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DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters

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Abstract

Objective

To investigate the expression of death decoy receptor 3 (DcR3) and survivin in colorectal carcinoma.

Methods

Tumor and normal tissues were taken from a total of 100 colorectal carcinoma patients during surgery, and the expression of DcR3 and survivin was examined by immunohistochemistry, Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR) analyses.

Results

RT-PCR showed that the expression levels of DcR3 mRNA (0.846±0.242, P<0.01) and survivin mRNA (0.7835±0.2392, P<0.01) in colorectal cancer tissues were significantly higher than those in adjacent normal tissues. Western blotting showed that the expression levels of DcR3 protein (0.795±0.261, P<0.01) and survivin protein (0.6765±0.1351, P<0.01) in tumor tissues were significantly higher than those in non-cancer tissues. The immunohistochemical streptavidin-peroxidase (SP) method showed that the positive expression rates of DcR3 and survivin were 67.0% and 58.0% in colorectal cancer tissues, and 18.0% and 3.0% in non-cancerous colorectal tissues (P<0.05), respectively. The positive correlations of DcR3 (P<0.01) and survivin (P<0.01) to the differentiation of colorectal carcinoma cells, lymph node metastasis, and pathological stage were observed. The expression of DcR3 and survivin was found to be positively correlated to clinicopathologic parameters of colorectal carcinoma.

Conclusion

The overexpressed DcR3 and survivin in colorectal cancer may contribute to the development of the cancer. The monitoring of these two proteins may be useful for the diagnosis, differentiation, metastasis, and determination of stages of colorectal carcinoma.

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References

  • Bai, C., Connolly, B., Metzker, M.L., Hilliard, C.A., Liu, X., Sandig, V., Soderman, A., Galloway, S.M., Liu, Q., Austin, C.P., Caskey, C.T., 2000. Overexpression of M68/DcR3 in human gastrointestinal tract tumors independent of gene amplification and its location in a four-gene cluster. Proc. Natl. Acad. Sci. USA, 97(3): 1230–1235. [doi:10.1073/pnas.97.3.1230]

    Article  PubMed  CAS  Google Scholar 

  • Chang, P.M., Chen, P.M., Hsieh, S.L., Tzeng, C.H., Liu, J.H., Chiou, T.J., Wang, W.S., Yen, C.C., Gau, J.P., Yang, M.H., 2008. Expression of a soluble decoy receptor 3 in patients with diffuse large B-cell lymphoma predicts clinical outcome. Int. J. Oncol., 33(3):549–554.

    PubMed  CAS  Google Scholar 

  • Chen, C., Zhang, C., Zhuang, G., Luo, H., Su, J., Yin, P., Wang, J., 2008. Decoy receptor 3 overexpression and immunologic tolerance in hepatocellular carcinoma (HCC) development. Cancer Invest., 26(10):965–974. [doi:10. 1080/07357900801975256]

    Article  PubMed  CAS  Google Scholar 

  • Chen, P.H., Yang, C.R., 2008. Decoy receptor 3 expression in AsPC-1 human pancreatic adenocarcinoma cells via the phosphatidylinositol 3-kinase-, Akt-, and NF-kappaB-dependent pathway. J. Immunol., 181(12):8441–8449.

    PubMed  CAS  Google Scholar 

  • Connor, J.P., Felder, M., 2008. Ascites from epithelial ovarian cancer contain high levels of functional decoy receptor 3 (DcR3) and is associated with platinum resistance. Gynecol. Oncol., 111(2):330–335. [doi:10.1016/j.ygyno.2008. 07.012]

    Article  PubMed  CAS  Google Scholar 

  • Elnemr, A., Ohta, T., Yachie, A., Kayahara, M., Kitagawa, H., Fujimura, T., Ninomiya, I., Fushida, S., Nishimura, G.I., Shimizu, K., Miwa, K., 2001. Human pancreatic cancer cells disable function of Fas receptors at several levels in Fas signal transduction pathway. Int. J. Oncol., 18(2): 311–316.

    PubMed  CAS  Google Scholar 

  • Fayad, R., Brand, M.I., Stone, D., Keshavarzian, A., Qian, L., 2006. Apoptosis resistance in ulcerative colitis: high expression of decoy receptors by lamina propria T cells. Eur. J. Immunol., 36(8):2215–2222. [doi:10.1002/eji.2005 35477]

    Article  PubMed  CAS  Google Scholar 

  • Gill, R.M., Coleman, N.M., Hunt, J.S., 2007. Differential cellular expression of LIGHT and its receptors in early gestation human placentas. J. Reprod. Immunol., 74(1–2): 1–6. [doi:10.1016/j.jri.2006.08.083]

    Article  PubMed  CAS  Google Scholar 

  • Hayashi, S., Miura, Y., Nishiyama, T., Mitani, M., Tateishi, K., Sakai, Y., Hashiramoto, A., Kurosaka, M., Shiozawa, S., Doita, M., 2007. Decoy receptor 3 expressed in rheumatoid synovial fibroblasts protects the cells against Fas-induced apoptosis. Arthritis Rheum., 56(4):1067–1075. [doi:10.1002/art.22494]

    Article  PubMed  CAS  Google Scholar 

  • Inoue, Y., Morinaga, A., Takizawa, F., Saito, T., Endo, M., Haruta, C., Nakai, T., Moritomo, T., Nakanishi, T., 2008. Molecular cloning and preliminary expression analysis of banded dogfish (Triakis scyllia) TNF decoy receptor 3 (TNFRSF6B). Fish Shellfish Immunol., 24(3):360–365. [doi:10.1016/j.fsi.2007.08.005]

    Article  PubMed  CAS  Google Scholar 

  • Kim, S., Fotiadu, A., Kotoula, V., 2005. Increased expression of soluble decoy receptor 3 in acutely inflamed intestinal epithelia. Clin. Immunol., 115(3):286–294. [doi:10.1016/j.clim.2005.02.014]

    Article  PubMed  CAS  Google Scholar 

  • Lee, C.S., Hu, C.Y., Tsai, H.F., Wu, C.S., Hsieh, S.L., Liu, L.C., Hsu, P.N., 2008. Elevated serum decoy receptor 3 with enhanced T cell activation in systemic lupus erythematosus. Clin. Exp. Immunol., 151(3):383–390.

    Article  PubMed  CAS  Google Scholar 

  • Li, D.L., Yu, Q.Z., Feng, X.G., Li, M., Lu, W., 2005. The measures and experiences on actualizing “The Diagnosis and Treatment Criterion for Common Cancer in China” in Shanghai. Bull. Chin. Cancer, 14(1):3–7 (in Chinese).

    Google Scholar 

  • Macher-Goeppinger, S., Aulmann, S., Wagener, N., Funke, B., Tagscherer, K.E., Haferkamp, A., Hohenfellner, M., Kim, S., Autschbach, F., Schirmacher, P., Roth, W., 2008. Decoy receptor 3 is a prognostic factor in renal cell cancer. Neoplasia, 10(10):1049–1056.

    PubMed  CAS  Google Scholar 

  • Mild, G., Bachmann, F., Boulay, J.L., Glatz, K., Laffer, U., Lowy, A., Metzger, U., Reuter, J., Terracciano, L., Herrmann, R., Rochlitz, C., 2002. DcR3 locus is a predictive marker for 5-fluorouracil-based adjuvant chemotherapy in colorectal cancer. Int. J. Cancer, 102(3):254–257. [doi:10.1002/ijc.10711]

    Article  PubMed  CAS  Google Scholar 

  • Pitti, R.M., Marsters, S.A., Lawrence, D.A., Roy, M., Kischkel, F.C., Dowd, P., Huang, A., Donahue, C.J., Sherwood, S.W., Baldwin, D.T., et al., 1998. Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature, 396(17):699–703. [doi:10.1038/25387]

    PubMed  CAS  Google Scholar 

  • Roth, W., Isenmann, S., Nakamura, M., Platten, M., Wick, W., Kleihues, P., Bahr, M., Ohgaki, H., Ashkenazi, A., Weller, M., 2001. Soluble decoy receptor 3 is expressed by malignant gliomas and suppresses CD95 ligand-induced apoptosis and chemotaxis. Cancer Res., 61(6):2759–2765.

    PubMed  CAS  Google Scholar 

  • Sarela, A.I., Macadam, R.C., Farmery, S.M., Markham, A.F., Guillou, P.J., 2000. Expression of the antiapoptosis gene, survivin, predicts death from recurrent colorectal carcinoma. Gut, 46(5):645–650. [doi:10.1136/gut.46.5.645]

    Article  PubMed  CAS  Google Scholar 

  • Sarela, A.I., Verbeke, C.S., Ramsdale, J., Davies, C.L., Markham, A.F., Guillou, P.J., 2002. Expression of survivin, a novel inhibitor of apoptosis and cell cycle regulatory protein, in pancreatic adenocarcinoma. Br. J. Cancer, 86(6):886–892. [doi:10.1038/sj.bjc.6600133]

    Article  PubMed  CAS  Google Scholar 

  • Shen, H.W., Wu, Y.L., Peng, S.Y., 2003. Overexpression and genomic amplification of decoy receptor 3 in hepatocellular carcinoma and significance thereof. Natl. Med. J. China, 83(9):744–747 (in Chinese).

    CAS  Google Scholar 

  • Takahama, Y., Yamada, Y., Emoto, K., Fujimoto, H., Takayama, T., Ueno, M., Uchida, H., Hirao, S., Mizuno, T., Nakajima, Y., 2002. The prognostic significance of overexpression of the decoy receptor for Fas ligand (DcR3) in patients with gastric carcinomas. Gastric. Cancer, 5(2):61–68. [doi:10.1007/s101200200011]

    Article  PubMed  CAS  Google Scholar 

  • Tamm, I., Wang, Y., Sausville, E., Scudiero, D.A., Vigna, N., Oltersdorf, T., Reed, J.C., 1998. IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs. Cancer Res., 58(23):5315–5320.

    PubMed  CAS  Google Scholar 

  • Tanaka, K., Iwamoto, S., Gon, G., Nohara, T., Iwamoto, M., Tanigawa, N., 2000. Expression of survivin and its relationship to loss of apoptosis in breast carcinomas. Clin. Cancer Res., 6(1):127–134.

    PubMed  CAS  Google Scholar 

  • Wu, Y., Han, B., Sheng, H., Lin, M., Moore, P.A., Zhang, J., Wu, J., 2003. Clinical significance of detecting elevated serum DcR3/TR6/M68 in malignant tumor patients. Int. J. Cancer, 105(5):724–732. [doi:10.1002/ijc.11138]

    Article  PubMed  CAS  Google Scholar 

  • Xia, C., Xu, Z., Yuan, X., Uematsu, K., You, L., Li, K., Li, L., McCormick, F., Jablons, D.M., 2002. Induction of apoptosis in mesothelioma cells by antisurvivin oligonucleotides. Mol. Cancer Ther., 1(9):687–694.

    PubMed  CAS  Google Scholar 

  • Zhu, X.D., Lin, G.J., Qian, L.P., Chen, Z.Q., 2003. Expression of survivin in human gastric carcinoma and gastric carcinoma model of rats. World J. Gastroenterol., 9(7): 1435–1438.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medical Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524001, China

    Qi-lian Liang, Bi-rong Wang & Guo-hong Li

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  1. Qi-lian Liang
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  2. Bi-rong Wang
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Correspondence to Qi-lian Liang.

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Liang, Ql., Wang, Br. & Li, Gh. DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters. J. Zhejiang Univ. Sci. B 10, 675–682 (2009). https://doi.org/10.1631/jzus.B0920077

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  • DOI: https://doi.org/10.1631/jzus.B0920077

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