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04.05.2022 | Original Contribution

Improved microvascular reactivity after aged garlic extract intake is not mediated by hydrogen sulfide in older adults at risk for cardiovascular disease: a randomized clinical trial

verfasst von: Renata Leitão, Gustavo Vieira de Oliveira, Cristina Rezende, Mônica Volino-Souza, Jacilene Mesquita, Leandro Lara de Carvalho, Thiago Silveira Alvares

Erschienen in: European Journal of Nutrition | Ausgabe 7/2022

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Abstract

Purpose

This study aimed to investigate the effects of AGE on microvascular reactivity, systolic blood pressure (SBP), and diastolic blood pressure (DBP) in older individuals at high risk for cardiovascular disease (CVD). Urinary thiosulfate was also investigated as an indirect marker of endogenous hydrogen sulfide (H₂S) synthesis. The study was conducted in a randomized, double-blind, crossover, and placebo-controlled way.

Methods

Twenty-eight participants (14 male), 67 ± 6 years old with CVD risk factors, ingested 2.4 g of AGE or placebo (PLA). Near-infrared spectroscopy evaluated tissue oxygen saturation (StO₂) during a vascular occlusion test (30 s baseline, 5 min occlusion, and 2 min reperfusion). The upslope of StO₂ signal after cuff release was calculated to measure microvascular reactivity. Urinary thiosulfate levels were measured using a high-performance liquid chromatography system.

Results

The upslope of StO₂ was significantly faster after AGE (1.01 ± 0.37% s⁻¹) intake compared to PLA (0.83 ± 0.35% s⁻¹; P < 0.001; d = 0.50). Relative changes in Δ% SBP from pre- to post-AGE intake (− 5.17 ± 5.77%) was significantly different compared to Δ% PLA (0.32 ± 5.99%; P = 0.001; d = 0.93). No significant changes in urinary thiosulfate concentrations were observed between interventions. Moreover, no significant gender effect in any parameter assessed was found.

Conclusion

This study demonstrated that a single dose of AGE improved microvascular reactivity in older adults at risk of CVD despite such an effect was not linked with urinary thiosulfate levels. This trial was registered at clinicaltrials.gov as NCT04008693 (May 19, 2020).

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Titel: Improved microvascular reactivity after aged garlic extract intake is not mediated by hydrogen sulfide in older adults at risk for cardiovascular disease: a randomized clinical trial
verfasst von: Renata Leitão
Gustavo Vieira de Oliveira
Cristina Rezende
Mônica Volino-Souza
Jacilene Mesquita
Leandro Lara de Carvalho
Thiago Silveira Alvares
Publikationsdatum: 04.05.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 7/2022
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-022-02895-y

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Improved microvascular reactivity after aged garlic extract intake is not mediated by hydrogen sulfide in older adults at risk for cardiovascular disease: a randomized clinical trial