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European Journal of Nutrition

Erschienen in:

01.12.2012 | Original Contribution

Effect of docosahexaenoic acid on hypoxia/reoxygenation injury in human coronary arterial smooth muscle cells

verfasst von: Guan-Ming Feng, Jia-Huei Chen, Cheng-I Lin, Jung-Mou Yang

Erschienen in: European Journal of Nutrition | Ausgabe 8/2012

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Abstract

Purpose

Hypoxia and reoxygenation (H/R) occur in a wide variety of important clinical conditions such as myocardial infarction. H/R injury is a complex phenomenon involving not only intracellular damage processes but also an injurious inflammatory response. Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid, has long been proved to be protective against several types of cardiovascular disease. However, its beneficial effect during H/R is inconclusive. In this study, we employed an in vitro model to examine whether DHA is protective against H/R-induced cell damage in human coronary artery smooth muscle cells (HCASMCs).

Methods

HCASMCs in the absence or presence of DHA (1, 3, 10, and 30 μM) were subjected to control or H/R treatment using a modular incubator chamber to create hypoxic condition. Cell viability was evaluated by MTT assay. Spectrophotometric and spectrofluorometric assays were used to measure the generation of nitric oxide (NO) and reactive oxygen species (ROS), respectively. Inflammatory cytokines were determined by enzyme-linked immunosorbent assay. Intracellular calcium mobilization was estimated microfluorimetrically using calcium indicator dye, fura 2-acetomethyl ester.

Results

Hypoxia/reoxygenation caused significant injury in cultured HCASMCs. DHA at low concentrations (1, 3, and 10 μM) did not afford protection, whereas at 30 μM, it caused deleterious effects, presumably by enhancing the production of NO, ROS, IL-1β, and IL-6 and altering the intracellular calcium dynamics.

Conclusions

Our results do not support the protective function of DHA in H/R-injured coronary arterial smooth muscle cells.

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Titel: Effect of docosahexaenoic acid on hypoxia/reoxygenation injury in human coronary arterial smooth muscle cells
verfasst von: Guan-Ming Feng
Jia-Huei Chen
Cheng-I Lin
Jung-Mou Yang
Publikationsdatum: 01.12.2012
Verlag: Springer-Verlag
Erschienen in: European Journal of Nutrition / Ausgabe 8/2012
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-011-0278-0

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