Abstract
Mitogen-activated protein kinase (MAPK) signaling influences a variety of cellular responses, ranging from stimulation of cell proliferation to induction of senescence and/or apoptosis. Ca2+ is a ubiquitous intracellular signaling molecule that controls multiple processes in cells. Published evidence has identified both direct and indirect interactions between the Ca2+ and MAPK signaling pathways. Here, we describe assays to accurately determine the effect of changes in intracellular Ca2+ concentration on MAPK activation.
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References
Cuevas, B. D., Abell, A. N. and Johnson, G. L. (2007). Role of mitogen-activated protein kinase kinase kinases in signal integration. Oncogene 26, 3159–71.
McKay, M. M. and Morrison, D. K. (2007). Integrating signals from RTKs to ERK/MAPK. Oncogene 26, 3113–21.
Casar, B., Pinto, A. and Crespo, P. (2008). Essential role of ERK dimers in the activation of cytoplasmic but not nuclear substrates by ERK-scaffold complexes. Mol Cell 31, 708–21.
Agell, N., Bachs, O., Rocamora, N. and Villalonga, P. (2002). Modulation of the Ras/Raf/MEK/ERK pathway by Ca2+ and calmodulin. Cell Signal 14, 649–54.
Rosen, L. B., Ginty, D. D., Weber, M. J. and Greenberg, M. E. (1994). Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras. Neuron 12, 1207–21.
Medema, J. P., Sark, M. W., Backendorf, C. and Bos, J. L. (1994). Calcium inhibits epidermal growth factor-induced activation of p21ras in human primary keratinocytes. Mol Cell Biol 14, 7078–85.
Berridge, M. J., Lipp, P. and Bootman, M. D. (2000). The versatility and universality of calcium signaling. Nat Rev Mol Cell Biol 1, 11–21.
Bardo, S., Cavazzini, M. G. and Emptage, N. (2006). The role of the endoplasmic reticulum Ca2+ store in the plasticity of central neurons. Trends Pharmacol Sci 27, 78–84.
Endo, M. (2006). Calcium ion as a second messenger with special reference to excitation-contraction coupling. J Pharmacol Sci 100, 519–24.
Pressman, B. C. (1976). Biological applications of ionophores. Annu Rev Biochem 45, 501–30.
Acknowledgments
We thank Zhigang Li for critically reviewing the text prior to submission and other members of the Sacks laboratory, past and present, for insightful discussions. Work in the authors’ laboratory is funded by the National Institutes of Health (to D.B.S) and the Department of Defense Breast Cancer Research Program (to C.D.W).
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White, C.D., Sacks, D.B. (2010). Regulation of MAP Kinase Signaling by Calcium. In: Seger, R. (eds) MAP Kinase Signaling Protocols. Methods in Molecular Biology, vol 661. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-795-2_9
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DOI: https://doi.org/10.1007/978-1-60761-795-2_9
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-60761-795-2
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