Plain Summary
Background
Methods
Study design
Ethical approval
Participants
Sample size calculation
Sampling technique and data collection
Body mass index
Outcomes
Semen standard parameters analysis
Sperm chromatin dispersion assay (SCD)
Toluidine blue (TB) staining
Chromomycin A3 staining
Ovarian stimulation, IVF, ICSI, and embryo development
Data analysis
Results
Demographic parameters
Normal weight (BMI < 24.5 kg/m2) (347) | Over weight (BMI ≥ 24.5 kg/m2) (403) | Whole studied population (750) | p-Value | |
---|---|---|---|---|
Paternal Age (Years) | 38.7 ± 0.26 | 38.9 ± 0.24 | 38.8 ± 0.25 | 0.68 |
Weight (KG) | 72.5 ± 0.4 | 84.07 ± 0.3** | 77.8 ± 0.4 | < 0.001 |
Height (cm) | 75.49 ± 4.3 | 95.06 ± 5.07** | 86.93 ± 3.31 | < 0.001 |
Paternal BMI(Kg/m2) | 22.3 ± 0.15 | 27.6 ± 0.3** | 25.6 ± 0.1 | < 0.001 |
Married for (Years) | 9.1 ± 0.8 | 9.4 ± 0.6 | 9.10 ± 0.7 | 0.52 |
Maternal age (Years) | 33.1 ± 0.27 | 33.1 ± 0.29 | 32.9 ± 0.28 | 0.33 |
Maternal BMI(Kg/m2) SEMEN PARAMETERS | ||||
Liquefaction time (Minutes) | 31.75 ± 0.71 | 31.98 ± 0.46 | 31.56 ± 0.39 | 0.06 |
Volume (ml) | 3.85 ± 0.09 | 3.81 ± 0.1 | 3.69 ± 0.06 | 0.39 |
PH | 8 ± 0.0 | 7.9 ± 0.0 | 7.97 ± 0.01 | 0.27 |
WBC/HPF | 4.65 ± 0.32 | 4.35 ± 0.56 | 4.52 ± 0.29 | 0.25 |
Paternal BMI and semen standard parameters
Sperm Parameters | Normal weight (BMI < 24.5 kg/m2) (347) | Over weight (BMI ≥ 24.5 kg/m2) (403) | Whole studied population (750) | p-Value |
---|---|---|---|---|
Concentration × 106 | 62.89 ± 3.30 | 61.47 ± 2.22 | 62.01 ± 2.23 | 0.83 |
Morphology Normal | 3.6 ± 0.99 | 3.5 ± 0.85 | 3.5 ± 0.65 | 0.24 |
TZI | 1.81 ± 0.3 | 1.82 ± 0.3 | 1.81 ± 0.2 | 0.67 |
SDI | 1.13 ± 0.3 | 1.19 ± 0.3 | 1.17 ± 0.2 | 0.56 |
TNMS | 11.44 ± 1.5 | 9.32 ± 0.9 | 10.75 ± 0.9 | 0.002 |
ROS | 1.68 ± 0.6 | 1.9 ± 0.6 | 1.79 ± 0.4 | < 0.001 |
HOS | 53.9 ± 0.9 | 53.3 ± 0.8 | 53.7 ± 0.9 | 0.81 |
DFI-SCD | 22.91 ± 1.23 | 28.74 ± 1.04 | 25.38 ± 3.80 | < 0.001 |
Sperm chromatin integrity parameters
Peri-fertilization stage
Parameters | normal weight (BMI < 24.5 kg/m2) (347) | Overweight (BMI ≥ 24.5 kg/m2) (403) | Whole studied population (750) | P-Value |
---|---|---|---|---|
TEC | 8.9 ± 6.4 (2471) | 9.2 ± 7.0 (3942) | 9.1 ± 6.7 (6413) | 0.59 |
MII | 7.7 ± 0.5 (2188) | 7.8 ± 0.6 (3292) | 7.7 ± 0.6 (5480) | 0.83 |
2PN | 5.3 ± 0.7 (1501) | 5.2 ± 0.2 (2091) | 5.2 ± 0.4 (3592) | 0.84 |
FR | 74.8 ± 0.25 | 67 ± 0.2 | 70.2 ± 0.8 | 0.001 |
D3 embryos | 3.8 ± 0.7 | 4.1 ± 0.4 | 4.0 ± 0.2 | 0.31 |
EGA | 2.8 ± 0.9 | 2.7 ± 0.4 | 2.7 ± 0.6 | 0.89 |
CR | 82.4 ± 0.24 | 83.5 ± 0.23 | 83.1 ± 0.24 | 0.56 |
BR | 42.5 ± 0.24 | 39 ± 0.25 | 40.7 ± 0.24 | 0.42 |
Aneuploidy (%) | 39.1 ± 2.1 | 38.1 ± 2.0 | 38.1 ± 1.4 | 0.45 |
IR (%) | 131/347 (37%) | 143/403 (35%) | 274/750 (36%) | 0.51 |
CLBR | 31.2 ± 3.4 | 20.1 ± 3.2 | 25.2 ± 3.4 | 0.02 |
Birth weight (gm) | 2577.24 ± 30.94 | 2952.14 ± 53.64 | 2752.5 ± 36.98 | < 0.001 |
PBMI | CMA3 | TB | DFI-SCD | HOS | ROS | ||
---|---|---|---|---|---|---|---|
Paternal BMI kg/m2 | r = | 0.79** | 0.114* | 0.282** | 0.012 | 0.282** | |
p = | < 0.001 | .030 | < 0.001 | 0.742 | < 0.001 | ||
FR | r = | -0.187** | -0.043 | -0.031 | -0.062 | 0.070 | -0.148** |
p = | < 0.001 | 0.261 | 0.422 | 0.107 | .068 | < 0.001 | |
D3 Embr | r | -0.019 | 0.075 | -0.049 | -0.038 | 0.051 | 0.021 |
p | 0.70 | 0.593 | 0.13 | 0.45 | 0.301 | 0.59 | |
EGA | r = | 0.052 | 0.08 | 0.021 | 0.030 | -0.01 | 0.06 |
p = | 0.325 | 0.1 | 0.693 | 0.577 | 0.78 | 0.259 | |
CR | r = | -0.110** | .009 | .024 | -0.005 | -0.023 | 0.042 |
p = | 0.004 | 0.814 | 0.526 | 0.897 | 0.556 | 0.277 | |
BR | r = | 0.01 | 0.07 | 0.08 | 0.09 | 0.23 | -0.039 |
p = | 0.96 | 0.59 | 0.64 | 0.49 | 0.15 | 0.7 | |
Euploid | r = | 0.061 | 0.060 | -0.014 | 0.007 | -0.004 | 0.045 |
p = | 0.142 | 0.143 | 0.740 | 0.858 | 0.923 | 0.279 | |
ET | r = | -0.052 | 0.011 | 0.015 | -0.003 | 0.105** | 0.005 |
p = | 0.157 | 0.763 | 0.684 | 0.939 | 0.004 | 0.882 | |
IR | r = | 0.42 | 0.008 | 0.05 | 0.027 | 0.016 | -0.062 |
p = | 0.25 | 0.83 | 0.17 | 0.45 | 0.654 | 0.09 | |
CLBR | r = | -0.38** | 0.002 | 0.025 | -0.09 | -0.03 | -0.52 |
p = | < 0.001 | 0.445 | 0.97 | 0.16 | 0.59 | 0.44 |