Abstract
Ten flavonoids were isolated from the ethanolic extract of propolis collected in the Yangpyeong of Korea. Their structures were elucidated by extensive spectroscopic methods and identified as pinostrobin, tectochrysin, pinobanksin 3-acetoxy-7-methyl ether, 7-methylkaempferol, pinobanksin acetate, pinobanksin 3-(2-methyl)butylate, alpinone, pinobanksin, pinocembrin, and chrysin. These compounds exhibited an inhibitory effect on DNA single strand breakage by the Fenton reaction. Several flavonoids exhibited moderate scavenging activity against 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical cations, while all flavonoids showed no activity against superoxide and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
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References
Agrawal PK (1989) Carbon-13 NMR of flavonoids. In Studies in Organic Chemistry, p.39. Elsevier Science Publishing Company Inc., New York, NY, U.S.A.
Aguero MB, Gonzalez M, Lima B, Svetaz L, Sanchez M, Zacchino S, Feresin GE, Palermo J, Wunderlin D, and Tapia A (2010) Argentinean propolis from Zuccagnia punctata Cav. (Caesalpinieae) exudates: Phytochemical characterization and antifungal activity. J Agric Food Chem 58, 194–201.
Ahn MR, Kumazawa S, Hamasaka T, Bang KS, and Nakayama T (2004) Antioxidant activity and constituents of propolis collected in various areas of Korea. J Agric Food Chem 52, 7286–7292.
Banskota AH, Tezuka Y, and Kadota S (2001) Recent progress in pharmacological research of propolis. Phytother Res 15, 561–571.
Burdock GA (1998) Review of the biological properties and toxicity of bee propolis. Food Chem Toxicol 36, 347–363.
Cheng PC and Wong G (1996) Honey bee propolis; prospects in medicine. Bee World 77, 8–15.
Choi YM, Noh DO, Cho SY, Suh HJ, Kim KM, and Kim JM (2006) Antioxidant and antimicrobial activities of propolis from several regions of Korea. LWT-Food Sci Technol 39, 756–761.
Gardana C, Scaglianti M, Pietta P, and Simonetti P (2007) Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography-tendem mass spectrometry. J Pharm Biomed Anal 45, 390–399.
Han SH, Sung KH, Yim DS, Lee SK, Cho KH, Lee CK, Ha NJ, and Kim KJ (2002) Activation of murine macrophage cell line RAW 264.7 by Korean propolis. Arch Pharm Res 25, 895–902.
Jang IW, Park JS, Kwon HC, Jung MY, and Choi DS (2009) Antimutagenic and antibacterial activities of Korean and American propolis. Korean J Food Sci Technol 41, 694–699.
Jeong CH, Bae YI, Lee HJ, and Shim KH (2003) Chemical components of propolis and its ethanolic extracts. J Korean Soc Food Sci Nutr 32, 501–505.
Jeong IY (2005) Antioxidant activity and radioprotection of two flavonoids from propolis. J Korean Soc Food Sci Nutr 34, 162–166.
Kumazawa S, Suzuki S, Ahn MR, Kamihira M, Udagawa Y, Bang KS, and Nakayama T (2006) A new chalcone from propolis collected on Jeju island, Korea. Food Sci Technol Res 12, 67–69.
Lee IK and Yun BS (2007) Highly oxygenated and unsaturated metabolites providing a diversity of hispidin class antioxidants in the medicinal mushrooms Inonotus and Phellinus. Bioorg Med Chem 15, 3309–3314.
Lee IK, Jung JY, Kim YS, Rhee MH, and Yun BS (2009) p-Terphenyls from the fruiting bodies of Paxillus curtisii and their antioxidant properties. Bioorg Med Chem 17, 4674–4680.
Perez CA, Wei Y, and Guo M (2009) Iron-binding and anti-Fenton properties of baicalein and baicalin. J Inorg Biochem 103, 326–332.
Wolniak M, Oszmianskib J, and Wawera I (2008) Solid-state NMR studies and DFT calculations of flavonoids: baicalein, baicalin, and wogonoside. Magn Reson Chem 46, 215–225.
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Han, MS., Lee, IK., Kim, YS. et al. Flavonoids from Propolis Inhibit DNA Single Strand Breakage by the Fenton Reaction. J. Korean Soc. Appl. Biol. Chem. 53, 512–515 (2010). https://doi.org/10.3839/jksabc.2010.078
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DOI: https://doi.org/10.3839/jksabc.2010.078