Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Smilax china Root Extract in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Extract Preparation and Analysis
2.1.1. Preparation of FSC
2.1.2. Quantification of GABA Content
2.2. Animals and Experimental Design
2.2.1. Animals
2.2.2. Experimental Design for Alcohol Metabolism after Acute Ethanol Administration
2.2.3. Experimental Design
2.2.4. Alcohol and Acetaldehyde Concentration in Serum
2.2.5. Measurement of Liver Biomarkers in Serum
2.2.6. Histopathology
2.2.7. Total RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)
2.3. Acute Oral Toxicity Test
2.4. Statistical Analysis
3. Results
3.1. Fermentation Effects on GABA Content
3.2. Effect of FSC on Alcohol Metabolism
3.2.1. Effect of FSC on Blood Concentration of Alcohol and Acetaldehyde
3.2.2. Effect of FSC on Alcohol-Metabolizing Enzymes of Liver
3.3. Effect of FSC on Chronic Alcohol-Induced Hepatic Damage
3.3.1. Effect of FSC on Serum Biochemical Parameters
3.3.2. Histopathology
3.3.3. Effect of FSC on the Expression of Lipid Metabolism-Related Genes in Rat Liver
3.3.4. Effect of FSC on the Gene Expression of Anti-Inflammatory and Antioxidant Enzymes
3.4. In Vivo Acute Oral Toxicity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment (per kg b.w.) | ALP (U/L) | ALT (U/L) | AST (U/L) | ALB (U/L) |
---|---|---|---|---|
ND | 857.0 ± 7.8 b | 107.3 ± 3.8 b | 26.3 ± 5.5 c | 4.0 ± 0.1 a |
NC | 1364.7 ± 9.4 a | 169.3 ± 4.9 a | 47.3 ± 1.4 a | 4.0 ± 0.2 a |
PC | 780.8 ± 4.8 b | 102.8 ± 2.1 b | 36.5 ± 4.7 b | 3.7 ± 0.1 a |
FSC 125 | 1311.3 ± 4.1 a | 99.0 ± 9.4 b | 36.3 ± 4.4 b | 3.6 ± 0.2 a |
FSC 250 | 1018.0 ± 7.4 c | 99.0 ± 6.1 b | 37.3 ± 4.4 ab | 3.5 ± 0.1 a |
FSC 500 | 985.0 ± 9.7 b,c | 100.5 ± 9.6 b | 28.7 ± 1.2 c | 3.9 ± 0.1 a |
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Boby, N.; Lee, E.-B.; Abbas, M.A.; Park, N.-H.; Lee, S.-P.; Ali, M.S.; Lee, S.-J.; Park, S.-C. Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Smilax china Root Extract in Rats. Foods 2021, 10, 2381. https://doi.org/10.3390/foods10102381
Boby N, Lee E-B, Abbas MA, Park N-H, Lee S-P, Ali MS, Lee S-J, Park S-C. Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Smilax china Root Extract in Rats. Foods. 2021; 10(10):2381. https://doi.org/10.3390/foods10102381
Chicago/Turabian StyleBoby, Naila, Eon-Bee Lee, Muhammad Aleem Abbas, Na-Hye Park, Sam-Pin Lee, Md. Sekendar Ali, Seung-Jin Lee, and Seung-Chun Park. 2021. "Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Smilax china Root Extract in Rats" Foods 10, no. 10: 2381. https://doi.org/10.3390/foods10102381