Introduction
Biogenesis, composition, and function of exosomes
Role of exosome in the regulation of inflammation
Exosome derived from | Disease | Mechanism | Pathway | Effect | References |
---|---|---|---|---|---|
Monocytes | Tumor cell | Exosomes derived from monocytes secrete various inflammatory cytokines, including IL6, IL-1β, IL-8, and TNF-α | STAT3 and NFκB | Pro-inflammatory effects | [51] |
Macrophages | Breast and stomach tumor | Exosomes derived from macrophages in breast and stomach tumors stimulate the production of inflammatory cytokines, including GCSF, IL-6, IL-8, IL1β, CCL2, and TNF-α | NF-κB | Pro-inflammatory effects | [52] |
Dendritic cells | Tumor cell | Induction of IL6 and Secretion of TGFβ | STAT3 | Pro-inflammatory effects | |
Myeloid-derived suppressor cells (MDSC) | Tumor cell | Induction of the proinflammatory cytokine Cox 2 and an increase in inflammatory cytokines such as IL-6, TNF-α, VEGF, CCL2 | STAT3 | Pro-inflammatory effects | [54] |
Natural killer cells | Tumor | Inhibition of NK cell activation mediated by IL-2 | By inducing Smad phosphorylation, it can disrupt cytotoxicity and reduce NKG2D receptor expression | Anti-inflammatory effects | [55] |
Regulatory T cells (Treg) | Tumor | Expansion phosphorylation of relevant transcription factors IL-10 and TGF- β1 | immunosuppression | Anti-inflammatory effects | [56] |
Macrophages | _ | Decreased expression of IL-6 | Decreased expression of IL-6 It can also act as a negative regulator in the JAK/STAT pathway | Anti-inflammatory effects |
Regulation of exosome biogenesis by viral proteins
Virus regulates exosome production
Virus regulates exosome composition
Regulation of inflammation by respiratory viruses through exosomes
Respiratory syncytial viruses
Influenza virus
Parainfluenza virus
Rhinoviruses
Coronaviruses
Adenoviruses
Conclusion and outlook
Virus | Exosome source | Exosome cargo | Sample (in vitro, in vivo) | Induction or inhibition of inflammation | Note | References |
---|---|---|---|---|---|---|
Respiratory syncytial viruses | RSV-infected A549 cells | – | In vitro (RSV-infected A549 cells) | Induction | Exosomes derived from RSV-infected A549 cells secrete significantly higher levels of RANTES, IP-10, and TNF-α, which activate the innate immune response and may through the release of pro-inflammatory cytokines and apoptosis in receptor cells have antiviral effects | [101] |
Influenza | Respiratory epithelial cell lines | NP, NS1, M1, HA | In vitro (Respiratory epithelial cell lines) | Inhibition | Exosomes derived from respiratory epithelial cell lines infected with influenza virus showed an anti-viral response, which may inhibit inflammation in the early stages | [106] |
Influenza | Bronchial alveolar lavage fluid (BALf) of influenza virus | – | In vitro (bronchial alveolar lavage fluid (BALf) of influenza virus) | Induction | Exosomes derived from bronchial alveolar lavage fluid (BALf) of influenza virus produce IL-6, MCP-1, and TNF, which are inflammatory cytokines and increase inflammation | [106] |
Influenza | Macrophages, fibroblasts, T cell, and B cell lines | Nucleoprotein (NP) and non-structural protein (NS1) | In vitro (macrophages, fibroblasts, T cell, and B cell lines) | Induction | Levels of pro-inflammatory cytokines, such as IFN-γ, IL-1β, and CXCL8, were also elevated by the exosomes | [136] |
Influenza | Bronchoalveolar lavage fluid (BALF) | miR-483-3p | In vitro (bronchoalveolar lavage fluid (BALF)) | Induction | The transfer of miR-483-3p from bronchoalveolar lavage fluid (BALF) caused the expression of type I interferon and proinflammatory cytokine genes and strengthened their face | [137] |
Influenza | Exosomes derived from H5N1-infected chickens | Viral proteins, NP and NS1 | In vitro (exosomes derived from H5N1-infected chickens) | Induction | The presence of viral proteins such as NP and NS1 in exosomes derived from H5N1-infected chickens increases the level of pro-inflammatory cytokines such as IFN-γ, IL-1β, and IL-8 | [119] |
Influenza | Bronchoalveolar lavage fluid (BALF) | miR-483-3p, miR-374c-5p, miR-466i-5p, miR-203-3p | In vitro (bronchoalveolar lavage fluid (BALF)) | Induction | miR-483-3p, miR-374c-5p, miR-466i-5p, miR-203-3p in exosomes derived from bronchoalveolar lavage fluid (BALF) significantly increased IFN-β expression, proinflammatory cytokine gene expression, and upregulates interferon-stimulated genes (ISGs), including IL6, CCL2, TNF-α, and SP110 | [138] |
Parainfluenza | Madin–Darby bovine kidney (MDBK) cells inoculated with CPIV3 | miRNA 11 | In vitro (Madin–Darby bovine kidney (MDBK) cells inoculated with CPIV3) | Unknown | These exosomes could transfer CPIV3 genetic materials to recipient cells to establish a productive infection and promote viral replication | [139] |
Rhinoviruses | Primary bone marrow-derived DCs (BMDCs) | MiR155 | in vitro and in vivo | Induction | miR-155 in exosomes derived from primary bone marrow-derived DCs (BMDCs) is essential for Th2-mediated eosinophilic inflammation in the lung and induces inflammatory conditions in the body | [118] |
Rhinoviruses | RV-infected AECs | – | In vitro | Induction | Exosomes from RV-infected AECs yielded significant inflammatory cytokines/chemokines such as CXCL8, which induced an inflammatory state in the body | [140] |
Rhinoviruses | Bronchoalveolar lavage fluid | – | In vitro | Induction | – | [141] |
Coronaviruses | Lung macrophages | Nsp12 and Nsp13 | In vitro | Induction | Nsp12 and Nsp13 in exosomes derived from lung macrophages lead to the activation of nuclear factor κB (NF-κB) and subsequent induction of an array of inflammatory cytokines | [127] |
Coronaviruses | Patient plasma | Tenascin-C (TNC) and fibrinogen-β (FGB) | In vitro | Induction | Tenascin-C (TNC) and fibrinogen-β (FGB) induce the production of pro-inflammatory cytokines through interaction with the NF-κB inflammatory signaling pathway. FGB is transported via plasma exosomes and potentially induces pro-inflammatory cytokine signals in distant organ cells | [128] |
Coronaviruses | A549 cell | Viral protein E, Nsp7, Nsp10, Nsp12, Nsp13, and slight protein M | In vitro | Induction | The RNA polymerase, Nsp12, alone inhibits tumor necrosis factor (TNF)-α and interleukin (IL)-6. Furthermore, a synergistic effect of Nsp12 working with Nsp13 was observed where, compared to Nsp12 alone, there was a significant induction of TNF-α, IL-1β, and IL-6, which are inflammatory cytokines. In contrast, M protein, Nsp13 alone, or Nsp10 did not | [127] |
Coronaviruses | Endothelial cells | – | In vitro | Induction | Exosomes derived from endothelial cells infected with coronaviruses lead to NLRP3 inflammasome activation in endothelial cells of distant organs, which ultimately leads to IL-1β secretion and inflammatory response | [128] |
Adenoviruses | Bone marrow-derived dendritic cells (DCs) | Expressing viral IL-10 | In vitro | Inhibition | [142] | |
Adenoviruses | Derived from FasL-expressing DC | – | In vitro | Inhibition | Mice are immunized with a specific antigen, keyhole limpet hemocyanin (KLH). Then a Th1-mediated inflammatory response is induced 10 to 14 days after immunization by injection of the particular antigen into the hind pads. Mice immunized with KLH 106 DC or 1 μg exosome received in one hind paw 12 h before KLH booster injection into both hind paws. Delivery of DC/FasL or DC/FasL-derived exosomes significantly reduced paw swelling not only in the treated paw but also in the contralateral paw. Untreated suppressed 24, 48, and 72 h after antigen injection. These results show that genetically modified DC-expressing FasL as well as DC/FasL-derived exosomes are equally effective in suppressing the DTH response not only in the treated paw, but they are also in the untreated opposite paw | [135] |
Adenovirus | Infected A549 | – | In vitro | Induction | A549-derived exosomes infected with adenoviruses significantly increased IL-1β protein, which induced inflammatory conditions in the body | [134] |