Background
Methods
Organisms and chemicals
Strains | Antimicrobial susceptibility | |||||
---|---|---|---|---|---|---|
Gentamycin (µg/ml) | Erythromycin (µg/ml) | Thymoquinone (µg/ml) | ||||
aMIC |
bMBC | MIC | MBC | MIC | MBC | |
Gram negative bacilli | ||||||
Escherichi coli ATCC 35218 | 8 | 16 | 32 | 64 | >512 | >512 |
Pseudomonas aeruginosa ATCC 27853 | 2 | 4 | 256 | >256 | >512 | >512 |
Salmonella enterica serovar Typhimurium ATCC 14028 | 2 | 8 | >256 | >256 | >512 | >512 |
Vibrio alginolyticus ATCC 33787 | 32 | 64 | >256 | >256 | 256 | >512 |
Vibrio paraheamolyticus ATCC 17802 | 8 | 16 | 128 | 256 | 32 | 64 |
Gram positive bacilli | ||||||
Bacillus cereus ATCC 14579 | 4 | 8 | 8 | 16 | 8 | 8 |
Listeria monocytogene ATCC 19115 | 2 | 4 | 1 | 4 | 16 | 32 |
Gram positive cocci | ||||||
Enterococcus faecalis ATCC 29212 | 32 | 64 | 256 | >256 | 32 | 64 |
Micrococcus luteus NCIMB 8166 | 2 | 8 | 4 | 16 | 8 | 64 |
Staphylococcus aureus ATCC 25923 | 16 | 32 | 16 | 32 | 8 | 16 |
Staphylococcus epidermidis CIP 106510 | 4 | 8 | 16 | 32 | 8 | 8 |
Minimum inhibitory concentration determination
Minimum bactericidal concentration determination
Effect of Thymoquinone on biofilm formation
Crystal Violet assay
Strains | Inhibition of biofilm development (%) | |||
---|---|---|---|---|
Crystal Violet assay | XTT assay | |||
aBIC50 (µg/ml) |
bBIC90 (µg/ml) | BIC50 (µg/ml) | BIC90 (µg/ml) | |
Enterococcus faecalis ATCC 29212 | 85 | 349 | 44 | 145 |
Staphylococcus aureus ATCC 25923 | 22 | 75 | 20.5 | 51 |
Staphylococcus epidermidis CIP 106510 | 60 | 109 | 40 | 90 |
Pseudomonas aeruginosa ATCC 27853 | >512 | >512 | >512 | >512 |