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Cardiovascular Toxicology

Erschienen in:

01.09.2010

Epigallocatechin-3-Gallate Protects Na⁺ Channels in Rat Ventricular Myocytes Against Sulfite

verfasst von: Haiying Wei, Ziqiang Meng

Erschienen in: Cardiovascular Toxicology | Ausgabe 3/2010

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Abstract

Sulfite (bisulfite/sulfite) can affect voltage-gated sodium (Na⁺) channels (VGSC) in a concentration-dependent manner in isolated rat ventricular myocytes. In this study, the effect of epigallocatechin-3-gallate (EGCG) on VGSC in isolated ventricular myocytes was studied. Ventricular myocytes were exposed to 10 μM bisulfite/sulfite for 10 min, and EGCG was then administered in different concentrations (10, 30, 50 μg ml⁻¹). Decreased activity of superoxide dismutase, catalase (CAT) and glutathione peroxidase (GPx) was observed after bisulfite/sulfite exposure, with significant increase in Na⁺ currents (I _Na) and alterations in half-activation voltage and half-inactivation voltage. Intracellular reactive oxygen species (ROS) such as hydrogen peroxide (H₂O₂), hydroxyl (OH^·), and superoxide anion (O ₂ ^·− ) were increased. After EGCG treatment, activity of the aforementioned enzymes increased while the ROS level decreased. The effects progressed with increasing amounts of EGCG, up to a level similar to blank control at the dose of 50 μg ml⁻¹ EGCG, EGCG also reduced the I _Na and reversed the alterations in half-activation voltage and half-inactivation voltage. In conclusion, EGCG could protect Na⁺ channels in rat ventricular myocytes against the oxidative damage induced by sulfite as a scavenger of the ROS.

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Titel: Epigallocatechin-3-Gallate Protects Na+ Channels in Rat Ventricular Myocytes Against Sulfite
verfasst von: Haiying Wei
Ziqiang Meng
Publikationsdatum: 01.09.2010
Verlag: Humana Press Inc
Erschienen in: Cardiovascular Toxicology / Ausgabe 3/2010
Print ISSN: 1530-7905
Elektronische ISSN: 1559-0259
DOI: https://doi.org/10.1007/s12012-010-9075-x

Springer Medizin

Abstract

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