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Cardiovascular Toxicology
Erschienen in: Cardiovascular Toxicology 2/2012

01.06.2012

Grape Seed and Skin Extract Protects Against Acute Chemotherapy Toxicity induced by Doxorubicin in Rat Heart

verfasst von: Meherzia Mokni, Sonia Hamlaoui-Guesmi, Mohamed Amri, Lamjed Marzouki, Ferid Limam, Ezzedine Aouani

Erschienen in: Cardiovascular Toxicology | Ausgabe 2/2012

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Abstract

Doxorubicin (Dox), an antitumor anthracycline antibiotic, plays a key role in the treatment of many neoplastic diseases. However, its chronic administration induces cardiomyopathy. Increased oxidative stress is a major factor implicated in Dox-induced cardiotoxicity. We hypothesized that a pre-treatment with grape seed and skin extract (GSE), commonly used as an antioxidant agent, may alleviate this cardiotoxicity. Rats were treated with GSE (500 mg/kg bw) by intraperitoneal injection during 8 days. On the 4th day, rats were administered a single dose of Dox (20 mg/kg). At the end of the treatment, their hearts were Langendorff-perfused, subjected to ischemia/reperfusion (I/R) injury, and left ventricular functions as heart rate and developed pressure measured. Hearts were also used to determine free iron, H2O2, Ca2+, lipoperoxidation, carbonylation and antioxidant enzymes such as superoxide dismutase (SOD), catalase and peroxidase. Doxorubicin drastically affected heart activity as evidenced after I/R experiments. This effect was associated with an increase in heart free iron and a decrease in Ca2+ concentrations. This effect may have contributed to oxidative stress as assessed by high lipoperoxidation and carbonylation level. GSE counteracted Dox-induced disturbances of hemodynamic parameters, alleviated oxidative stress as assessed by normalized iron and Ca2+ levels and increased SOD activity especially the Mn isoform.
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Metadaten
Titel
Grape Seed and Skin Extract Protects Against Acute Chemotherapy Toxicity induced by Doxorubicin in Rat Heart
verfasst von
Meherzia Mokni
Sonia Hamlaoui-Guesmi
Mohamed Amri
Lamjed Marzouki
Ferid Limam
Ezzedine Aouani
Publikationsdatum
01.06.2012
Verlag
Humana Press Inc
Erschienen in
Cardiovascular Toxicology / Ausgabe 2/2012
Print ISSN: 1530-7905
Elektronische ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-012-9155-1

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