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17.04.2020 | Original Article

Photobiomodulation alters the viability of HUVECs cells

verfasst von: Stella Maris Lins Terena, Raquel Agnelli Mesquita-Ferrari, Andreia Martinelli de Siqueira Araújo, Kristianne Porta Santos Fernandes, Maria Helena Fernandes

Erschienen in: Lasers in Medical Science | Ausgabe 1/2021

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Abstract

The aim of the present study was to investigate the influence of low-level red (660 nm) and infrared (780 nm) laser with four different radiance exposures on human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs (1.5 × 10⁴) were incubated in 96-well culture plates. The cells were maintained in M199 medium supplemented with 20% fetal bovine serum, 1% antibiotic (penicillin), 1% anti-mycotic (Fungizone), and 1% endothelial cell growth supplement. After centrifugation, irradiations (660/780 nm, 40 mW, 1, 5, 10, and 20 J/cm², 1 s, 5 s, 10 s, and 20 s, respectively, total energy 0.4 J, 2 J, 4 J, and 8 J, and beam spot size at target 0.04 cm²) were performed at the bottom of Falcon tubes such that the laser beam directly reached the cell without passing through the culture medium. The cells were divided into groups based on radiant exposures. Cell viability and protein concentration were verified after 1, 2, 3, 6, 8, and 10 days. Red laser increased the cell viability and protein concentration in all groups (three-way ANOVA, p < 0.05) beginning on the second day. The greatest peak compared with the control was found when the radiant exposure was 5 J/cm² and 10 J/cm². Infrared laser inhibited cell viability and modulated the protein concentration in the cells, with the highest peak protein concentration found on the second day in the group with radiant exposure of 1 J/cm² and 10 J/cm² (three-way ANOVA, p < 0.05). Red laser increased the viability and concentration of total proteins in HUVECs, whereas infrared laser had an inhibitory effect on cell viability, while maintaining the total protein concentration similar to that found in the control group.

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Titel: Photobiomodulation alters the viability of HUVECs cells
verfasst von: Stella Maris Lins Terena
Raquel Agnelli Mesquita-Ferrari
Andreia Martinelli de Siqueira Araújo
Kristianne Porta Santos Fernandes
Maria Helena Fernandes
Publikationsdatum: 17.04.2020
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2021
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03016-z

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Photobiomodulation alters the viability of HUVECs cells

Abstract

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Abstract

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