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Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers

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Abstract

A total of 50 does were used to determine selected hematological and biochemical parameters with special references to oxidative stress markers, acute phase protein profiles, and proinflammatory cytokines in healthy and gangrenous mastitis affected does. Animals were divided into two equal groups represented as clinically healthy (control) and diseased groups, respectively. The bacteriological examination of milk samples from diseased does revealed many types of bacterial infection. The isolated bacteria were Staphylococcus aureus (N=23/25), Escherichia coli (N=11/25), and Clostridium perfringens (N=4/25). There was a significant increase in the levels of β-hydroxybutyrate, non-esterified free fatty acids, triglyceride, low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase, and alanine aminotransferase and a significant reduction in the levels of glucose, cholesterol, and high density lipoprotein cholesterol (HDL-C) in does with gangrenous mastitis compared to healthy does. Moreover, there was a significant increase in the levels of malondialdehyde and uric acid with a significant decrease in the levels of reduced glutathione, super oxide dismutase, and catalase in does with gangrenous mastitis compared to healthy does. In addition, there was a significant increase in the haptoglobin, serum amyloid A, fibrinogen, interleukin 6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) in does with gangrenous mastitis compared to healthy ones. Conclusively, oxidative stress biomarkers, acute phase proteins, and proinflammatory cytokines play an essential task as biomarkers for gangrenous mastitis in does. Mastitis may be considered as one of the ketotic stressors in does after parturition.

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Authors and Affiliations

  1. Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, P.O. Box 400, Al-Ahsa, 31982, Saudi Arabia

    Wael M. El-Deeb

  2. Department of Veterinary Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

    Wael M. El-Deeb

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  1. Wael M. El-Deeb
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Correspondence to Wael M. El-Deeb.

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Project (No. 110031) supported by the Deanship of Scientific Research, King Faisal University, Saudi Arabia

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El-Deeb, W.M. Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers. J. Zhejiang Univ. Sci. B 14, 33–39 (2013). https://doi.org/10.1631/jzus.B1200123

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  • DOI: https://doi.org/10.1631/jzus.B1200123

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