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26.05.2022 | Original Contribution

Amino acid-balanced diets improved DSS-induced colitis by alleviating inflammation and regulating gut microbiota

verfasst von: Sen Li, Shuya Yang, Yu Zhang, Kai Huang, Ting Liang, Yu Chen, Yingjie Guan, Ruizhi Shang, Tong Guan, Jiang Wu, Yingwei Chen, Xiao Guan

Erschienen in: European Journal of Nutrition | Ausgabe 7/2022

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Abstract

Purpose

Inflammatory bowel disease (IBD) is a multifactorial chronic disease of the gastrointestinal tract. Dietary intervention in the treatment of IBD has gradually attracted more attention. In this study, amino acid-balanced diets (AABD) based on grains were developed and their influences on the regulation of IBD were investigated.

Methods

Dextran sodium sulfate (DSS)-induced acute colitis mice model was employed to evaluate the effects of AABD. Pathological symptoms, intestinal inflammation, gut barrier proteins and gut microbiota were determined after AABD intake.

Results

It was shown that AABD alleviated the symptoms of colitis by reducing the histological scores of mice colon, suppressing the expression of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and upregulating the expression of tight junction proteins. Analysis of gut microbiota revealed that AABD altered the structure of gut microbiota by decreasing the abundance and richness of harmful bacteria induced by DSS (Escherichia-Shigella, Parasutterella, etc.) and increasing that of beneficial bacteria (Akkermansia, etc.). Correlation analysis indicated that the alterations of pro-inflammatory factors were related with the change of microbiota, suggesting that the inhibitory effects of AABD on inflammation might be due to its regulation gut microbiota.

Conclusion

The AABD could efficiently mitigate colitis, and this study indicated that AABD could be applied as a promising dietary regulation strategy of IBD.

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Titel: Amino acid-balanced diets improved DSS-induced colitis by alleviating inflammation and regulating gut microbiota
verfasst von: Sen Li
Shuya Yang
Yu Zhang
Kai Huang
Ting Liang
Yu Chen
Yingjie Guan
Ruizhi Shang
Tong Guan
Jiang Wu
Yingwei Chen
Xiao Guan
Publikationsdatum: 26.05.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 7/2022
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-022-02906-y

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Amino acid-balanced diets improved DSS-induced colitis by alleviating inflammation and regulating gut microbiota