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International Ophthalmology

Erschienen in:

01.12.2024 | Original Paper

CircRNA HLCS regulates lens epithelial cell apoptosis via miR-338-3p/BPNT1 axis

verfasst von: Lianyi Sun, Fengzhi Li, Shuwei Bai, Chunchao Bi

Erschienen in: International Ophthalmology | Ausgabe 1/2024

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Abstract

Purpose

The purpose of this study was to investigate the effect of circ_HLCS on age-related cataract (ARC).

Methods

Circ_HLCS, microRNA (miR)-338-3p, and bisphosphate 3′-nucleotidase 1 (BPNT1) were quantified by quantitative real-time polymerase chain reaction or western blot. Cell proliferation and cell viability were assessed by the 5-Ethynyl-2′-deoxyuridinr and cell counting kit-8 assays. Cell apoptosis was detected by flow cytometry. Targeted correlations among circ_HLCS, miR-338-3p, and BPNT1 were verified by the dual-luciferase reporter and RNA pull-down assays.

Results

circ_HLCS was diminished in ARC tissues and UV-treated SRA01/04 cells. Elevated content of circ_HLCS undermined UV-induced cell proliferation inhibition and apoptosis. Mechanistically, circ_HLCS directly targeted miR-338-3p, and circ_HLCS regulated BPNT1 expression through miR-338-3p. Furthermore, reduction of miR-338-3p ameliorated UV-induced SRA01/04 cell damage by increasing BPNT1 expression.

Conclusion

Taken together, these data suggested that circ_HLCS inhibited apoptosis of UV-treated SRA01/04 cells by miR-338-3p/BPNT1 axis. Therefore, circ_HLCS might be a potential therapeutic target for ARC.

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Titel: CircRNA HLCS regulates lens epithelial cell apoptosis via miR-338-3p/BPNT1 axis
verfasst von: Lianyi Sun
Fengzhi Li
Shuwei Bai
Chunchao Bi
Publikationsdatum: 01.12.2024
Verlag: Springer Netherlands
Erschienen in: International Ophthalmology / Ausgabe 1/2024
Print ISSN: 0165-5701
Elektronische ISSN: 1573-2630
DOI: https://doi.org/10.1007/s10792-024-03082-0

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