Abstract
Large animal models are an important preclinical tool for the evaluation of new interventions and their translation into clinical practice. The pig is a widely used animal model in multiple clinical fields, such as cardiology and orthopedics, and has been at the forefront of testing new therapeutics, including cell-based therapies. In the clinic, mesenchymal stem cells (MSCs) are used autologously, therefore isolated, and administrated into the same patient. For successful clinical translation of autologous approaches, the porcine model needs to test MSC in a similar manner. Since a limited number of MSCs can be isolated directly from the bone marrow, culturing techniques are needed to expand the population in vitro prior to therapeutic application. Here, we describe a protocol specifically tailored for the isolation and propagation of porcine-derived bone marrow MSCs.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pittenger MF, Martin BJ (2004) Mesenchymal stem cells and their potential as cardiac therapeutics. Circ Res 95:9–20
Gnecchi M, He H, Noiseux N et al (2006) Evidence supporting paracrine hypothesis for Akt-modified mesenchymal stem cell-mediated cardiac protection and functional improvement. FASEB J 20:661–669
Gnecchi M, Zhang Z, Ni A et al (2008) Paracrine mechanisms in adult stem cell signaling and therapy. Circ Res 103:1204–1219
van den Akker F, de Jager SC, Sluijter JP (2013) Mesenchymal stem cell therapy for cardiac inflammation: immunomodulatory properties and the influence of toll-like receptors. Mediators Inflamm 2013:181020
Noort WA, Feye D, Van Den Akker F et al (2010) Mesenchymal stromal cells to treat cardiovascular disease: strategies to improve survival and therapeutic results. Panminerva Med 52:27–40
Magne D, Vinatier C, Julien M et al (2005) Mesenchymal stem cell therapy to rebuild cartilage. Trends Mol Med 11:519–526
Knight MN, Hankenson KD (2013) Mesenchymal stem cells in bone regeneration. Adv Wound Care (New Rochelle) 2:306–316
Le Blanc K, Frassoni F, Ball L et al (2008) Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study. Lancet 371:1579–1586
Ball LM, Bernardo ME, Roelofs H et al (2007) Cotransplantation of ex vivo expanded mesenchymal stem cells accelerates lymphocyte recovery and may reduce the risk of graft failure in haploidentical hematopoietic stem-cell transplantation. Blood 110:2764–2767
Dixon JA, Spinale FG (2009) Large animal models of heart failure: a critical link in the translation of basic science to clinical practice. Circ Heart Fail 2:262–271
van der Spoel TI, Jansen of Lorkeers SJ, Agostoni P et al (2011) Human relevance of pre-clinical studies in stem cell therapy: systematic review and meta-analysis of large animal models of ischaemic heart disease. Cardiovasc Res 91:649–658
van der Spoel TI, Vrijsen KR, Koudstaal S et al (2012) Transendocardial cell injection is not superior to intracoronary infusion in a porcine model of ischaemic cardiomyopathy: a study on delivery efficiency. J Cell Mol Med 16:2768–2776
Sahni D, Kaur GD, Jit H et al (2008) Anatomy & distribution of coronary arteries in pig in comparison with man. Indian J Med Res 127:564–570
Li WJ, Chiang H, Kuo TF et al (2009) Evaluation of articular cartilage repair using biodegradable nanofibrous scaffolds in a swine model: a pilot study. J Tissue Eng Regen Med 3:1–10
Noort WA, Oerlemans MI, Rozemuller H et al (2012) Human versus porcine mesenchymal stromal cells: phenotype, differentiation potential, immunomodulation and cardiac improvement after transplantation. J Cell Mol Med 16:1827–1839
Acknowledgments
This work is part of the Project P1.04 SMARTCARE of the BioMedical Materials institute, co-funded by the Dutch Ministry of Economic Affairs, Agriculture and Innovation. The financial contribution of the Dutch Heart Foundation is gratefully acknowledged. This work was further supported by a grant from the Alexandre Suerman program for MD/PhD students of the University Medical Center Utrecht, the Netherlands, the ZonMw-TAS program (#116002016) and the Netherlands CardioVascular Research Initiative (CVON): the Dutch Heart Foundation, Dutch Federation of University Medical Centers, the Netherlands Organization for Health Research and Development, and the Royal Netherlands Academy of Sciences.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Feyen, D.A.M., van den Akker, F., Noort, W., Chamuleau, S.A.J., Doevendans, P.A., Sluijter, J.P.G. (2016). Isolation of Pig Bone Marrow-Derived Mesenchymal Stem Cells. In: Gnecchi, M. (eds) Mesenchymal Stem Cells. Methods in Molecular Biology, vol 1416. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3584-0_12
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3584-0_12
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3582-6
Online ISBN: 978-1-4939-3584-0
eBook Packages: Springer Protocols