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Forward Genetic Screening of Insertional Mutants

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 323))

Abstract

Insertional mutagenesis has contributed to the success of forward genetics in Arabidopsis thaliana. The availability of large collections of lines mutagenized by either transposon or T-DNA insertions, in combination with the systematic sequencing of insertion/genome junctions, enables the identification of mutations for any given gene in the nuclear genome of this plant species. Protocols for the identification and confirmation of mutations by forward genetics, the isolation of insertion/genome junctions, and the stabilization of transposon-induced mutations are provided, together with an overview of available insertional mutant collections and of their characteristics.

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Author information

Authors and Affiliations

  1. Botanisches Institut der Universität zu Köln, Köln, Germany

    Anja Schneider

  2. Abteilung für Pflanzenzüchtung und Ertragsphysiologie, Max-Planck-Institut für Züchtungsforschung, Köln, Germany

    Dario Leister

Authors
  1. Anja Schneider
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  2. Dario Leister
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Editor information

Editors and Affiliations

  1. Dept. of Biotechnology, Instituto Nacional de Investigacion y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain

    Julio Salinas PhD

  2. Dept. de Genetica Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Campus de Cantoblanco, Madrid, Spain

    Jose J. Sanchez-Serrano PhD

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© 2006 Humana Press Inc.

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Schneider, A., Leister, D. (2006). Forward Genetic Screening of Insertional Mutants. In: Salinas, J., Sanchez-Serrano, J.J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 323. Humana Press. https://doi.org/10.1385/1-59745-003-0:147

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  • DOI: https://doi.org/10.1385/1-59745-003-0:147

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-395-4

  • Online ISBN: 978-1-59745-003-4

  • eBook Packages: Springer Protocols

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