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01.01.2016 | Original Research Paper

A novel N-acetyl-glucosamine lectin of Lonchocarpus araripensis attenuates acute cellular inflammation in mice

verfasst von: Alana F. Pires, Natália V. F. C. Rodrigues, Pedro Marcos G. Soares, Ronaldo de Albuquerque Ribeiro, Karoline S. Aragão, Márcia M. Marinho, Mayara T. L. da Silva, Benildo S. Cavada, Ana Maria S. Assreuy

Erschienen in: Inflammation Research | Ausgabe 1/2016

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Abstract

Objective and design

This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis.

Material/methods

LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hemagglutinating activity against rabbit erythrocytes. In vivo LAL was assessed for the anti-inflammatory activity via intravenous injection (i.v.) in Swiss mice (25–30 g; n = 6/group) in models of paw edema and peritonitis. Statistical analysis: ANOVA (p < 0.05).

Results

LAL revealed two bands of 30 and 60 kDa (SDS-PAGE) and exhibited hemagglutinating activity. LAL (10 mg/kg) inhibited the paw edema (77 %) and vascular permeability (26 %) induced by carrageenan, and the paw edema induced by serotonin (80 %), bradykinin (49 %), sodium nitroprusside (83 %), TNF-α (75 %) and PGE₂ (64 %). LAL also inhibited the neutrophil migration induced by fMLP (70 %) or carrageenan (69 %). The intravital microscopy showed that LAL inhibited rolling (83 %) and adhesion (70 %) of leukocytes. LAL anti-inflammatory effect was reversed by its association with N-acetyl-glucosamine. The nine-daily treatment with LAL (10 mg/kg; i.v.) showed no toxicity.

Conclusion

The novel N-acetyl-d-glucosamine-binding lectin isolated from L. araripensis seeds presents anti-inflammatory effect involving the lectin domain and the inhibition of 5-HT, BK, PGE₂, NO, TNF-α and leukocyte rolling and adhesion.

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Titel: A novel N-acetyl-glucosamine lectin of Lonchocarpus araripensis attenuates acute cellular inflammation in mice
verfasst von: Alana F. Pires
Natália V. F. C. Rodrigues
Pedro Marcos G. Soares
Ronaldo de Albuquerque Ribeiro
Karoline S. Aragão
Márcia M. Marinho
Mayara T. L. da Silva
Benildo S. Cavada
Ana Maria S. Assreuy
Publikationsdatum: 01.01.2016
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 1/2016
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-015-0889-7

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Objective and design

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