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Strahlentherapie und Onkologie

Erschienen in:

01.01.2014 | Original article

The effect of resveratrol in combination with irradiation and chemotherapy

Study using Merkel cell carcinoma cell lines

verfasst von: G. Heiduschka, C. Lill, R. Seemann, M. Brunner, R. Schmid, R. Houben, J. Bigenzahn, D. Thurnher, M.D.

Erschienen in: Strahlentherapie und Onkologie | Ausgabe 1/2014

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Abstract

Background and purpose

Merkel cell carcinoma (MCC) is a rare, but highly malignant tumor of the skin. In case of systemic disease, possible therapeutic options include irradiation or chemotherapy. The aim of this study was to evaluate whether the flavonoid resveratrol enhances the effect of radiotherapy or chemotherapy in MCC cell lines.

Materials and methods

The two MCC cell lines MCC13 and MCC26 were treated with increasing doses of resveratrol. Combination experiments were conducted with cisplatin and etoposide. Colony forming assays were performed after sequential irradiation with 1, 2, 3, 4, 6, and 8 Gy and apoptosis was assessed with flow cytometry. Expression of cancer drug targets was analyzed by real-time PCR array.

Results

Resveratrol is cytotoxic in MCC cell lines. Cell growth is inhibited by induction of apoptosis. The combination with cisplatin and etoposide resulted in a partially synergistic inhibition of cell proliferation. Resveratrol and irradiation led to a synergistic reduction in colony formation compared to irradiation alone. Evaluation of gene expression did not show significant difference between the cell lines.

Conclusion

Due to its radiosensitizing effect, resveratrol seems to be a promising agent in combination with radiation therapy. The amount of chemosensitizing depends on the cell lines tested.

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Titel: The effect of resveratrol in combination with irradiation and chemotherapy
Study using Merkel cell carcinoma cell lines
verfasst von: G. Heiduschka
C. Lill
R. Seemann
M. Brunner
R. Schmid
R. Houben
J. Bigenzahn
D. Thurnher, M.D.
Publikationsdatum: 01.01.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Strahlentherapie und Onkologie / Ausgabe 1/2014
Print ISSN: 0179-7158
Elektronische ISSN: 1439-099X
DOI: https://doi.org/10.1007/s00066-013-0445-8

Springer Medizin

The effect of resveratrol in combination with irradiation and chemotherapy

Study using Merkel cell carcinoma cell lines