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Erschienen in:
21.05.2019 | Short Communication
HMGB1 decreases CCR-2 expression and migration of M2 macrophages under hypoxia
Erschienen in: Inflammation Research | Ausgabe 8/2019
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Objective
The hypoxic milieu at tumor microenvironment is able to drive the behavior of infiltrating tumor cells. Considering that hypoxia-mediated HMGB1 release is known to promote tumor growth, as well to enhance the pro-tumoral profile of M2 macrophages by a RAGE-dependent mechanism, it is tempting to evaluate the potential contribution of HMGB1 under hypoxia to restrain M2 macrophages mobility.
Methods
CCR-2 expression was evaluated in M2 polarized macrophages by western blotting and immunocytochemistry. The secreted levels of CCL-2 and the migration capability were evaluated using an ELISA and a chemotaxis assay, respectively.
Results
HMGB1, under hypoxic conditions, markedly reduce both the production of CCL-2 and the expression of its receptor CCR-2; and reduced the migration capacity of M2 macrophages.
Conclusions
These results provided new insights into the mechanisms that regulate M2 macrophages mobility at the tumor microenvironment.