Abstract
Experimentation with cell cultures is widespread in different areas of basic science as well as in the development of biotechnology applied to medicine. Cellular models are applied to the study of respiratory diseases. In this chapter, we present a protocol of basic cell culture using A549 Human lung adenocarcinoma epithelial cells as model. Corticosteroid therapy is used to treat respiratory diseases such asthma. Thus, we also describe a protocol of lung epithelial cell culture treated with dexamethasone to illustrate an example of monitoring the effects of a drug in a lung cell culture assay.
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Acknowledgments
This work was supported by grants of the Junta de Castilla y León ref. GRS1047/A/14 and BIO/SA73/15; and by the project “Efecto del Ácido Retinóico en la enfermedad alérgica. Estudio transcripcional y su traslación a la clínica,” PI13/00564, integrated into the “Plan Estatal de I + D + I 2013–2016” and cofunded by the “ISCIII-Subdirección General de Evaluación y Fomento de la investigación” and the European Regional Development Fund (FEDER).
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Marcos-Vadillo, E., García-Sánchez, A. (2016). Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell. In: Isidoro García, M. (eds) Molecular Genetics of Asthma. Methods in Molecular Biology, vol 1434. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3652-6_12
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DOI: https://doi.org/10.1007/978-1-4939-3652-6_12
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3650-2
Online ISBN: 978-1-4939-3652-6
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