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Inflammation Research
Erschienen in: Inflammation Research 5/2015

01.05.2015 | Original Research Paper

Different profiles of notch signaling in cigarette smoke-induced pulmonary emphysema and bleomycin-induced pulmonary fibrosis

verfasst von: Shi Li, Xiaofei Hu, Zheng Wang, Meng Wu, Jinnong Zhang

Erschienen in: Inflammation Research | Ausgabe 5/2015

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Abstract

Objective

Different profiles of Notch signaling mediate naive T cell differentiation which might be involved in pulmonary emphysema and fibrosis.

Methods

C57BL/6 mice were randomized into cigarette smoke (CS) exposure, bleomycin (BLM) exposure, and two separate groups of control for sham exposure to CS or BLM. The paratracheal lymph nodes of the animals were analyzed by real-time PCR and immunohistochemistry. Morphometry of the lung parenchyma, measurement of the cytokines, and cytometry of the bronchoalveolar lavage fluid (BALF) were also done accordingly.

Results

In comparison with controls, all Notch receptors and ligands were upregulated by chronic CS exposure, especially Notch3 and DLL1 (P < 0.01), and this was in line with emphysema-like morphology and Th1-biased inflammation. While Notch3 and DLL1 were downregulated by BLM exposure (P < 0.01), those was in line with fibrotic lung remodeling and Th2 polarization.

Conclusions

This founding implies that the CS exposure but not the BLM exposure is capable of initiating Notch signaling in lymphoid tissue of the lung, which is likely relevant to the pathogenesis of pulmonary emphysema. Unable to initiate the Th1 response or inhibit it may lead to Th2 polarization and aberrant repair.
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Metadaten
Titel
Different profiles of notch signaling in cigarette smoke-induced pulmonary emphysema and bleomycin-induced pulmonary fibrosis
verfasst von
Shi Li
Xiaofei Hu
Zheng Wang
Meng Wu
Jinnong Zhang
Publikationsdatum
01.05.2015
Verlag
Springer Basel
Erschienen in
Inflammation Research / Ausgabe 5/2015
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-015-0816-y

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