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Forensic Science, Medicine and Pathology

Erschienen in:

11.10.2021 | Original Article

A novel approach for rapid cell assessment to estimate DNA recovery from human bone tissue

verfasst von: Thien Ngoc Le, Dzenita Muratovic, Oliva Handt, Julianne Henry, Adrian Linacre

Erschienen in: Forensic Science, Medicine and Pathology | Ausgabe 4/2021

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Abstract

We report on the use of a DNA staining dye to locate and record nucleated osteocytes and other bone-related cells within sections of archived formalin-fixed and paraffin-embedded human tibia from which informative DNA profiles were obtained. Eleven of these archived tibia samples were sectioned at a thickness of 5 µm. Diamond™ Nucleic Acid Dye was applied to the sections and cells within the matrix of the bone fluoresced so that their location and number of cells could be photographed. DNA was isolated from these 11 samples using a standard extraction process and the yields were quantified by real-time PCR. Complete STR profiles were generated from ten bone extracts where low-level inhibition was recorded with an incomplete STR profile obtained from one sample with higher inhibition. The stained image of this sample showed that few cells were present. There was a significant relationship between the number of DD-stained cells and the number of alleles obtained (p < 0.05). Staining cells to determine the prevalence of bone cell nuclei allows a triage of samples prior to any subsequent DNA profiling.

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Titel: A novel approach for rapid cell assessment to estimate DNA recovery from human bone tissue
verfasst von: Thien Ngoc Le
Dzenita Muratovic
Oliva Handt
Julianne Henry
Adrian Linacre
Publikationsdatum: 11.10.2021
Verlag: Springer US
Erschienen in: Forensic Science, Medicine and Pathology / Ausgabe 4/2021
Print ISSN: 1547-769X
Elektronische ISSN: 1556-2891
DOI: https://doi.org/10.1007/s12024-021-00428-3

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

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A novel approach for rapid cell assessment to estimate DNA recovery from human bone tissue

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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