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European Journal of Nutrition

Erschienen in:

26.07.2023 | Original Contribution

Consumption of honey ameliorates lipopolysaccharide-induced intestinal barrier dysfunction via upregulation of tight junction proteins

verfasst von: Sandip Nathani, Neeladrisingha Das, Parul Katiyar, Bhairavnath Waghmode, Debabrata Sircar, Partha Roy

Erschienen in: European Journal of Nutrition | Ausgabe 7/2023

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Abstract

Purpose

The leaky gut barrier is an important factor leading to various inflammatory gastrointestinal disorders. The nutritional value of honey and variety of its health benefits have long been recognized. This study was undertaken to assess the role of Indian mustard honey in preventing lipopolysaccharide (LPS)-induced intestinal barrier dysfunction using a combination of in vitro and in vivo experimental model systems.

Methods

LPS was used to induce intestinal barrier damage in a trans-well model of Caco-2 cells (1 µg/ml) and in Swiss albino mice (5 mg/kg body weight). Gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) were used to analyse sugar and phenolic components in honey samples. The Caco-2 cell monolayer integrity was evaluated by transepithelial electrical resistance (TEER) and paracellular permeability assays. The histopathology of intestinal tissue was analysed by haematoxylin and eosin dual staining. The quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to quantify the transcription of genes. The protein expression was analysed by immunofluorescence, western blot and ELISA-based techniques.

Results

The in vitro data showed that honey prevented LPS-induced intestinal barrier dysfunction dose dependently as was measured by TEER and paracellular flux of FITC-dextran dye. Further, the in vivo data showed a prophylactic effect of orally administered honey as it prevented the loss of intestinal barrier integrity and villus structure. The cellular localization and expression of tight junction (TJ) proteins were upregulated along with downregulation of pro-inflammatory cytokines in response to the administration of honey with LPS.

Conclusions

The findings of this study suggest a propitious role of honey in the maintenance of TJ protein integrity, thereby preventing LPS-induced intestinal barrier disintegration.

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Bai Y, Huang F, Zhang R et al (2020) Longan pulp polysaccharides relieve intestinal injury in vivo and in vitro by promoting tight junction expression. Carbohydr Polym 229:115475. https://doi.org/10.1016/j.carbpol.2019.115475CrossRefPubMed

Yang Y, Li W, Sun Y et al (2015) Amino acid deprivation disrupts barrier function and induces protective autophagy in intestinal porcine epithelial cells. Amino Acids 47:2177–2184. https://doi.org/10.1007/s00726-014-1844-6CrossRefPubMed

Blikslager AT, Moeser AJ, Gookin JL et al (2007) Restoration of barrier function in injured intestinal mucosa. Physiol Rev 87:545–564. https://doi.org/10.1152/PHYSREV.00012.2006/ASSET/IMAGES/LARGE/Z9J0020724330008.JPEGCrossRefPubMed

Groschwitz KR, Hogan SP (2009) Intestinal barrier function: molecular regulation and disease pathogenesis. J Allergy Clin Immunol 124:3–20CrossRefPubMedPubMedCentral

Kurashima Y, Kiyono H (2017) Mucosal ecological network of epithelium and immune cells for gut homeostasis and tissue healing. Annu Rev Immunol 35:119–147CrossRefPubMed

Stokes CR (1995) Handbook of mucosal immunology. Immunol Today 16:115. https://doi.org/10.1016/0167-5699(95)80113-8CrossRef

Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM (1998) The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton. J Biol Chem 273:29745–29753. https://doi.org/10.1074/jbc.273.45.29745CrossRefPubMed

Furuse M, Hata M, Furuse K et al (2002) Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice. J Cell Biol 156:1099–1111. https://doi.org/10.1083/jcb.200110122CrossRefPubMedPubMedCentral

Clark JA, Doelle SM, Halpern MD et al (2006) Intestinal barrier failure during experimental necrotizing enterocolitis: protective effect of EGF treatment. Am J Physiol Gastrointest Liver Physiol. https://doi.org/10.1152/ajpgi.00090.2006CrossRefPubMed

10.

Camilleri M, Madsen K, Spiller R et al (2012) Intestinal barrier function in health and gastrointestinal disease. Neurogastroenterol Motil 24:503–512. https://doi.org/10.1111/J.1365-2982.2012.01921.XCrossRefPubMedPubMedCentral

11.

Piche T, Barbara G, Aubert P et al (2009) Impaired intestinal barrier integrity in the colon of patients with irritable bowel syndrome: involvement of soluble mediators. Gut 58:196–201. https://doi.org/10.1136/gut.2007.140806CrossRefPubMed

12.

Su L, Shen L, Clayburgh DR et al (2009) Targeted epithelial tight junction dysfunction causes immune activation and contributes to development of experimental colitis. Gastroenterology 136:551–563. https://doi.org/10.1053/j.gastro.2008.10.081CrossRefPubMed

13.

Williams JM, Duckworth CA, Watson AJM et al (2013) A mouse model of pathological small intestinal epithelial cell apoptosis and shedding induced by systemic administration of lipopolysaccharide. DMM Dis Model Mech 6:1388–1399. https://doi.org/10.1242/dmm.013284CrossRefPubMed

14.

Han X, Fink MP, Yang R, Delude RL (2004) Increased iNOS activity is essential for intestinal epithelial tight junction dysfunction in endotoxemic mice. Shock 21:261–270. https://doi.org/10.1097/01.shk.0000112346.38599.10CrossRefPubMed

15.

Adib-Conquy M, Cavaillon JM (2007) Stress molecules in sepsis and systemic inflammatory response syndrome. FEBS Lett 581:3723–3733. https://doi.org/10.1016/j.febslet.2007.03.074CrossRefPubMed

16.

Turner JR (2009) Intestinal mucosal barrier function in health and disease. Nat Rev Immunol 9:799–809CrossRefPubMed

17.

Musso G, Gambino R, Cassader M (2010) Gut microbiota as a regulator of energy homeostasis and ectopic fat deposition: mechanisms and implications for metabolic disorders. Curr Opin Lipidol 21:76–83. https://doi.org/10.1097/MOL.0b013e3283347ebbCrossRefPubMed

18.

Beutler B, Du X, Poltorak A (2016) Identification of Toll-like receptor 4 (Tlr4) as the sole conduit for LPS signal transduction: genetic and evolutionary studies. J Endotoxin Res 7:277–280. https://doi.org/10.1177/09680519010070040901CrossRef

19.

Beutler B (2002) Review paper: LPS in microbial pathogenesis: promise and fulfilment. J Endotoxin Res 8:329–335. https://doi.org/10.1177/09680519020080050601CrossRefPubMed

20.

Wellington MO, Hamonic K, Krone JEC et al (2020) Effect of dietary fiber and threonine content on intestinal barrier function in pigs challenged with either systemic E. coli lipopolysaccharide or enteric Salmonella typhimurium. J Anim Sci Biotechnol 11:1–12. https://doi.org/10.1186/s40104-020-00444-3CrossRef

21.

Bhattarai Y (2018) Microbiota-gut-brain axis: Interaction of gut microbes and their metabolites with host epithelial barriers. Neurogastroenterol Motil. https://doi.org/10.1111/NMO.13366CrossRefPubMed

22.

Roehlen N, Suarez AAR, El Saghire H et al (2020) Tight junction proteins and the biology of hepatobiliary disease. Int J Mol Sci. https://doi.org/10.3390/ijms21030825CrossRefPubMedPubMedCentral

23.

Meddings JB, Swain MG (2000) Environmental stress-induced gastrointestinal permeability is mediated by endogenous glucocorticoids in the rat. Gastroenterology 119:1019–1028. https://doi.org/10.1053/gast.2000.18152CrossRefPubMed

24.

Suzuki T, Hara H (2009) Quercetin enhances intestinal barrier function through the assembly of zonnula occludens-2, occludin, and claudin-1 and the expression of claudin-4 in caco-2 cells. J Nutr 139:965–974. https://doi.org/10.3945/jn.108.100867CrossRefPubMed

25.

Suzuki T, Tanabe S, Hara H (2011) Kaempferol enhances intestinal barrier function through the cytoskeletal association and expression of tight junction proteins in Caco-2 cells. J Nutr 141:87–94CrossRefPubMed

26.

Watson JL, Ansari S, Cameron H et al (2004) Green tea polyphenol (-)-epigallocatechin gallate blocks epithelial barrier dysfunction provoked by IFN-γ but not by IL-4. Am J Physiol Gastrointest Liver Physiol. https://doi.org/10.1152/ajpgi.00302.2003CrossRefPubMed

27.

Kim CY, Kim KH (2014) Curcumin prevents leptin-induced tight junction dysfunction in intestinal Caco-2 BBe cells. J Nutr Biochem 25:26–35. https://doi.org/10.1016/j.jnutbio.2013.08.011CrossRefPubMed

28.

Talebi M, Talebi M, Farkhondeh T, Samarghandian S (2020) Molecular mechanism-based therapeutic properties of honey. Biomed Pharmacother 130:110590CrossRefPubMed

29.

Cianciosi D, Forbes-Hernández TY, Afrin S et al (2018) Phenolic compounds in honey and their associated health benefits: a review. Molecules 23:2322CrossRefPubMedPubMedCentral

30.

Abubakar MB, Abdullah WZ, Sulaiman SA, Suen AB (2012) A review of molecular mechanisms of the anti-leukemic effects of phenolic compounds in honey. Int J Mol Sci 13:15054–15073CrossRefPubMedPubMedCentral

31.

Biesaga M, Pyrzynska K (2009) Liquid chromatography/tandem mass spectrometry studies of the phenolic compounds in honey. J Chromatogr A 1216:6620–6626. https://doi.org/10.1016/J.CHROMA.2009.07.066CrossRefPubMed

32.

Samarghandian S, Farkhondeh T, Samini F (2017) Honey and health: a review of recent clinical research. Pharmacognosy Res 9:121–127PubMedPubMedCentral

33.

Martinotti S, Ranzato E (2018) Honey, wound repair and regenerative medicine. J Funct Biomater 9:34CrossRefPubMedPubMedCentral

34.

Hadjmohammadi MR, Nazari SSSJ (2010) Separation optimization of quercetin, hesperetin and chrysin in honey by micellar liquid chromatography and experimental design. J Sep Sci 33:3144–3151. https://doi.org/10.1002/jssc.201000326CrossRefPubMed

35.

Tarnawski A (2005) Cellular and molecular mechanisms of gastrointestinal ulcer healing. Dig Dis Sci 50:24–33. https://doi.org/10.1007/s10620-005-2803-6CrossRef

36.

Nzeako BC, Al-Namaani F (2006) The antibacterial activity of honey on Helicobacter pylori. Sultan Qaboos Univ Med J 6:71–76PubMedPubMedCentral

37.

Ranzato E, Martinotti S (2014) Cellular and molecular mechanisms of honey wound healing. Nova Science Publishers, Hauppauge, NY, pp 1–155

38.

Van De Walle J, Hendrickx A, Romier B et al (2010) Inflammatory parameters in Caco-2 cells: effect of stimuli nature, concentration, combination and cell differentiation. Toxicol Vitr 24:1441–1449. https://doi.org/10.1016/j.tiv.2010.04.002CrossRef

39.

Hubatsch I, Ragnarsson EGE, Artursson P (2007) Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers. Nat Protoc 2:2111–2119. https://doi.org/10.1038/nprot.2007.303CrossRefPubMed

40.

Shao Y, Wolf PG, Guo S et al (2017) Zinc enhances intestinal epithelial barrier function through the PI3K/AKT/mTOR signaling pathway in Caco-2 cells. J Nutr Biochem 43:18–26. https://doi.org/10.1016/j.jnutbio.2017.01.013CrossRefPubMed

41.

Putt KK, Pei R, White HM, Bolling BW (2017) Yogurt inhibits intestinal barrier dysfunction in Caco-2 cells by increasing tight junctions. Food Funct 8:406–414. https://doi.org/10.1039/c6fo01592aCrossRefPubMed

42.

Ling X, Linglong P, Weixia D, Hong W (2016) Protective effects of bifidobacterium on intestinal barrier function in LPS-induced enterocyte barrier injury of Caco-2 monolayers and in a rat NEC model. PLoS ONE. https://doi.org/10.1371/journal.pone.0161635CrossRefPubMedPubMedCentral

43.

Afrin S, Giampieri F, Gasparrini M et al (2018) The inhibitory effect of Manuka honey on human colon cancer HCT-116 and LoVo cell growth. Part 1: the suppression of cell proliferation, promotion of apoptosis and arrest of the cell cycle. Food Funct 9:2145–2157. https://doi.org/10.1039/C8FO00164BCrossRefPubMed

44.

Gasparrini M, Afrin S, Forbes-Hernández TY et al (2018) Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages. Part 2: control of oxidative stress induced damage, increase of antioxidant enzyme activities and attenuation of inflammation. Food Chem Toxicol 120:578–587. https://doi.org/10.1016/J.FCT.2018.08.001CrossRefPubMed

45.

Jaganathan SK (2009) Honey constituents and their apoptotic effect in colon cancer cells. J ApiProduct ApiMedical Sci 1:29–36. https://doi.org/10.3896/ibra.4.01.2.02CrossRef

46.

Ruiz-Matute AI, Soria AC, Martínez-Castro I, Sanz ML (2007) A new methodology based on GC-MS to detect honey adulteration with commercial syrups. J Agric Food Chem 55:7264–7269. https://doi.org/10.1021/jf070559jCrossRefPubMed

47.

Ghaffarian R, Muro S (2013) Models and methods to evaluate transport of drug delivery systems across cellular barriers. J Vis Exp. https://doi.org/10.3791/50638CrossRefPubMedPubMedCentral

48.

Ranneh Y, Akim AM, Hamid HA et al (2021) Honey and its nutritional and anti-inflammatory value. BMC Complement Med Ther 21:1–17. https://doi.org/10.1186/s12906-020-03170-5CrossRef

49.

Gencay C, Kilicoglu SS, Kismet K et al (2008) Effect of honey on bacterial translocation and intestinal morphology in obstructive jaundice. World J Gastroenterol 14:3410–3415. https://doi.org/10.3748/wjg.14.3410CrossRefPubMedPubMedCentral

50.

Mehranfard N, Yazdi A, Rafiei A et al (2020) Honey protects against chronic unpredictable mild stress induced- intestinal barrier disintegration and hepatic inflammation. Mol Biol Rep 47:8475–8484. https://doi.org/10.1007/s11033-020-05888-4CrossRefPubMed

51.

Cai G, Wu Y, Wusiman A et al (2021) Alhagi honey polysaccharides attenuate intestinal injury and immune suppression in cyclophosphamide-induced mice. Food Funct 12:6863–6877. https://doi.org/10.1039/d1fo01008eCrossRefPubMed

52.

Almasaudi SB, Abbas AT, Al-Hindi RR et al (2017) Manuka honey exerts antioxidant and anti-inflammatory activities that promote healing of acetic acid-induced gastric ulcer in rats. Evid Based Complement Alternat Med. https://doi.org/10.1155/2017/5413917CrossRefPubMedPubMedCentral

53.

Kassim M, Yusoff KM, Ong G et al (2012) Gelam honey inhibits lipopolysaccharide-induced endotoxemia in rats through the induction of heme oxygenase-1 and the inhibition of cytokines, nitric oxide, and high-mobility group protein B1. Fitoterapia 83:1054–1059. https://doi.org/10.1016/J.FITOTE.2012.05.008CrossRefPubMed

54.

Cani PD, Bibiloni R, Knauf C et al (2008) Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet–induced obesity and diabetes in mice. Am Diabetes Assoc 57:1470–1481. https://doi.org/10.2337/db07-1403CrossRef

55.

Woting A, Blaut M (2018) Small intestinal permeability and gut-transit time determined with low and high molecular weight fluorescein isothiocyanate-dextrans in C3H mice. Nutrients. https://doi.org/10.3390/nu10060685CrossRefPubMedPubMedCentral

56.

Feldman AT, Wolfe D (2014) Tissue processing and hematoxylin and eosin staining. Methods Mol Biol 1180:31–43. https://doi.org/10.1007/978-1-4939-1050-2_3CrossRefPubMed

57.

Erben U, Christoph Loddenkemper KD, Spieckermann S et al (2014) A guide to histomorphological evaluation of intestinal inflammation in mouse models. Int J Clin Exp Pathol 7:4557–4576PubMedPubMedCentral

58.

Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3:1101–1108. https://doi.org/10.1038/nprot.2008.73CrossRefPubMed

59.

Huang L, Cui K, Mao W et al (2020) Weissella cibaria attenuated LPS-induced dysfunction of intestinal epithelial barrier in a Caco-2 cell monolayer model. Front Microbiol. https://doi.org/10.3389/fmicb.2020.02039CrossRefPubMedPubMedCentral

60.

Pereira da Costa M, Conte-Junior CA (2015) Chromatographic methods for the determination of carbohydrates and organic acids in foods of animal origin. Compr Rev Food Sci Food Saf 14:586–600. https://doi.org/10.1111/1541-4337.12148CrossRef

61.

Puścion-Jakubik A, Borawska MH, Socha K (2020) Modern methods for assessing the quality of Bee Honey and botanical origin identification. Foods 9:1028. https://doi.org/10.3390/foods9081028CrossRefPubMedPubMedCentral

62.

Gómez-Caravaca AM, Gómez-Romero M, Arráez-Román D et al (2006) Advances in the analysis of phenolic compounds in products derived from bees. J Pharm Biomed Anal 41:1220–1234. https://doi.org/10.1016/j.jpba.2006.03.002CrossRefPubMed

63.

Chen J, Zhang R, Wang J et al (2015) Protective effects of baicalin on LPS-induced injury in intestinal epithelial cells and intercellular tight junctions. Can J Physiol Pharmacol 93:233–237. https://doi.org/10.1139/cjpp-2014-0262CrossRefPubMed

64.

Kordulewska NK, Topa J, Tańska M et al (2021) Modulatory effects of osthole on lipopolysaccharides-induced inflammation in Caco-2 cell monolayer and co-cultures with THP-1 and THP-1-derived macrophages. Nutrients 13:1–16. https://doi.org/10.3390/nu13010123CrossRef

65.

Kim MS, Kim JY (2017) Intestinal anti-inflammatory effects of cinnamon extracts in a co-culture model of intestinal epithelial Caco-2 cells and RAW264.7 macrophages. Appl Biol Chem 60:553–561. https://doi.org/10.1007/s13765-017-0311-yCrossRef

66.

Zhao H, Zhang H, Wu H et al (2012) Protective role of 1,25(OH)2vitamin D3 in the mucosal injury and epithelial barrier disruption in DSS-induced acute colitis in mice. BMC Gastroenterol 12:1–14. https://doi.org/10.1186/1471-230X-12-57CrossRefPubMedPubMedCentral

67.

Guo S, Al-Sadi R, Said HM, Ma TY (2013) Lipopolysaccharide causes an increase in intestinal tight junction permeability in vitro and in vivo by inducing enterocyte membrane expression and localization of TLR-4 and CD14. Am J Pathol 182:375–387. https://doi.org/10.1016/j.ajpath.2012.10.014CrossRefPubMedPubMedCentral

68.

König J, Wells J, Cani PD et al (2016) Human intestinal barrier function in health and disease. Clin Transl Gastroenterol. https://doi.org/10.1038/ctg.2016.54CrossRefPubMedPubMedCentral

69.

Vancamelbeke M, Vermeire S (2017) The intestinal barrier: a fundamental role in health and disease. Expert Rev Gastroenterol Hepatol 11:821–834. https://doi.org/10.1080/17474124.2017.1343143CrossRefPubMedPubMedCentral

70.

De Santis S, Cavalcanti E, Mastronardi M et al (2015) Nutritional keys for intestinal barrier modulation. Front Immunol. https://doi.org/10.3389/fimmu.2015.00612CrossRefPubMedPubMedCentral

71.

Ménard S, Cerf-Bensussan N, Heyman M (2010) Multiple facets of intestinal permeability and epithelial handling of dietary antigens. Mucosal Immunol 3:247–259. https://doi.org/10.1038/mi.2010.5CrossRefPubMed

72.

He S, Guo Y, Zhao J et al (2020) Ferulic acid ameliorates lipopolysaccharide-induced barrier dysfunction via MicroRNA-200c-3p-mediated activation of PI3K/AKT pathway in Caco-2 cells. Front Pharmacol 11:1–13. https://doi.org/10.3389/fphar.2020.00376CrossRef

73.

Xu J, Song J, Zhang Y et al (2020) Jinzhi protects lipopolysaccharide-treated mice against mortality by repairing intestinal mucosal barrier damage and intestinal microecology. Biomed Pharmacother. https://doi.org/10.1016/j.biopha.2019.109749CrossRefPubMedPubMedCentral

74.

Beguin P, Errachid A, Larondelle Y, Schneider YJ (2013) Effect of polyunsaturated fatty acids on tight junctions in a model of the human intestinal epithelium under normal and inflammatory conditions. Food Funct 4:923–931. https://doi.org/10.1039/c3fo60036jCrossRefPubMed

75.

Ghosh SS, Wang J, Yannie PJ, Ghosh S (2020) Intestinal barrier dysfunction, LPS translocation, and disease development. J Endocr Soc 4:1–15. https://doi.org/10.1210/jendso/bvz039CrossRef

76.

Catalioto R-M, AMaggiGiuliani ACS (2012) Intestinal epithelial barrier dysfunction in disease and possible therapeutical interventions. Curr Med Chem 18:398–426. https://doi.org/10.2174/092986711794839179CrossRef

77.

Raynaud A, Ghezali L, Gloaguen V et al (2013) Honey-induced macrophage stimulation: AP-1 and NF-κB activation and cytokine production are unrelated to LPS content of honey. Int Immunopharmacol 17:874–879. https://doi.org/10.1016/j.intimp.2013.09.014CrossRefPubMed

78.

Wu L, Du B, Vander Heyden Y et al (2017) Recent advancements in detecting sugar-based adulterants in honey—a challenge. TrAC Trends Anal Chem 86:25–38. https://doi.org/10.1016/j.trac.2016.10.013CrossRef

79.

Erejuwa OO, Sulaiman SA, Wahab MSA (2012) molecules fructose might contribute to the hypoglycemic effect of honey. Molecules. https://doi.org/10.3390/molecules17021900CrossRefPubMedPubMedCentral

80.

Walle T, Otake Y, Brubaker JA et al (2001) Disposition and metabolism of the flavonoid chrysin in normal volunteers. Br J Clin Pharmacol 51:143–146. https://doi.org/10.1111/j.1365-2125.2001.01317.xCrossRefPubMedPubMedCentral

81.

Roberts AT, Martin CK, Liu Z et al (2007) The safety and efficacy of a dietary herbal supplement and gallic acid for weight loss. J Med Food 10:184–188. https://doi.org/10.1089/jmf.2006.272CrossRefPubMed

82.

Cao G, Ying P, Yan B et al (2015) Pharmacokinetics, safety, and tolerability of single and multiple-doses of pinocembrin injection administered intravenously in healthy subjects. J Ethnopharmacol 168:31–36. https://doi.org/10.1016/j.jep.2015.03.041CrossRefPubMed

83.

dos Scholz MBS, Quinhone Júnior A, Delamuta BH et al (2020) Indication of the geographical origin of honey using its physicochemical characteristics and multivariate analysis. J Food Sci Technol 57:1896. https://doi.org/10.1007/S13197-019-04225-3CrossRefPubMedPubMedCentral

84.

El Sohaimy SA, Masry SHD, Shehata MG (2015) Physicochemical characteristics of honey from different origins. Ann Agric Sci 60:279–287. https://doi.org/10.1016/J.AOAS.2015.10.015CrossRef

85.

Wolfs TGAM, Kramer BW, Thuijls G et al (2014) Chorioamnionitis-induced fetal gut injury is mediated by direct gut exposure of inflammatory mediators or by lung inflammation. Am J Physiol Gastrointest Liver Physiol 306:382–393. https://doi.org/10.1152/ajpgi.00260.2013CrossRef

86.

Wang X, Wang S, Li Y et al (2013) Sulfated Astragalus polysaccharide can regulate the inflammatory reaction induced by LPS in Caco2 cells. Int J Biol Macromol 60:248–252. https://doi.org/10.1016/j.ijbiomac.2013.05.037CrossRefPubMed

87.

Soufli I, Toumi R, Rafa H, Touil-Boukoffa C (2016) Overview of cytokines and nitric oxide involvement in immuno-pathogenesis ofinflammatory bowel diseases. World J Gastrointest Pharmacol Ther 7:353. https://doi.org/10.4292/WJGPT.V7.I3.353CrossRefPubMedPubMedCentral

88.

Dörfel MJ, Huber O (2012) Modulation of tight junction structure and function by kinases and phosphatases targeting occludin. J Biomed Biotechnol 2012:807356. https://doi.org/10.1155/2012/807356CrossRefPubMedPubMedCentral

89.

Wei L, Li Y, Tang W et al (2019) Chronic unpredictable mild stress in rats induces colonic inflammation. Front Physiol. https://doi.org/10.3389/fphys.2019.01228CrossRefPubMedPubMedCentral

90.

Moreira APB, Texeira TFS, Ferreira AB et al (2012) Influence of a high-fat diet on gut microbiota, intestinal permeability and metabolic endotoxaemia. Br J Nutr 108:801–809. https://doi.org/10.1017/S0007114512001213CrossRefPubMed

91.

Oguz S, Salt O, Ibis AC et al (2018) Combined effectiveness of honey and immunonutrition on bacterial translocation secondary to obstructive jaundice in rats: experimental study. Med Sci Monit 24:3374–3381. https://doi.org/10.12659/MSM.907977CrossRefPubMedPubMedCentral

92.

Miguel MG, Antunes MD, Faleiro ML (2017) Honey as a complementary medicine. Integr Med Insights 12:1–15. https://doi.org/10.1177/1178633717702869CrossRef

93.

Ajibola A, Chamunorwa JP, Erlwanger KH (2012) Nutraceutical values of natural honey and its contribution to human health and wealth. Nutr Metab 9:1–12. https://doi.org/10.1186/1743-7075-9-61CrossRef

94.

Nair A, Jacob S (2016) A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm 7:27. https://doi.org/10.4103/0976-0105.177703CrossRefPubMedPubMedCentral

95.

Gupta J, Nebreda A (2014) Analysis of intestinal permeability in mice. Bio-Protoc. https://doi.org/10.21769/bioprotoc.1289CrossRef

96.

Cho YE, Kim DK, Seo W et al (2021) Fructose promotes leaky gut, endotoxemia, and liver fibrosis through ethanol-inducible cytochrome P450–2E1–mediated oxidative and nitrative stress. Hepatology 73:2180–2195. https://doi.org/10.1002/hep.30652CrossRefPubMed

Titel: Consumption of honey ameliorates lipopolysaccharide-induced intestinal barrier dysfunction via upregulation of tight junction proteins
verfasst von: Sandip Nathani
Neeladrisingha Das
Parul Katiyar
Bhairavnath Waghmode
Debabrata Sircar
Partha Roy
Publikationsdatum: 26.07.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 7/2023
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-023-03203-y

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