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Horm Metab Res 2013; 45(08): 599-604
DOI: 10.1055/s-0033-1341511
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

MicroRNA Expressions in PMBCs, CD4+, and CD8+ T-Cells from Patients Suffering from Autoimmune Addison’s Disease

C. Bernecker
1   Department of Endocrinology and Diabetes, Medical Faculty, Heinrich-Heine University Düsseldorf, Germany
,
F. Halim
1   Department of Endocrinology and Diabetes, Medical Faculty, Heinrich-Heine University Düsseldorf, Germany
,
M. Haase
1   Department of Endocrinology and Diabetes, Medical Faculty, Heinrich-Heine University Düsseldorf, Germany
,
H. S. Willenberg
1   Department of Endocrinology and Diabetes, Medical Faculty, Heinrich-Heine University Düsseldorf, Germany
,
M. Ehlers
1   Department of Endocrinology and Diabetes, Medical Faculty, Heinrich-Heine University Düsseldorf, Germany
,
M. Schott
1   Department of Endocrinology and Diabetes, Medical Faculty, Heinrich-Heine University Düsseldorf, Germany
› Author Affiliations
Further Information

Publication History

received 25 January 2013

accepted 14 March 2013

Publication Date:
15 April 2013 (online)

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Abstract

Autoimmune Addison’s disease (AD) is a rare but potentially life threatening disease. The exact etiology of the immune response to the adrenal gland is still unknown. MicroRNAs (miRNAs) critically control gene-expression and play an important role in regulating the immune response. The aim of this study was to determine key immunoregulatory miRNAs influencing autoimmune adrenal insufficiency. For this purpose selected miRNAs were amplified by a semiquantitative SYBR Green PCR from blood mononuclear cells and after purification from CD4+ and CD 8+ cells of 6 patients with autoimmune adrenal insufficiency and 10 healthy controls. In CD4+ T-cells miRNA 181a*_1 (18.02 in AD vs. 11.99 in CG, p=0.0047) is significantly increased whereas miRNA 200a_1 (12.48 in AD vs. 19.40 in CG, p=0.0003) and miRNA 200a_2* (8.59 in AD vs. 17.94 in CG, p=0.0160) are significantly decreased. miRNA 200a_1 (12.37 in AD group vs. 18.12 in control group, p=0.001) and miRNA 200a_2* (10.72 in AD group vs. 17.84 in control group, p=0.022) are also significantly decreased in CD8+ T-cells. This study could show for the first time a significant change of three defined miRNAs in PBMCs, CD4+, and CD8+ T-cells of autoimmune AD patients in vivo. These data may help to better understand the cause of the autoimmune processes leading to autoimmune AD. They extend our very limited knowledge concerning miRNAs in autoimmune Addison’s disease.

Key words

micro-RNA - immunology - autoimmune primary adrenal insufficieny - autoimmune Addison’s disease
 

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