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01.12.2023 | Original Article

Frequency and function of circulating regulatory T-cells in biliary atresia

verfasst von: Satoru Oita, Takeshi Saito, Akemi Sakamoto, Lisa Fujimura, Yukiko Ohara, Takashi Fumita, Keita Terui, Mitsuyuki Nakata, Shugo Komatsu, Gen Matsuura, Masahiko Hatano, Tomoro Hishiki

Erschienen in: Pediatric Surgery International | Ausgabe 1/2023

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Abstract

Purpose

Although the impairment of regulatory T-cells (Tregs) has been shown in the liver or portal area of biliary atresia (BA) the frequency and function of circulating Tregs in BA patients is poorly understood. We aimed to investigate the frequency and function of circulating Tregs in BA patients.

Methods

Peripheral blood mononuclear cells were collected from 25 BA patients and 24 controls. Treg frequency was measured by flow cytometry; function was determined by T-cell proliferation assay. We also assessed the association between Treg frequency/function and clinical parameters in BA cases.

Results

There was no significant difference between the two groups in both frequency (BA: 3.4%; control: 3.2%; p = 0.97) and function (BA: 22.0%; control: 7.5%; p = 0.23) of Tregs. We further focused on 13 preoperative BA patients and 14 age-matched controls. Neither Treg frequency nor function were significantly different (frequency: BA: 4.6%; control: 3.4%; p = 0.38, function: BA: 2.7%; control: 7.6%; p = 0.89). There was no association between Treg frequency/function and clinical parameters.

Conclusion

Neither the frequency nor function of circulating Tregs was affected in BA patients, suggesting the negative role of circulating Tregs in the pathogenesis of BA. Further investigation of local Treg profiles is warranted.

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Titel: Frequency and function of circulating regulatory T-cells in biliary atresia
verfasst von: Satoru Oita
Takeshi Saito
Akemi Sakamoto
Lisa Fujimura
Yukiko Ohara
Takashi Fumita
Keita Terui
Mitsuyuki Nakata
Shugo Komatsu
Gen Matsuura
Masahiko Hatano
Tomoro Hishiki
Publikationsdatum: 01.12.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 1/2023
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-022-05307-8

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Frequency and function of circulating regulatory T-cells in biliary atresia