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Tissue-Specific KO of ECM Proteins

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Extracellular Matrix Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 522))

Summary

Nearly 20 years after its first description, gene targeting and generation of transgenic mice by homologous recombination in embryonic stem cells still are cutting edge tools for the postgenomic era. Understanding the function of the large number of genes encoding extracellular matrix proteins and their cellular receptors appears a daunting task that can very much profit from a genetic approach. The generation of new mutant alleles remains essential to define the different biochemical properties of such proteins. While in the past, gene targeting represented a complex procedure, restricted to few laboratories, recent breakthroughs, such as the publication of the mouse genome sequence and the perfection of recombineering techniques in bacteria, made generation of transgenic mice faster and easier. This chapter will thus focus on the recent advances in gene-targeting technology with a special eye on the study of genes involved in cell adhesion and migration.

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Author information

Authors and Affiliations

  1. Department of Genetics, Biology and Biochemistry, Molecular Biotechnology Center, University of Torino, Via Nizza 52., 10126, Torino, Italy

    Emilio Hirsch

Authors
  1. Mara Brancaccio
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  2. Emila Turco
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  3. Emilio Hirsch
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Corresponding author

Correspondence to Emilio Hirsch .

Editor information

Editors and Affiliations

  1. Gene Therapy Inst., Hadassah Univ. Hospital, Jerusalem, 91 120, Israel

    Sharona Even-Ram

  2. Lombardi Comprehensive Cancer Ctr., Georgetown University, Reservoir Rd. NW., 3970, Washington, 20057-1468, U.S.A.

    Vira Artym

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Brancaccio, M., Turco, E., Hirsch, E. (2009). Tissue-Specific KO of ECM Proteins. In: Even-Ram, S., Artym, V. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology, vol 522. Humana Press. https://doi.org/10.1007/978-1-59745-413-1_2

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  • DOI: https://doi.org/10.1007/978-1-59745-413-1_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-984-0

  • Online ISBN: 978-1-59745-413-1

  • eBook Packages: Springer Protocols

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