Introduction
IgA nephropathy (IgAN) was first discovered and described by Berger and Hinglais in 1968 [
1]. Clinical manifestations are microscopic hematuria, or gross hematuria, and proteinuria. Kidney pathology shows the deposition of IgA immune complexes, leading to glomerulonephritis characterized by mesangial cells and stromal hyperplasia. IgAN is one of the most common types of primary glomerulonephritis in China, accounting for about 45% of primary glomerulonephritis. IgAN can occur at any age and is common in children and adolescents [
2,
3]. About 5–40 years after diagnosis, about 20% -40% progress to kidney failure [
4].
The pathological classification of IgAN includes Lee's classification, Hass classification, and Oxford classification, which are important guidelines for the treatment and prediction of IgAN [
5]. Lee’s classification considers histologic lesions that may predict the prognosis of IgAN, and is easy to operate and apply [
6]. Oxford Classification Working Group through univariate analysis proved that mesangial proliferation, segmental glomerulosclerosis, and tubular atrophy/interstitial fibrosis are independent high-risk factors for the progression of IgAN [
7]. So far, the pathogenesis of IgAN is still unclear, but the immune pathogenesis of IgAN has been widely studied and reported [
8,
9]. Galactose deficiency-IgA1 (Gd-IgA1) complex deposits in the mesangium region [
10], and the mesangial cells proliferation are the common pathological changes in IgAN [
11]. In addition, damaged mesangial cells release pro-inflammatory factors, such as IL-6, MCP-1, TNF-α and so on, which promotes the inflammatory response, and further aggravates kidney damage [
12].
Chemokine (C–C motif) receptor 2 (CCR2) is a G protein-coupled receptor, a protein of 355 amino acid residues, which is mainly expressed in leukocytes, endothelial cells, macrophages, and smooth muscle cells, etc. [
13]. Chemokines and their receptors are involved in important physiological and pathological processes, such as inflammation and autoimmune diseases, transplant rejection, tumor growth and metastasis [
14,
15]. Previous report has shown an association between monocyte chemoattractant protein-1 (MCP-1) and CCR2 gene polymorphisms and higher grade histopathology of IgAN by Lee's classification [
16]. Animal experiment results show that CCR2 autoimmune-deficient mice have significantly reduced lupus nephritis [
17]. In addition, many previous studies showed that the blockade of chemokine (C–C motif) ligand 2 (MCP-1) and its receptor CCR2 signaling pathway reduced inflammatory cytokines, such as IL-6 and TNF-α [
18,
19]. However, so far the role of CCR2 in the pathology and inflammation of IgAN is unknown. This study investigated the expression of CCR2 in kidney tissues of children with IgAN, and its correlation with Lee's grade and the expression levels of inflammatory factors in kidney tissues.
Materials and methods
Reagents
Rabbit anti-CCR2 antibody was obtained from BioVision (Milpitas, CA). Rabbit anti-MCP-1, rabbit anti-IL-17, mouse anti-IL-6 and rabbit anti-TNF-α antibodies and were obtained from AbCam (Cambridge, MA). Pre-immune rabbit serum, secondary antibodies (PV-9000 kit) and DAB Detection Kit were purchased from Zsbio (Beijing, CHN). FITC-labeled anti-human IgA was purchased from Zsbio (Beijing, CHN). Other chemicals and reagents were purchased from Zsbio (Beijing, CHN).
Clinical specimen collection
Fifteen children with a diagnosis of IgAN presenting to the Department of Pediatrics, First Affiliated Hospital of Anhui Medical University, were enrolled between July 2014 to September 2017. All children were diagnosed with primary IgAN by kidney biopsy [
20]. The procedure for the kidney biopsy was performed as previously described with some modifications [
21], and the pathological staining was shown in the supplementary materials (sFigure
1 and sFigure
2). All patients did not receive the treatment with steroid or other immunosuppressant agents before kidney biopsy. All children were excluded from secondary IgAN caused by allergic purpura nephritis, lupus nephritis, or hepatitis B virus-related nephritis. Eight control kidney tissue specimens were taken from children with kidney surgery because of other kidney diseases, such as kidney duplication. Wax block specimens of kidney biopsy were used for subsequent immunohistochemical staining. Twelve control blood and urine specimens were taken from normal children with health checkup. Study protocol was approved by the hospital institutional review board of the First Affiliated Hospital of Anhui Medical University, and the number of the ethics approval was NO. 20140235. Kidney biopsy specimens from patients and control group had obtained the informed consent of the patient's family.
Immunohistochemistry
The wax block of kidney biopsy tissue and control kidney tissues were cut at 5 μm thickness sections. The procedure for immunohistochemistry has been previously described [
22]. Briefly, the sections were treated with conventional dewaxing tissue sections to water, and sodium citrate buffer solution antigen repair, and 3% H
2O
2 to reduce the non-specific staining, and 5% BSA was added to block endogenous antigen. The slides were incubated with rabbit anti-CCR2 (dilution 1:200), or free-immune serum (1:50), rabbit anti-MCP1 (1:100), IL-17 (1:100), TNF-ɑ (1:100) and mouse anti-IL-6 (1:500) overnight at 4 °C. After rinsing in PBS, the sections were incubated with peroxidase-conjugated goat anti-rabbit or anti-mouse IgG at room temperature for 1 h. After PBS washing for three times, DAB color solution dropped onto the section, and incubated for 3–5 min. Then the section was stained with hematoxylin for 2 min. After drying and transparent, the section was observed under microscope. The analysis of specific staining was performed using Image J software (NIH, Bethesda, MD, USA). Negative controls were performed by replacing the primary antibody with free-immune serum, and the results was shown in the supplementary materials (sFigure
3). The relative optical density (OD) was counted at 200 × magnification, and the positive signaling were counted in twenty randomly selected sections using the software of Image J v1.8.0 (National Institutes of Health, Bethesda, MD).
Blood and urine testing
The serum and urine samples were obtained by centrifugation, and 20ul was used to measure urine red blood cell count (URBC), 24-h urine protein (UPro), blood urea nitrogen (BUN), and serum creatinine (SCr) were measured on Abbot architect ci8200 analyser (Abbott, Abbot Park, IL, USA). Each sample was tested 3 times, and the average value was used for statistical analysis.
Statistical analysis
Graph Pad Prism 5 software was used to plot and process the experimental data. All data were presented as the means ± SEM. Differences between two groups were analyzed by Student's t test for unpaired data. ANOVA followed by Scheffé’s post-doc test was used in multi-group analysis. We performed Spearman’s correlation tests to specify relationships among the variables. P values of < 0.05 were considered significant.
Discussion
IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis worldwide, which may occur at any age, and is most common in children and adolescents [
2,
3]. IgAN is a frequent cause of kidney failure, and 20–40% of IgAN patients eventually progress to kidney failure within 20 years of onset [
23,
24]. At present, the immune pathogenesis of IgAN has been widely studied and reported, but the specific pathogenesis is still unclear [
8,
9].
The role of chemokines in the pathogenesis of IgAN has gradually attracted attention [
17,
25]. Chemokine (C–C motif) receptor 2 (CCR2) is a G protein-coupled receptor with a 355 amino acid residue protein, which is mainly expressed in leukocytes, endothelial cells, macrophages and smooth muscle cells [
26]. CCR2 is the receptor of MCP-1 (monocyte chemoattractic protein 1), and play an important role in inflammatory disorders [
16,
27]. MCP-1/CCR2 signaling was involved in human crescentic glomerulonephrtitis and murine lupus nephritis [
28,
29]. Previous reports have shown that MCP-1 and CCR2 gene polymorphisms may affect the progression of IgAN [
16]. Previous study has shown that CCR2 activation plays an important role in the development of hypertensive nephropathy via increased oxidative stress and inflammation [
30]. Several reports have shown that glomerulosclerosis and tubulointerstitial fibrosis were significantly ameliorated in CCR2-/- mice with adriamycin nephropathy, and accompanying with the reduction of macrophage and fibrocyte infiltration and inflammation in glomeruli and the tubulointerstitium [
31‐
33]. These evidences indicate that CCR2 mediate inflammation, and cause collagen accumulation, and promote renal fibrosis, and ultimately cause severe kidney damage [
32]. However, the role of CCR2 in IgAN is not yet clear.
In this study, we found that CCR2 positive signals were only expressed in the glomeruli of children with IgAN, and importantly most of the CCR2 positive signals were expressed in glomerular mesangial cells. Interestingly, we found that CCR2 expression was positively correlated with Lee’s grade in IgAN children, and CCR2 positive signal in children with Lee’s pathological grade III to IV was significantly higher than that of Lee’s grade I to II (
P < 0.01). Although our study only included 15 children with IgAN, the exciting thing is that we found that CCR2 expression had a significant positive correlation with Lee’s grade (
P = 0.0001) (Fig.
2C). We hypothesize that Gd-IgA deposition stimulates mesangial cells, and up-regulates CCR2 expression. Elevated CCR2 could increase the expression of multiple cytokines and chemokines, such as MCP-1, IL-6 and TNF alpha, and induces mesangial cell proliferation and matrix deposition, and further aggravate kidney damage [
16,
34]. Therefore, CCR2 may play an important regulatory role in the pathological process and inflammatory response of children IgAN.
It has been reported that histological features of IgAN on diagnostic biopsy are different in children and adults [
35]. Children IgAN are more likely to have mild histologic lesions and less advanced chronic lesions, such as glomerulosclerosis and interstitialfibrosis, compared to adults [
36‐
38]. Therefore, previous physicians have long believed that children IgAN is a benign disease to remission, and occasionally to late relapse during adulthood [
39,
40]. However, recent statistics show that the prognosis of children with IgAN is as serious as that of adults [
39,
41,
42]. Recent studies show that children IgAN presents with more acute mesangial proliferation and inflammation, while in adults it correlates with chronic lesions and nephron reduction [
39]. Proteinuria in children with IgAN appears to be due to acute mesangial proliferation and inflammation [
35].
In our study, 24 h Upro is significantly increased in children IgAN (
P < 0.01), and the expression of chemokines and cytoinflammatory factors in the kidney tissue of children with IgAN increased significantly, such as MCP-1, TNF alpha, IL-6 and IL-17, which are similar to previous studies [
12,
43]. Interestingly, we found that the expression levels of inflammatory factors in children with Lee’s pathological grade III to IV was significantly higher than that of Lee’s grade I to II (
P < 0.01). These results indicate that chemokines and cytoinflammatory factors play an important role in the pathological changes of IgAN.
Chemokines and cytoinflammatory factors are assumed to play an important role as mediators of inflammation and as progression factors in various kidney disorders [
44‐
46]. Various cytokines, such as IL-6 and TNF-α, have been increased in the kidney tissues of patients with IgAN, which contributes to further glomerular injury [
46,
47]. Previous study has shown that MCP-1 can activate the NF-κB pathway by binding to CCR2 to promote the secretion of inflammatory factor IL-6 [
48]. Similarly, MCP-1 can also stimulate vascular endothelial cells to produce IL-6 through this pathway, and promote the development of glomerulonephritis [
49]. Interleukin 17 (IL-17) plays an important role in the pathogenesis of autoimmune diseases [
50]. Recently, the role of Th17 cells producing proinflammatory interleukin 17A (IL-17A) was established in the pathogenesis of various glomerulonephritis [
51]. Therefore, IL-17A is considered as a potential biomarker of IgA nephropathy [
52,
53]. In our study, MCP-1, TNF alpha, IL-6 and IL-17 in the kidney tissue of children with IgAN increased significantly. Importantly, we found that CCR2 signal was positively correlated with the expression levels of inflammatory factors. The results indicate that CCR2 signal may play an important role in the inflammatory response of IgAN.
In summary, we report that CCR2 expression is significantly increased in glomerular mesangial cells of IgAN children, especially in IgAN patients with Lee’s grade III to IV. Importantly, CCR2 expression is positively correlated with Lee’s grade and the expression levels of inflammatory factors in IgAN children. These results suggesting that CCR2 signal is associated with the pathological grade and the inflammatory response in children IgA nephropathy, indicating that CCR2 plays a pathogenic role in IgAN, and targeting CCR2-associated signaling pathways may be a strategy for the treatment of IgAN. However, further mechanistic studies are needed to fully explore the roles of CCR2 in IgAN.
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