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Paraoxonase 1 (PON1) and its relationship to lipid variables, age and gender in healthy volunteers

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Abstract

Recent studies implied that low-density lipoprotein (LDL) modified predominantly by oxidation or glycation, significantly contributes to the formation of atherosclerotic lesions. In contrast to oxidized LDL (ox-LDL), high-density lipoprotein (HDL) is able to prevent accumulation of ox-LDL in arterial walls. This antiatherogenic property of HDL is attributed in part to several enzymes associated with the lipoprotein, including HDL-associated paraoxonase 1 (PON1). In this study we analyzed PON1 arylesterase/paraoxonase activities in relation to serum lipid profile, gender and age in thirty clinically healthy Slovak volunteers. Our results showed that PON1 arylesterase and paraoxonase activities were lower in citrated plasma than in serum by 16.6% and 27.3%, respectively. Among serum lipoproteins, only HDL-cholesterol level showed significant positive correlation with PON1 arylesterase activity (p = 0.042). Likewise, we found a significant relationship between atherogenic index (AI = total cholesterol/HDL-cholesterol) and PON1 arylesterase activity (p = 0.023). No significant correlation could be demonstrated between PON1 paraoxonase activity and serum lipid profile, age or gender. Furthermore, it was found that PON1 paraoxonase/arylesterase activities were higher in women compared with both investigated activities in men, but these differences were not statistically significant. These results confirmed a positive correlation between HDL-cholesterol and PON1 arylesterase activity. Moreover, it was found out that PON1 paraoxonase activity is not influenced either by gender or by age. PON1 arylesterase activity was however affected by gender to a limited extent.

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Abbreviations

AI:

atherogenic index

PON1:

paraoxonase 1

HDL:

high-density lipoprotein

LDL:

low-density lipoprotein

TAG:

triacylglycerols

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Authors and Affiliations

  1. Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, SK-81372, Bratislava, Slovakia

    Katarína Sumegová & Zdeňka Ďuračková

  2. Division of Biochemical Clinical Laboratories, Hospital of the Ministry of Defence, Bratislava, Slovakia

    Pavel Blažíček

  3. Lipid Research Laboratory, Technion Faculty of Medicine and Rambam Medical Center, Haifa, Israel

    Bianca Fuhrman

  4. Department of Nuclear Physics and Biophysics, Division of Biomedical Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia

    Iveta Waczulíková

Authors
  1. Katarína Sumegová
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  2. Pavel Blažíček
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  3. Bianca Fuhrman
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  4. Iveta Waczulíková
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  5. Zdeňka Ďuračková
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Correspondence to Zdeňka Ďuračková.

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Sumegová, K., Blažíček, P., Fuhrman, B. et al. Paraoxonase 1 (PON1) and its relationship to lipid variables, age and gender in healthy volunteers. Biologia 61, 699–704 (2006). https://doi.org/10.2478/s11756-006-0143-4

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  • DOI: https://doi.org/10.2478/s11756-006-0143-4

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