Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of DHT-Filled Osmotic Pumps
2.2. Animal Surgery and DHT Osmotic Pump Implantation
2.3. Measurement of Serum Hormones
2.4. Histopathology
2.4.1. Decalcification of Femur
2.4.2. H&E Staining
2.5. Analysis of mRNA Expression (Semi-Quantitative PCR)
3. Statistical Analysis
4. Results
4.1. Confirmation of PCOM
4.2. Biochemical Results
4.2.1. Serum Hormonal Profiles
4.2.2. Serum Levels of TNF-α and IL-1β
4.3. Histopathological Examination
4.4. mRNA Expression of Inflammatory Cytokines and Stress-Related Peptides
5. Discussion
6. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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S.No | Gene | Forward Primer | Reverse Primer | Accession Number | Product Size |
---|---|---|---|---|---|
1 | IL-6 | TGATGGATGCTTCCAAACTG | GAGCATTGGAAGTTGGGGTA | NM_012589.2 | 230 |
2 | IL-1 β | CACCTTCTTTTCCTTCATCTTTG | GTCGTTGCTTGTCTCTCCTTGTA | NM_031512.2 | 241 |
3 | TNF-α | AAATGGGCTCCCTCTCATCAGTTC | TCTGCTTGGTGGTTTGCTACGAC | XM_008772775.2 | 111 |
4 | Nrf2 | CACATCCAGACAGACACCAGT | CTACAAATGGGAATGTCTCTGC | NM_031789 | 121 |
5 | Ucn-1 | CTCCTGGTAGCGTTGCTGCTTCTG | GCCCACCGAATCGAATATGATGC | NM_019150.1 | 339 |
6 | Gpx | GTCCACCGTGTATGCCTTCTCC | TCTCCTGATGTCCGAACTGATTGC | NM_030826.4 | 218 |
7 | β-actin | AGCCATGTACGTAGCCAT | CTCTCAGCTGTGGTGGTGAA | NM_031144.3 | 228 |
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Krishnan, A.; Muthusami, S.; Periyasamy, L.; Stanley, J.A.; Gopalakrishnan, V.; Ramachandran, I. Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology. Medicina 2020, 56, 100. https://doi.org/10.3390/medicina56030100
Krishnan A, Muthusami S, Periyasamy L, Stanley JA, Gopalakrishnan V, Ramachandran I. Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology. Medicina. 2020; 56(3):100. https://doi.org/10.3390/medicina56030100
Chicago/Turabian StyleKrishnan, Abhaya, Sridhar Muthusami, Loganayaki Periyasamy, Jone A. Stanley, Vasudevan Gopalakrishnan, and Ilangovan Ramachandran. 2020. "Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology" Medicina 56, no. 3: 100. https://doi.org/10.3390/medicina56030100