Clinical characteristics and prognoses in pediatric neuroblastoma with bone or liver metastasis: data from the SEER 2010–2019

Neuroblastoma is one of the most common malignant solid tumors in children that starts from the neural crest [1], accounting for around 15% of pediatric cancer-related deaths [2, 3]. Approximately 70% of patients with neuroblastoma exhibit metastasis [4, 5], more than half can experience distant metastasis at diagnosis [6]. Although the overall prognosis of neuroblastoma patients is good, patients with metastasis usually have poor survival even after radical treatment [7, 8]. The 5-year survival of high-risk children is lower than 50% [9].

Metastasis acts as an independent risk factor for survival in neuroblastoma, and bone and liver are the most common single metastatic sites of this disease [10]. Evidence demonstrated that two-thirds of neuroblastoma patients with bone metastasis had a primary site in the adrenal gland, with a 5-year survival rate of 62.1%, and age and tumor size were important factors affecting patients’ survival [11]. In addition, liver metastasis accounts for greater than 20% of neuroblastoma metastasis [12], but there is a lack of research on the clinical characteristics and prognosis of neuroblastoma patients with liver metastasis. Further studies are needed to investigate the differences in clinical characteristics, prognoses, and prognostic factors among neuroblastoma patients with different metastatic sites. Besides, treatment methods for the primary site (such as surgery or radiotherapy) exerted significant influences on the survival of neuroblastoma patients [13, 14]. Chemotherapy was shown to improve survival in patients suffering from neuroblastoma [15]. However, no relevant studies have been performed to explore the impacts of different therapeutic methods on the survival of neuroblastoma patients with different metastatic sites.

This study intended to probe into differences in clinical characteristics and prognoses of neuroblastoma patients with different metastatic sites (bone metastasis alone, liver metastasis alone, and both bone and liver metastasis), and assess the impacts of treatment methods on their survival, based on the Surveillance, Epidemiology, and End Results (SEER) database.

To the best of our knowledge, this study was the first to evaluate differences in clinical characteristics and prognoses among neuroblastoma patients with bone metastasis alone, liver metastasis alone, and both bone and liver metastasis, and the influences of different therapeutic methods on survival. The results demonstrated significant differences in age, tumor size, surgery for the primary site, chemotherapy, radiation, brain metastasis, lung metastasis, and vital status between patients with liver metastasis alone, bone metastasis alone, and both liver and bone metastasis. In contrast to patients with liver metastasis alone, patients with bone metastasis alone or both had significantly poorer 3- and 5-year CSS. Besides, for patients with bone metastasis, those receiving chemotherapy had significantly better 3-year CSS than those without chemotherapy. For patients with liver metastasis, receiving radiation was associated with significantly worse 3-year CSS. These findings may assist in understanding the disease features (including survival) of metastatic neuroblastoma and the role of treatments in patients with metastasis, which might further facilitate timely interventions to get favorable prognoses.

He et al. [11] investigated the clinical characteristics, survival and prognostic factors of neuroblastoma patients with bone metastasis. Another study by Liu et al. [10] explored the metastasis pattern of neuroblastoma, overall survival and CSS of neuroblastoma patients with different metastatic sites, and risk factors for metastasis. The current study focused on neuroblastoma patients with bone or liver metastasis, since bone and liver metastasis accounted for relatively high proportions, and assessed clinical features, 3- and 5-year CSS, and therapeutic approaches among patients with bone and liver metastasis. Patients with bone metastasis alone and both liver and bone metastasis tended to be older than ≥ 3 years, have a tumor size of ≥ 5 cm, receive surgery for the primary site, chemotherapy, and radiation, and have brain metastasis, compared with those with liver metastasis alone. Clinicians could pay attention to these characteristics, and provide early counseling and management measures for people at risk of metastasizing to different sites. It was demonstrated that age over 1 year and tumors of 5–10 cm were correlated with an increased risk of bone metastasis [10]. A previous study proposed that age, tumor biology and survival were associated with the metastasis pattern of neuroblastoma. The biological characteristics of the tumor change with age, leading to differences in the metastasis ability or tumor affinity with specific sites [16]. Monte et al. [17] also revealed that chemotherapy and/or radiotherapy changed the metastasis pattern of neuroblastic tumors. A previous study reported that neuroblastoma patients with bone metastasis had a CSS of 64.1% [18]. We found more information on CSS of patients with different metastatic sites: the 3-year CSS rate of patients with liver metastasis alone, bone metastasis alone, and both liver and bone metastasis was 83.87%, 71.97%, and 62.16%, separately; the 5-year CSS rate of patients with liver metastasis alone, bone metastasis alone, and both liver and bone metastasis was 75.00%, 55.16%, and 45.28%, respectively. Evidence from larger sample sizes is necessitated for CSS rate corroboration. Further, compared with liver metastasis alone, bone metastasis alone or both were associated with significantly worse 3- and 5-year CSS, and patients with bone metastasis (combined with liver metastasis or not) exhibited poorer CSS, according to this study. A possible explanation for the better 3- and 5-year CSS in the liver metastasis group is the presence of more 4S stage neuroblastoma in the liver metastasis group. In patients without brain metastasis, the association between bone or liver metastasis and 3- and 5-year CSS was consistent with the above, while for patients without lung metastasis, merely both liver and bone metastasis was associated with significantly worse 5-year CSS than liver metastasis alone. This may be attributed to the relatively small sample size for our analysis. Future large-scale investigations should be conducted to validate these findings. Liu et al. [10] illustrated similar CSS in the bone metastasis alone group, liver metastasis alone group, and the both bone and liver metastasis group, but covariables were not taken into consideration in their research. We have controlled for potential confounding factors in this analysis to minimize their effect.

As regards the role of different treatments in survival of neuroblastoma patients, this study showed that for patients with bone metastasis, those with chemotherapy had better 3-year CSS than those without, and for patients with liver metastasis, receiving radiation was associated with worse 3-year CSS, suggesting that chemotherapy may confer survival benefit in neuroblastoma patients with bone metastasis. Induction chemotherapy (IC) can shrink the tumor, and lower the risk of further metastasis in neuroblastoma [18, 19]. Increased dose intensity in chemotherapy was related to greater response and survival of neuroblastoma patients [20]. More research is required to verify the protective effect of chemotherapy on survival in metastatic neuroblastoma. Concerning the unfavorable impact of radiotherapy in patients with liver metastasis, radiation treatment may bring late side effects for individuals with neuroblastoma, such as hypothyroidism, lung and heart abnormalities, musculoskeletal abnormalities, and growth and developmental failure [21, 22]. Another possible explanation is that radiation can elevate the risk of secondary neoplasms, because vesicles secreted from irradiated neuroblastoma cells promote proliferation and invasion related to the epithelial-to-mesenchymal transition in non-irradiated cells [23], which may be associated with worse survival in 3 years. Besides, complications after radiotherapy included hypertension, veno-occlusive disease, nerve lesion, and bowel obstructions [21]. Patients with neuroblastoma undergoing radiation may be at a higher risk of vascular injury from the tumor and surgery, since they were chosen to receive radiotherapy for the more invasive, surgically challenging tumors [24], posing a threat to survival. A prior review showed that palliative radiation contributed to high response rates and symptomatic relief, whereas survival is unsatisfactory in metastatic neuroblastoma [25]. Notably, the sample size used to analyze the role of radiotherapy in patients with liver metastasis is relatively small in this study. At present, the relationship between radiotherapy and CSS among neuroblastoma patients with bone or liver metastasis is under-researched, which necessitates large-scale studies in the future. As to surgery for neuroblastoma, it remains an important component in treating high-risk neuroblastoma and controlling the localized tumor [14]. Kubota [26] put forward that the influence of surgery varies by different clinical situations, and the benefits of surgery to survival in high-risk neuroblastoma may be limited. Neuroblastoma with macroscopic residual tumor died within 18 months after surgery due to systemic metastasis [27], and surgical eradication may be crucial [28]. We found no significant association between surgery for the primary site and surgery for other regional or distant sites and CSS in neuroblastoma patients with bone metastasis or liver metastasis. A small sample size may be an explanation. For another, survival advantages brought by surgery may be offset by adverse effects from a great incidence of surgical complications and the level of resection.

Using this nationally representative data, differences in clinical features and CSS and impacts of treatments on CSS among neuroblastoma patients with bone or liver metastasis were exhibited. Close attention should be paid to patients with bone metastasis, and early interventions should be taken when necessary. Adjustment of therapeutic methods such as radiotherapy may improve outcomes in neuroblastoma patients with bone or liver metastasis. Some limitations should be noted. First, this study had a retrospective study, which may introduce selection bias. Second, the treatment protocol of NB mainly included chemotherapy, radiotherapy, and surgery, and more detailed treatments were not available from the SEER database. Besides, since the SEER database did not provide all the information required for neuroblastoma risk stratification by Children’s Oncology Group (COG) and SIOP, such as International Neuroblastoma Staging System (INSS) stage, MYCN status and DNA ploidy, the risk of patients with neuroblastoma could not be determined in this study. Third, information on different metastatic sites was collected only after 2010 in the SEER, and the data used in this study came from the SEER 2010–2019, which may result in insufficient follow-up time. Ultimately, the findings of this study may not be generalizable to populations in other countries.

Compared with liver metastasis alone, bone metastasis alone or both was associated with poorer 3- and 5-year CSS. For patients with bone metastasis, those with chemotherapy had better 3-year CSS than those without. For patients with liver metastasis, receiving radiation was associated with worse 3-year CSS. More studies are warranted to support these findings.