Abstract
The suction blister model was originally developed for the separation of viable epidermis from dermis by Kiistala (1). Since its development, its use has been expanded and applied to several other applications. One new application assesses the collagen synthesis rate in the human skin in vivo by using the suction blister fluid collected in an assay of collagen propeptides (2). This method is sensitive and can detect changes in collagen synthesis owing to various diseases and topical or systemic therapies. Other applications of the suction blister technique include measurements of pharmacological agents or their derivatives from interstitial fluid, and assays of various enzymes, cytokines, and so on (3-7). Additionally, the suction blister technique has even been used to treat vitiligo by collecting living melanocytes from blister roofs from healthy skin and injecting these into blister cavities induced in diseased skin.
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References
Kiistala, U. (1968) Suction blister device for separating viable epidermis from dermis. J. Invest. Dermatol. 50, 129ā137.
Oikarinen, A., Autio, P., Kiistala, U., Risteli, L., and Risteli, J. (1992) A new method to measure type I and III collagen synthesis in human skin in vivo: demonstration of decreased collagen synthesis after topical glucocorticoid treatment. J. Invest. Dermatol. 98, 220ā225.
Ingemansson-Nordqvist, B., Kiistala, U., and Rorsman, H. (1967) Culture of adult human epidermal cells obtained from roofs of suction blisters. Acta Dermatol. Venereol. 47, 237ā240.
Averbeck, D., Averbeck, S., Blais, J., Moysan, A., HĆ¼ppe, G., MorliĆ©re, P., Prognon, P., Vigny, P., and Dubertret, L. (1989) Suction blister fluid: its use for pharmacodynamic and toxicological studies of drugs and metabolites in vivo in human skin after topical or systemic administration, in Models in Dermatology, vol. 4 (Maibach, H. I. and Lowe, N. J., eds.), Karger, Basel, pp. 5ā11.
Benfeldt, E., Serup, J., and MennĆ©, T. (1999) Microdialysis vs. suction blister technique for in vivo sampling of pharmacokinetics in the human dermis. Acta Dermatol. Venereol. 79, 338ā342.
Oikarinen, A., KylmƤniemi, M., Autio-Harmainen, H., Autio, P., and Salo, T. (1993) Demonstration of 72-kDa and 92-kDa forms of type IV collagenase in human skin: variable expression in various blistering diseases, induction during re-epithelialization and decrease by topical glucocorticoids. J. Invest. Dermatol. 101, 205ā210.
KylmƤniemi, M., Autio, P., and Oikarinen, A. (1994) Interleukin 1alfa (IL-1alfa) in human skin in vivo. Lack of correlation to markers of collagen metabolism. Acta Dermatol. Venereol. 74, 364ā367.
Kiistala, U. and Mustakallio, K. K. (1967) Dermo-epidermal separation with suction: electron microscopic and histochemical study of initial events of blistering on human skin. J. Invest. Dermatol. 48, 466ā477.
Oikarinen, A., Savolainen, E.-R., Tryggvason, K., Foidart, J.-M., and Kiistala, U. (1982) Basement membrane components and galactosylhydroxylysyl glucosyltransferase in suction blisters of human skin. Br. J. Dermatol. 106, 257ā266.
Kainulainen, T., HƤkkinen, L., Hamidi, S., Larjava, K., Kallioinen, M., Peltonen J., Salo, T., Larjava, H., and Oikarinen, A. (1998) Laminin-5 expression is independent of the injury and microenvironment during re-epithelialization of wounds. J. Histochem. Cytochem. 46, 353ā360.
Leivo, T., Kiistala, U., Vesterinen, M., Owaribe, K., Burgeson, R. E., Virtanen, I., and Oikarinen, A. (2000) Re-epithelialization rate and protein expression in suction-induced wound model: comparison between intact blisters, open wounds and calcipotrial-pretreated open wounds. Br. J. Dermatol. 142, 991ā1002.
Kiistala, U. (1972) Dermal-epidermal separation II. External factors in suction blister formation with special reference to the effects of temperature. Ann. Clin. Res. 4, 236ā246.
Koivukangas, V., Annala, A.-P., Salmela, P. I., and Oikarinen, A. (1999) Delayed restoration of epidermal barrier function after suction blister injury in patients with diabetes mellitus. Diabet. Med. 16, 563ā567.
Koivukangas, V. and Oikarinen, A. (1998) Effects of PUVA and UVB treatment on restoration of epidermal barrier function and vascular response after suction blister injury in human skin in vivo. Photodermatol. Photoimmunol. Photomed. 14, 119ā124.
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Ā© 2003 Humana Press Inc., Totowa, NJ
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Koivukangas, V., Oikarinen, A. (2003). Suction Blister Model of Wound Healing. In: DiPietro, L.A., Burns, A.L. (eds) Wound Healing. Methods in Molecular Medicineā¢, vol 78. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-332-1:255
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DOI: https://doi.org/10.1385/1-59259-332-1:255
Publisher Name: Humana Press, Totowa, NJ
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