrobiologia, 512(1–3):263–266. [doi:10.1023/B:HYDR.0000020335.46431.ad]

    Article  CAS  Google Scholar 

  • Park, P.J., Shahidi, F., Jeon, Y.J., 2004. Antioxidant activities of enzymatic extracts from and edible seaweed Sargassum horneri using ESR spectroscopy. J. Food Lipids, 11(1):15–27. [doi:10.1111/j.1745-4522.2004.tb00257.x]

    Article  Google Scholar 

  • Peterszegi, G., Robert, A.M., Robert, L., 2003. Protection by L-fucose and fucose-rich polysaccharides against ROS-produced cell death in presence of ascorbate. Biomed. Pharmacother., 57(3–4):130–133. [doi:10.1016/S0753-3322(03)00019-2]

    Article  PubMed  CAS  Google Scholar 

  • Rollet-Labelle, E., Grange, M.J., Elbim, C., Marquetty, C., Gougerot-Pocidalo, M.A., Pasquier, C., 1998. Hydroxyl radical as a potential intracellular mediator of polymorphonuclear neutrophil apoptosis. Free Radic. Biol. Med., 24(4):563–572. [doi:10.1016/S0891-5849(97)00292-X]

    Article  PubMed  CAS  Google Scholar 

  • Simas, F.F., Maurer-Menestrina, J., Reis, R.A., Sassaki, G.L., Iacomini, M., Gorin, P.A.G., 2006. Structure of the fucose-containing acidic heteroxylan from the gum exudate of Syagrus romanzoffiana (Queen palm). Carbohydr. Polym., 63(1):30–39. [doi:10.1016/j.carbpol.2005.06.015]

    Article  CAS  Google Scholar 

  • Singthong, J., Cui, S.W., Ningsanond, S., Goff, H.D., 2004. Structural characterization, degree of esterification and some gelling properties of Krueo Ma Noy (Cissampelos pareira) pectin. Carbohydr. Polym., 58(4):391–400. [doi:10.1016/j.carbpol.2004.07.018]

    Article  CAS  Google Scholar 

  • Tseng, Y.H., Yang, J.H., Mau, J.L., 2008. Antioxidant properties of polysaccharides from Ganoderma tsugae. Food Chem., 107(2):732–738.

    Article  CAS  Google Scholar 

  • Wang, Y., Zhang, M., Ruan, D., Shashkov, A.S., Kilcoyne, M., Savage, A.V., Zhang, L., 2004. Chemical components and molecular mass of six polysaccharides isolated from the sclerotium of Poria cocos. Carbohydr. Res., 339(2): 327–334. [doi:10.1016/j.carres.2003.10.006]

    Article  PubMed  CAS  Google Scholar 

  • Wu, X.M., Ouyang, J.M., Deng, S.P., Cen, Y.Z., 2006. Inhibition of the crystal growth and aggregation of calcium oxalate by algae sulfated polysaccharide in-vitro. Chinese Chem. Lett., 17(1):97–100.

    CAS  Google Scholar 

  • Wu, Y., Hu, N., Pan, Y., Zhou, L., Zhou, X., 2007. Isolation and characterization of a mannoglucan from edible Cordyceps sinensis mycelium. Carbohydrate Res., 342(6): 870–875. [doi:10.1016/j.carres.2007.01.005]

    Article  CAS  Google Scholar 

  • Yan, X., Nagata, T., Fan, X., 1998. Antioxidative activities in some common seaweeds. Plant Food Hum. Nutr., 52(3): 253–262. [doi:10.1023/A:1008007014659]

    Article  CAS  Google Scholar 

  • Zhang, L., Lin, Y., 2008. Tannins from Canarium album with potent antioxidant activity. J. Zhejiang Univ. Sci. B, 9(5): 407–415. [doi:10.1631/jzus.B0820002]

    Article  PubMed  CAS  Google Scholar 

  • Zhang, M., Cui, S.W., Cheung, P.C.K., Wang, Q., 2007. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci. Tech., 18(1):4–19. [doi:10.1016/j.tifs.2006.07.013]

    Article  CAS  Google Scholar 

  • Zhang, Q.B., Li, N., Zhou, G.F., Lu, X.L., Xu, Z.H., Li, Z., 2003. In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice. Pharmacol. Res., 48(2):151–155. [doi:10.1016/ S1043-6618(03)00103-8]

    Article  PubMed  CAS  Google Scholar 

  • Zhao, T.T., Zhang, Q.B., Qi, H.M., Zhang, H., Niu, X.Z., Xu, Z.H., Li, Z., 2006. Degradation of porphyran from Porphyra haitanensis and the antioxidant activities of the degraded porphyrans with different molecular weight. Int. J. Biol. Macromol., 38(1):45–50. [doi:10.1016/j.ijbiomac. 2005.12.018]

    Article  PubMed  CAS  Google Scholar 

  • Zhu, W., Ooi, V.E.C., Chan, P.K.S., Ang, P.O. Jr., 2003. Isolation and characterization of a sulfated polysaccharide from the brown alga Sargassum patens and determination of its anti-herpes activity. Biochem. Cell Biol., 81(1): 25–33. [doi:10.1139/o02-169]

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Zhejiang University, Hangzhou, 310027, China

    Jing Zhou, Nan Hu, Ya-lin Wu, Yuan-jiang Pan & Cui-rong Sun

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  1. Jing Zhou
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  2. Nan Hu
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  3. Ya-lin Wu
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Correspondence to Cui-rong Sun.

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Project (No. 20772109) supported by the National Natural Science Foundation of China

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Zhou, J., Hu, N., Wu, Yl. et al. Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme . J. Zhejiang Univ. Sci. B 9, 721–727 (2008). https://doi.org/10.1631/jzus.B0820025

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  • DOI: https://doi.org/10.1631/jzus.B0820025

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