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Isolation and Pharmacological Characterization of Fatty Acids from Saw Palmetto Extract

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Abstract

Saw palmetto extract (SPE) has been widely used for the treatment of lower urinary-tract symptoms secondary to benign prostatic hyperplasia. The mechanisms of pharmacological effects of SPE include the inhibition of 5a-reductase, anti-androgenic effects, anti-proliferative effects, and anti-inflammatory effects. Previously, we showed that SPE bound actively to a1-adrenergic, muscarinic and 1,4-dihydropyridine calcium channel (1,4-DHP) receptors in the prostate and bladder of rats, whereas its active constituents have not been fully clarified. The present investigation is aimed to identify the main active components contained in hexane and diethyl ether extracts of SPE with the use of column chromatography and preparative HPLC. Based on the binding activity with a1-adrenergic, muscarinic, and 1,4-DHP receptors, both isolated oleic and lauric acids were deduced to be active components. Authentic samples of oleic and lauric acids also exhibited similar binding activities to these receptors as the fatty acids isolated from SPE, consistent with our findings. In addition, oleic and lauric acids inhibited 5α-reductase, possibly leading to therapeutic effects against benign prostatic hyperplasia and related lower urinary-tract symptoms.

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References

  1. R. M. Levin and A. K. Das, Urol. Res., 2000, 28, 201.

    Article  CAS  Google Scholar 

  2. H. Lepor, W. O. Williford, M. J. Barry, M. K. Brawer, C. M. Dixon, G. Gormley, C. Haakenson, M. Machi, P. Narayan, and R. J. Padley, N. Engl. J. Med., 1996, 335, 533.

    Article  CAS  Google Scholar 

  3. T. J. Wilt, A. Ishani, G. Stark, R. MacDonald, J. Lau, and C. Mulrow, J. A. M. A., 1998, 280, 1604.

    Article  CAS  Google Scholar 

  4. M. Suzuki, Y. Ito, T. Fujino, M. Abe, K. Umegaki, S. Onoue, H. Noguchi, and S. Yamada, Acta Pharmacol. Sin., 2009, 30, 27.

    Article  CAS  Google Scholar 

  5. C. Iehlé, S. Délos, O. Guirou, R. Tate, J. P. Raynaud, and P. M. Martin, J. Steroid Biochem. Mol. Biol., 1995, 54, 273.

    Article  Google Scholar 

  6. T. Liang and S. Liao, Biochem. J., 1992, 285, 557.

    Article  CAS  Google Scholar 

  7. M. Goepel, U. Hecker, S. Krege, H. Rübben, and M. C. Michel, Prostate, 1999, 38, 208.

    Article  CAS  Google Scholar 

  8. C. Sultan, A. Terraza, C. Devillier, E. Carilla, M. Briley, C. Loire, and B. Descomps, J. Steroid Biochem., 1984, 20, 515.

    Article  CAS  Google Scholar 

  9. L. S. Adams, N. P. Seeram, M. L. Hardy, C. Carpenter, and D. Heber, Evid. Based Complement. Alternat. Med., 2006, 3, 117.

    Article  Google Scholar 

  10. W. Breu, M. Hagenlocher, K. Redl, G. Tittel, F. Stadler, and H. Wagner, Arzneim. Forsch., 1992, 42, 547.

    CAS  Google Scholar 

  11. T. Oki, M. Suzuki, Y. Nishioka, A. Yasuda, K. Umegaki, and S. Yamada, J. Urol., 2005, 173, 1395.

    Article  Google Scholar 

  12. M. Suzuki, T. Oki, T. Sugiyama, K. Umegaki, S. Uchida, and S. Yamada, Urol., 2007, 69, 1216.

    Article  Google Scholar 

  13. T. Oki-Fujino, M. Suzuki, and S. Yamada, Jpn. J.complement Altern. Med., 2007, 4, 41.

    Google Scholar 

  14. F. J. Ehlert and L. P. Tran, J. Pharmacol. Exp. Ther., 1990, 255, 1148.

    PubMed  CAS  Google Scholar 

  15. S. Yamada, N. Ashizawa, H. Ushijima, K. Nakayama, E. Hayashi, and K. Honda, J. Pharmacol. Exp. Ther., 1987, 242, 326.

    PubMed  CAS  Google Scholar 

  16. S. Yamada, R. Kimura, Y. Harada, and K. Nakayama, J. Pharmacol. Exp. Ther., 1990, 252, 327.

    PubMed  CAS  Google Scholar 

  17. K. Mitamura, C. Ogasawara, A. Shiozawa, E. Terayama, and K. Shimada, Anal. Sci., 2005, 21, 1241.

    Article  CAS  Google Scholar 

  18. J. Liu, K. Kurashiki, K. Shimizu, and R. Kondo, Biol. Pharm. Bull., 2006, 29, 392.

    Article  Google Scholar 

  19. M. M. Bradford, Anal. Biochem., 1976, 72, 248.

    Article  CAS  Google Scholar 

  20. H. Weisser, S. Tunn, B. Behnke, and M. Krieg, Prostate, 1996, 28, 300.

    Article  CAS  Google Scholar 

  21. E. Neuzil and H. Cousse, Bull. Soc. Pharm. Bordeaux, 1993, 132, 121.

    CAS  Google Scholar 

  22. H. J. Niederprüm, H. U. Schweikert, and K. S. Zanker, Phytomedicine, 1994, 1, 127.

    Article  Google Scholar 

  23. M. J. Barry, J. A. M. A., 1997, 278, 2178.

    Article  CAS  Google Scholar 

  24. J. P. Raynaud, H. Cousse, and P. M. Martin, J. Steroid Biochem. Mol. Biol., 2002, 82, 233.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Departments of Pharmacokinetics and Pharmacodynamics, Pharmacognosy and Global Center of Excellence (COE) Program, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka, 422-8526, Japan

    Masayuki Abe, Yoshihiko Ito, Satomi Onoue, Hiroshi Noguchi & Shizuo Yamada

  2. Development Division Research Section, Q’SAI CO., LTD., 1-7-16 Kusagae, Chuo, Fukuoka, 810-8606, Japan

    Asahi Suzuki

Authors
  1. Masayuki Abe
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  2. Yoshihiko Ito
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  3. Asahi Suzuki
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  4. Satomi Onoue
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  5. Hiroshi Noguchi
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  6. Shizuo Yamada
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Corresponding author

Correspondence to Shizuo Yamada.

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Abe, M., Ito, Y., Suzuki, A. et al. Isolation and Pharmacological Characterization of Fatty Acids from Saw Palmetto Extract. ANAL. SCI. 25, 553–557 (2009). https://doi.org/10.2116/analsci.25.553

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  • DOI: https://doi.org/10.2116/analsci.25.553

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