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Current Allergy and Asthma Reports
Erschienen in: Current Allergy and Asthma Reports 3/2015

01.03.2015 | Immunologic/Diagnostic Tests in Allergy (M Chapman and A Pomés, Section Editors)

Interfaces Between Allergen Structure and Diagnosis: Know Your Epitopes

verfasst von: Anna Pomés, Maksymilian Chruszcz, Alla Gustchina, Alexander Wlodawer

Erschienen in: Current Allergy and Asthma Reports | Ausgabe 3/2015

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Abstract

Allergy diagnosis is based on the patient's clinical history and can be strengthened by tests that confirm the origin of sensitization. In the past 25 years, these tests have evolved from the exclusive in vivo or in vitro use of allergen extracts, to complementary molecular-based diagnostics that rely on in vitro measurements of IgE reactivity to individual allergens. For this to occur, an increase in our understanding of the molecular structure of allergens, largely due to the development of technologies such as molecular cloning and expression of recombinant allergens, X-ray crystallography, or nuclear magnetic resonance (NMR), has been essential. New in vitro microarray or multiplex systems are now available to measure IgE against a selected panel of purified natural or recombinant allergens. The determination of the three-dimensional structure of allergens has facilitated detailed molecular studies, including the analysis of antigenic determinants for diagnostic purposes.
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Metadaten
Titel
Interfaces Between Allergen Structure and Diagnosis: Know Your Epitopes
verfasst von
Anna Pomés
Maksymilian Chruszcz
Alla Gustchina
Alexander Wlodawer
Publikationsdatum
01.03.2015
Verlag
Springer US
Erschienen in
Current Allergy and Asthma Reports / Ausgabe 3/2015
Print ISSN: 1529-7322
Elektronische ISSN: 1534-6315
DOI
https://doi.org/10.1007/s11882-014-0506-9

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