Erschienen in:
01.03.2016 | Original Research Paper
A critical role of Dectin-1 in hypersensitivity pneumonitis
verfasst von:
Mari Higashino-Kameda, Toshiki Yabe-Wada, Shintaro Matsuba, Kazuya Takeda, Kazushi Anzawa, Takashi Mochizuki, Koichi Makimura, Shinobu Saijo, Yoichiro Iwakura, Hirohisa Toga, Akira Nakamura
Erschienen in:
Inflammation Research
|
Ausgabe 3/2016
Einloggen, um Zugang zu erhalten
Abstract
Objectives and design
Hypersensitivity pneumonitis (HP) is a pulmonary disease caused by repeated exposure to various aspiration antigens, including bacteria and fungi. Although TLRs are known to be required for the generation of HP triggered by bacteria, the significance of fungal receptors remains unclear. The present study aimed to investigate whether Dectin-1 and Dectin-2 contribute to the development of experimental HP triggered by the fungus Trichosporon asahii (T. asahii) that causes summer-type HP.
Materials and methods
We investigated the binding between Dectin-Fc protein and T. asahii by a dot blot assay. We performed the histological and flow cytometric analysis in the HP model using Dectin-1-deficient (Dectin-1−/−) and Dectin-2−/− mice. We also investigated Th17/Th1 responses in lung cells, and measured an IL-17-promoting cytokine IL-23 from bone marrow-derived dendritic cells (BMDCs) by ELISA.
Results
Dectin-1 bound more strongly to T. asahii than Dectin-2. Dectin-1−/− mice barely developed HP, whereas both wild-type mice and Dectin-2−/− mice developed similar lung diseases. Dectin-1 deficiency decreased the infiltration of neutrophils and monocyte-derived macrophages and repressed the expansion of lung CD4+IL-17A+ cells. The production of IL-23 p19 was reduced in Dectin-1−/− BMDCs.
Conclusions
These data suggested Dectin-1 plays a critical role in the development of fungus-induced HP.