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Planta Med 2007; 73(13): 1402-1406
DOI: 10.1055/s-2007-990237
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Bioactive Capsular Polysaccharide from the Thermophilic Cyanophyte/Cyanobacterium Mastigocladus laminosus - Cytotoxic Properties

Vincent Gloaguen1 , Henri Morvan2 , Lucien Hoffmann3 , Odile Sainte Catherine4 , Michel Kraemer4 , Pierre Krausz1
  • 1Laboratoire de Chimie des Substances Naturelles, Université de Limoges, Limoges, France
  • 2Groupe de Recherche en Glycobiologie et Physiologie Végétale, Université d'Artois, Lens, France
  • 3Département ‘Environnement et agro-biotechnologies’ (EVA), Centre de Recherche Public-Gabriel Lippmann, Belvaux, Grand-duché de Luxembourg
  • 4Laboratoire d'Oncologie Cellulaire et Moléculaire, Université Paris 13, Paris, France
Further Information

Publication History

Received: May 22, 2007 Revised: August 22, 2007

Accepted: September 8, 2007

Publication Date:
09 October 2007 (online)

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Abstract

The capsular polysaccharide produced by the thermophilic blue green alga/cyanobacterium Mastigocladus laminosus was tested for its cytotoxic activity against the A431 human epidermoid carcinoma cell line. This polysaccharide inhibited the proliferation of A431 cells in a dose-dependent manner with an IC50 value of 50 μg mL-1. In addition, this polysaccharide strongly inhibited A431 cell migration and invasion. Preliminary experiments showing that secretion of metalloproteinases MMP2 and MMP9 by A431 tumour cells was inhibited by this polysaccharide suggest that this mechanism of action could play a role in its anti-migration and anti-invasive properties. Acid hydrolysis of the polysaccharide produced specific oligosaccharides which conserved - at similar concentrations - their cytotoxic, anti-migration and anti-invasion properties; in this case, the mechanism of action was nevertheless uncorrelated to the decrease of metalloproteinase expression.

Key words

Mastigocladus laminosus - blue-green alga - cyanobacterium - capsular polysaccharide - carcinoma - cytotoxic properties

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Dr. Vincent Gloaguen

Laboratoire de Chimie des Substances Naturelles

Faculté des Sciences et Techniques

Université de Limoges

123 rue A. Thomas

87060 Limoges

France

Phone: +33-5-5545-7481

Email: vincent.gloaguen@unilim.fr

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