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Semin Thromb Hemost 2010; 36(8): 845-856
DOI: 10.1055/s-0030-1267038
© Thieme Medical Publishers

The Plasma Microparticle Proteome

Kristina M. Little1 , 2 , David M. Smalley3 , Nancy L. Harthun4 , Klaus Ley1
  • 1Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California
  • 2Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia
  • 3Ernest G. Stillman Proteomics Laboratory, Maine Institute for Human Genetics and Health, Bangor, Maine
  • 4Johns Hopkins School of Medicine, Baltimore, Maryland
Further Information

Publication History

Publication Date:
03 November 2010 (online)

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ABSTRACT

All cell types shed ectosomes and exosomes, collectively known as microparticles (MP; 0.1 to 1.5 μm in diameter), when activated or stressed; normal human plasma contains ~2 μg MP protein/mL. The cellular composition of plasma MP is altered in many diseases, including acute coronary syndrome, diabetes mellitus, sepsis, and sickle cell disease. We measured the plasma MP protein composition of 42 patients (median age 69.5 years, most with cardiovascular disease) by label-free liquid chromatography coupled to tandem mass spectrometry. Among 458 proteins detected with high confidence (identified by at least two unique peptides with SEQUEST XCor (Thermo Electron Corp., San Jose, CA) ≥2.0, 2.2, and 3.3 for charge states +1, +2, and +3, respectively), 130 were present in most patients, representing a “core” set of plasma MP proteins. This core is enriched in cytoskeletal, integrin complex, and hemostasis proteins, and spectral counts of several proteins correlate with patient age and gender. We conclude that the MP proteome may be a useful and reliable source of biologically relevant disease biomarkers.

KEYWORDS

Mass spectrometry - microparticles - proteomics

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Klaus LeyM.D. 

Head, Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology

9420 Athena Circle, La Jolla, CA 92037

Email: klaus@liai.org

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