Authors : Pei-Ying Lo, Kang-Hsin Chen and Hui-Pi Huang
Abstract: Canine atopic dermatitis is a common pruritic skin disease which is most commonly associated with Immunoglobulin (Ig) E immune response to environmental allergens. The aims of this study were to report comparison between total serum IgE concentrations in atopic and non-atopic dogs. Comparison between results of intradermal tests and serum allergen-specific IgE arrayed protein chips was also investigated. Total serum IgE concentrations between 37 atopic dogs (92.5±102.3 μg mL-1) and 34 non-atopic dogs (16.2±30.2 μg mL-1) were significantly different (p<0.001). A positive correlation between total serum IgE concentrations and corresponding modified canine atopic dermatitis extent and severity index scores was found (r = 0.56, p<0.001) in atopic dogs. The cut-off value was 16.8 μg mL-1 with the sensitivity of 91.9% and the specificity of 85.3%. The overall sensitivity of specific IgE serum tests was 24%, specificity was 95% and efficacy was 81%. Total serum IgE concentrations could be a screening test and determine severity of atopic dermatitis in dogs with discrete clinical evaluation. However, arrayed protein chip method might not be suitable for screening functional allergen-specific IgE.
Pei-Ying Lo, Kang-Hsin Chen and Hui-Pi Huang, 2012. Comparison of Intradermal Tests, Total Serum IgE Concentrations and Allergen-Specific IgE Using an Arrayed Protein Chip in Atopic Dogs. Journal of Animal and Veterinary Advances, 11: 3298-3303.