Abstract
The O subfamily of forkhead box (FoxO) proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B (IGF-1/PI3K/PKB) signal pathway. The objective of the present study was to examine the expressions of three members of FoxO proteins, FoxO1, FoxO3a, and FoxO4 in the duodenum of Sprague-Dawley rats at different ages. The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner. At Day 21, there were no detectable localization and expression of FoxO4 in the duodenum, while, at Months 2 and 6, localization and expression of FoxO4 were distinct. In addition, FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats, but was not detectable in the same area in 6-month-old rats. Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2- and 6-month marks, it was still not detectable at Day 21. Besides, FoxO1 was not detectable in all parts and stages. Taken together, our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.
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Bao, S., Ouyang, G., Bai, X., Huang, Z., Ma, C., Liu, M., Shao, R., Anderson, R.M., Rich, J.N., Wang, X.F., 2004. Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway. Cancer Cell, 5(4):329–339. [doi:10.1016/S1535-6108(04)00081-9]
Brunet, A., Bonni, A., Zigmond, M.J., Lin, M.Z., Juo, P., Hu, L.S., Anderson, M.J., Arden, K.C., Blenis, J., Greenberg, M.E., 1999. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell, 96(6):857–868. [doi:10.1016/S0092-8674(00)80595-4]
Burgering, B.M., Kops, G.J., 2002. Cell cycle and death control: long live forkheads. Trends Biochem. Sci., 27(7): 352–360. [doi:10.1016/S0968-0004(02)02113-8]
Ding, W., Wang, W., Zhou, B., Zhang, W., Huang, P., Shi, F., Taya, K., 2010. Formation of primordial follicles and immunolocalization of PTEN, PKB and FOXO3A proteins in the ovaries of fetal and neonatal pigs. J. Reprod. Dev., 56(1):162–168. [doi:10.1262/jrd.09-094H]
Furuyama, T., Yamashita, H., Kitayama, K., Higami, Y., Shimokawa, I., Mori, N., 2002. Effects of aging and caloric restriction on the gene expression of FoxO1, 3, and 4 (FKHR, FKHRL1, and AFX) in the rat skeletal muscles. Microsc. Res. Tech., 59(4):331–334. [doi:10.1002/jemt.10213]
Gao, J., Wang, S., Xing, S., 1995. Histological and histochemical observation on the development and differentiation of the mouse duodenum. Acta Anat. Sin., 26(5): 426–431.
Hoekman, M.F., Jacobs, F.M., Smidt, M.P., Burbach, J.P., 2006. Spatial and temporal expression of FoxO transcription factors in the developing and adult murine brain. Gene Expr. Patterns, 6(2):134–140. [doi:10.1016/j.modgep.2005.07.003]
Liu, Y., Wang, Y., Shan, T., 2008. The tissue-specific and developmental expression patterns of the forkhead transcription factor FoxO1 gene in pigs. J. Anim. Feed Sci., 17(2):182–190.
Meng, C., Shi, F., Zhou, Z., Huang, R., Liu, G., Watanabe, G., Taya, K., 2007. Cellular localization of inhibin α-subunit, PKB/Akt and FoxO3a proteins in the ovaries of minipigs. J. Reprod. Dev., 53(2):229–236. [doi:10.1262/jrd.18078]
Roubenoff, R., Hughes, V.A., 2000. Sarcopenia: current concepts. J. Gerontol. A Biol. Sci. Med. Sci., 55(12): M716–M724. [doi:10.1093/gerona/55.12.M716]
Saito, Y., Gopalan, B., Mhashilkar, A.M., Roth, J.A., Chada, S., Zumstein, L., Ramesh, R., 2003. Adenovirus-mediated PTEN treatment combined with caffeine produces a synergistic therapeutic effect in colorectal cancer cells. Cancer Gene Ther., 10(11):803–813. [doi:10.1038/sj.cgt.7700644]
Sengupta, A., Molkentin, J.D., Yutzey, K.E., 2009. FoxO transcription factors promote autophagy in cardiomyocytes. J. Biol. Chem., 284(41):28319–28331. [doi:10.1074/jbc.M109.024406]
Shi, F., Stewart, R.L.Jr., Perez, E., Chen, J.Y., LaPolt, P.S., 2004. Cell-specific expression and regulation of soluble guanylyl cyclase α1 and β1 subunits in the rat ovary. Biol. Reprod., 70(6):1552–1561. [doi:10.1095/biolreprod.103.025510]
Skurk, C., Maatz, H., Kim, H.S., Yang, J., Abid, M.R., Aird, W.C., Walsh, K., 2004. The Akt-regulated forkhead transcription factor FOXO3a controls endothelial cell viability through modulation of the caspase-8 inhibitor FLIP. J. Biol. Chem., 279(2):1513–1525. [doi:10.1074/jbc.M304736200]
Tomita, M., Shimokawa, I., Higami, Y., Yanagihara-Outa, K., Kawahara, T., Tanaka, K., Ikeda, T., Shindo, H., 2001. Modulation by dietary restriction in gene expression related to insulin-like growth factor-1 in rat muscle. Aging, 13(4):261–262.
van der Heide, L.P., Hoekman, M.F., Smidt, M.P., 2004. The ins and outs of FoxO shuttling: mechanisms of FoxO translocation and transcriptional regulation. Biochem. J., 380(2):297–309. [doi:10.1042/BJ20040167]
Zhang, W., Wei, Q., Wang, Z., Ding, W., Wang, W., Shi, F., 2011. Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats. J. Zhejiang Univ.-Sci. B, 12(1):55–64. [doi:10.1631/jzus.B1000174]
Zhou, Z., Wang, T., Pan, L., Huang, R., Shi, F., 2007. FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs. J. Zhejiang Univ.-Sci. B, 8(1):39–44. [doi:10.1631/jzus.2007.B0039]
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Project supported by the National Natural Science Foundation of China (No. 30771553) and the Basic Research Foundation for Science and Technology of Nanjing Agricultural University, China
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Huang, P., Zhou, Zq., Huang, Rh. et al. Age-dependent expression of forkhead box O proteins in the duodenum of rats. J. Zhejiang Univ. Sci. B 12, 730–735 (2011). https://doi.org/10.1631/jzus.B1000298
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DOI: https://doi.org/10.1631/jzus.B1000298