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Erschienen in:
01.04.2011 | Original Research Paper
Expression analysis of the early chemokine response 4 h after in vitro traumatic brain injury
Erschienen in: Inflammation Research | Ausgabe 4/2011
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Objective and design
The importance of cytokine- and chemokine-mediated neuroinflammation in the progress of brain injury is becoming increasingly evident. We investigated the early local cytokine and chemokine expression and the development of tissue injury after moderate mechanical hippocampus trauma.
Material or subjects
Mouse organotypic hippocampal slice cultures.
Treatment
Drop-weight trauma in the CA1 region of the hippocampus.
Methods
Staining of necrotic tissue, PCR array and evaluation, real-time PCR, statistical analysis with a two-tailed, independent t test.
Results
At 12 and 24 h after trauma, the tissue injury spread from the primary mechanical lesion to the entire hippocampal formation. A pronounced up-regulation of distinct chemokine transcripts was found 4 h after in vitro traumatic brain injury which preceded the development of the secondary injury.
Conclusions
The enhanced expression of inflammatory genes might contribute to the development of the secondary trauma and could pinpoint future neuroinflammatory and neuroprotective targets for research and treatment.