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Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation

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Abstract

To evaluate the effects of sperm with different parameters and sources on the outcomes of intracytoplasmic sperm injection (ICSI), 1972 ICSI cycles were analyzed retrospectively. Groups 1 to 5 were composed of cycles using ejaculated sperm and were grouped according to sperm quantity, quality, and morphology into normal (288 cycles), or mild (329 cycles), moderate (522 cycles), severe (332 cycles), and extremely severe (171 cycles) oligozoospermia and/or asthenozoospermia and/or teratozoospermia (OAT) groups. Group 6 was composed of 250 cycles using testicular or epididymal sperm, and Group 7 consisted of 80 cycles using frozen-thawed sperm. We found that fertilization rates were gradually reduced from Groups 1 to 6, and reached statistical difference in Groups 5 and 6 (P<0.05). The high-quality embryo rate was higher in Group 1 than in Groups 2, 3, 5, 6, and 7 (P<0.05). No statistical differences were observed in the rates of embryo cleavage, clinical pregnancy, miscarriage, live-birth, premature birth, low birth weight, weeks of premature birth, average birth weight, or sex ratio for all seven groups (P>0.05). A total of nine cases of malformation were observed, with a malformation rate of 1.25% (9/719). In conclusion, different sperm sources and parameters can affect ICSI outcomes before embryo implantation. A full assessment of offspring malformation will require further study using a larger sample size.

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  1. Department of Reproductive Endocrinology, Key Laboratory of Reproductive Genetics, Ministry of Education, and Key Laboratory of Women’s Reproductive Health of Zhejiang Province, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China

    Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang & Fan Jin

  2. Department of Reproductive Health, Shaoxing Women and Children Hospital, Shaoxing, 312000, China

    Yue-hong Lu

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  1. Yue-hong Lu
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Correspondence to Fan Jin.

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The two authors contributed equally to this work

Project supported by the National Natural Science Foundation of China (No. 81070532), the National Basic Research Program (973) of China (Nos. 2012CB944901 and 2007CB948104), and the Zhejiang Provincial Natural Science Foundation of China (No. Z207021)

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Lu, Yh., Gao, Hj., Li, Bj. et al. Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation. J. Zhejiang Univ. Sci. B 13, 1–10 (2012). https://doi.org/10.1631/jzus.B1100216

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