Molekulare Radiologie.
Teil II: Molekulare Bildgebung

 

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Zusammenfassung.

Es werden die wichtigsten Strategien zur Molekularen Bildgebung vorgestellt. Mittels moderner bildgebender Verfahren, d. h. mit SPECT, PET, MRT und hochentwickelten optischen Methoden, können die Vorgänge auf molekularer Ebene in vitro und in vivo abgebildet werden. Die Möglichkeit die Expression von Genen, therapeutischer DNA und von klassischen Pharmaka in vivo abzubilden, eröffnet dabei völlig neue Ansätze für die Erforschung, Diagnostik und Therapie von Erkrankungen. Der aktuelle Stand dieser Entwicklung wird beschrieben sowie potenzielle Anwendungsbereiche und Entwicklungsmöglichkeiten umrissen.

Molecular Radiology. Part ll: Molecular Imaging.

The most important strategies for molecular imaging are presented. Processes can now be imaged in vitro and in vivo at the molecular level with the help of modern procedures such as SPECT, PET, MRI, and highly developed optical methods. The ability to view the expression of genes, the pharmacokinetics of gene therapy vectors, of therapeutic DNA, and of classical pharmacons in vivo opens up completely new perspectives for the research on, diagnosis of, and therapy for diseases. The current status of these developments are described, potential fields of use and possibilities for further development are outlined

Literatur

• 1 Alfke H, Kalinowski M, Nocken F, Klose K J. Eine Übersicht über die Molekulare Radiologie. Teil 1: Gentherapie.  Fortschr Röntgenstr. 2000;  172 949-956
  PubMedGoogle Scholar
• 2 Calvo B F, Semelka R C. Beyond anatomy. MR imaging as a molecular diagnostic tool.  Surg Oncol Clin North Am. 1999;  8 171-183
  PubMedGoogle Scholar
• 3 Weissleder R. Molecular Imaging: Exploring the next frontier.  Radiology. 1999;  212 609-614
  CrossrefPubMedGoogle Scholar
• 4 Gambhir S S, Barrio J R, Herschman H R, Phelps M E. Assays for noninvasive imaging of reporter gene expression.  Nucl Med Biol. 1999;  26 481-490
  CrossrefPubMedGoogle Scholar
• 5 Bogdanov A, Weissleder R. The development of in vivo imaging systems to study gene expression.  Trends Biotechnol. 1998;  16 5-10
  PubMedGoogle Scholar
• 6 Zimmer C, Weissleder R, O’Connor D, LaPointe L, Brady T J, Enochs W S. Cerebral iron oxide distribution: In vivo mapping with MR imaging.  Radiology. 1995;  196 521-527
  PubMedGoogle Scholar
• 7 Weissleder R, Moore A, Mahmood U, Bhorade R, Benveniste H, Chiocca E A. et al . In vivo magnetic resonance imaging of transgene expression.  Nat Med. 2000;  6 351-354
  CrossrefPubMedGoogle Scholar
• 8 Weissleder R, Simonova M, Bogdanova A, Bredow S, Enochs W S, Bogdanov A. MR Imaging and scintigraphy of gene expression through melanin induction.  Radiology. 1998;  204 425-429
  PubMedGoogle Scholar
• 9 Ennochs W S, Petherick P, Bogdanova A, Mohr U, Weissleder R. Paramagnetic metal scavenging by melanin: MR imaging.  Radiology. 1997;  204 417-423
  PubMedGoogle Scholar
• 10 Saito Y, Price R W, Rottenberg D A, Fox J J, Su T -L, Watanabe K A, Philips F A. Quantitative autoradiographic mapping of herpes simplex virus encephalitis with radiolabeled antiviral drugs.  Science. 1982;  217 1151-1153
  PubMedGoogle Scholar
• 11 Tjuvajev J G, Stockhammer G, Desai R, Uehara H, Watanabe K, Gansbacher G. et al . Imaging the expression of transfected genes in vivo.  Cancer Res. 1995;  55 6126-6132
  PubMedGoogle Scholar
• 12 Louie A Y, Hüber M M, Ahrens E T, Rothbächer U, Moats R, Jacobs R E. et al . In vivo visualization of gene expression using magnetic resonance imaging.  Nat Biotech. 2000;  18 321-325
  CrossrefPubMedGoogle Scholar
• 13 Klose K J. Biosignale für die radiologische Forschung.  Fortschr Röntgenstr. 2000;  172 573-575
  PubMedGoogle Scholar
• 14 Lanza G M, Abendschein D R, Hall C S, Scott M J, Scherrer D E, Houseman A, Miller J G, Wickline S A. In vivo molecular imaging of stretch-induced tissue factor in carotid arteries with ligand-targeted nanoparticles.  J Am Soc Echocardiog. 2000;  13 608-814
  PubMedGoogle Scholar
• 15 Yu Y, Annala A J, Barrio J R, Toyokuni T, Satyamurthy N, Namavari M. et al . Quantification of target gene expression by imaging reporter gene expression in living animals.  Nat Med. 2000;  6 933-937
  PubMedGoogle Scholar
• 16 Contag P R, Olomu N, Stevenson D K, Contag C H. Bioluminescent indicators in living mammals.  Nat Med. 1998;  4 245-247
  PubMedGoogle Scholar
• 17 Yang M, Baranov E, Jiang P, Sun F -X, Li X -M, Li L. et al . Whole-body optical imaging of green fluorescent protein-expressing tumors and metastasis.  Proc Natl Acad Sci USA. 2000;  97 1206-1211
  CrossrefPubMedGoogle Scholar
• 18 Weissleder R, Tung C H, Mahmood U, Bogdanov A. In vivo imaging of tumors with protease-activated near-infrared fluorescent probes.  Nat Biotechnol. 1999;  17 375-378
  CrossrefPubMedGoogle Scholar
• 19 Tavitian B, Terrazzino S, Kühnast B, Marzabal S, Stettler O, Dollé F. et al . In vivo imaging of oligonucleotides with positron emission tomography.  Nat Med. 1998;  4 467-471
  PubMedGoogle Scholar
• 20 de Marco G, Bogdanov A, Marecos E, Moore A, Simonova M, Weissleder R. MR imaging of gene delivery to the central nervous system with an artificial vector.  Radiology. 1998;  208 65-71
  PubMedGoogle Scholar
• 21 Seidenwurm D J. Imaging-guided gene therapy in the central nervous system.  Radiology. 1998;  208 17-18
  PubMedGoogle Scholar
• 22 Martínez-Salas E. Internal ribosome entry site biology and its use in expression vectors.  Curr Opin Biotech. 1999;  10 458-464
  PubMedGoogle Scholar
• 23 Buckley R H. Gene therapy for human SCID: Dreams become reality.  Nat Med. 2000;  6 623-624
  PubMedGoogle Scholar
• 24 Dick J E. Gene therapy turns the corner.  Nat Med. 2000;  6 624-626
  PubMedGoogle Scholar
• 25 Yeh T C, Zhang W, Ildstadt S T, Ho C. Intracellular labeling of t-cells with superparamagnetic contrast agents.  MRM. 30;  1993 617-625
  PubMedGoogle Scholar
• 26 Lewin M, Carlesso N, Tung C -H, Tang X -W, Cory D, Scadden D T. et al . Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells.  Nat Biotech. 2000;  18 410-414
  CrossrefPubMedGoogle Scholar
• 27 Newman S P, Wilding I R. Imaging techniques for assessing drug delivery in man.  PSTT. 1999;  2 181-189
  PubMedGoogle Scholar
• 28 Melia C D, Rajabi-Siahboomi A R, Bowtell R W. Magnetic resonance imaging of controlled release pharmaceutical dosage forms.  PSTT. 1998;  1 32-39
  PubMedGoogle Scholar
• 29 Langer R. Drug delivery and targeting.  Nature. 1998;  392 5-10
  PubMedGoogle Scholar
• 30 Sullivan D C. Biomedical symposium: Visualizing the future of biology and medicine.  Radiology. 2000;  215 634-638
  PubMedGoogle Scholar

Dr. med. Heiko Alfke

Abteilung Strahlendiagnostik
Klinikum der Philipps Universität Marburg

Baldingerstraße

35043 Marburg

Phone: 06421/286-6231

Fax: 06421/286-8959

Email: alfke@mailer.uni-marburg.de