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European Journal of Nutrition

Erschienen in:

23.04.2020 | Original Contribution

Protective effects of bovine milk exosomes against oxidative stress in IEC-6 cells

verfasst von: Lanfang Wang, Zhexi Shi, Xinyan Wang, Shu Mu, Xiaoyan Xu, Li Shen, Ping Li

Erschienen in: European Journal of Nutrition | Ausgabe 1/2021

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Abstract

Purpose

Bovine milk exosomes, which are enriched with microRNAs (miRNAs) and proteins, regulate immune response and growth. In the present study, we aimed to assess the protective effects of bovine milk exosomes against oxidative stress of intestinal crypt epithelial cells (IEC-6).

Methods

Bovine milk exosomes were isolated and characterized. To assess the protective effects of exosomes, IEC-6 cells were pretreated with exosomes, followed by H₂O₂. Cell viability and levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPX), reactive oxidative species (ROS), and lactate dehydrogenase (LDH) were measured. The expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (Ho1) genes, and miR-146a, miR-155, and the HO-1 protein were also determined.

Results

The isolated bovine milk exosome were positive for CD63 and CD9 expression. The exosomes were approximately circular and had a diameter of about 67.23 nm. Pretreatment of IEC-6 cells with bovine milk exosomes enhanced cell viability; increased SOD and GPX activities; and reduced LDH, ROS, and MDA levels after H₂O₂ challenge. Further analysis showed that exosome pretreatment increased intracellular miR-146a and miR-155 levels. Exosome pretreatment inhibited the elevation of Nrf2 and Ho1 gene expression induced by H₂O₂, but promoted HO-1 protein expression.

Conclusion

The results indicated that bovine milk exosomes exerted protective effects against oxidative stress in IEC-6 cells.

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Titel: Protective effects of bovine milk exosomes against oxidative stress in IEC-6 cells
verfasst von: Lanfang Wang
Zhexi Shi
Xinyan Wang
Shu Mu
Xiaoyan Xu
Li Shen
Ping Li
Publikationsdatum: 23.04.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 1/2021
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-020-02242-z

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Protective effects of bovine milk exosomes against oxidative stress in IEC-6 cells

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Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

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Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

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GLP-1-Agonist Semaglutid wirkt kardio- und nephroprotektiv

Bei seelischem Stress sind Checkpoint-Hemmer weniger wirksam

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