Introduction
The sclerocorneal limbus, the transition area between the corneal and conjunctival epithelium of the ocular surface, is the niche of the limbal epithelial stem cells (LESC). The specific niche site is the basal epithelium of the palisades of Vogt, formed by limbal crypts and ridges. It is responsible for the adequate homeostasis and continuous regeneration of the corneal epithelium. The limbus also acts as a physical barrier that prevents the migration of conjunctival cells and blood vessels onto the cornea [
1‐
3].
Damage or dysfunction of LESC or the niche induces limbal stem cell deficiency (LSCD), which prevents the normal regeneration of the corneal epithelium. This clinical alteration can be triggered by different diseases such as congenital aniridia, severe ocular surface inflammation, chemical burns, or multiple surgeries, among others [
4,
5]. LSCD causes recurrent epithelial defects, corneal ulcerations, neovascularization, conjunctivalization, and squamous metaplasia [
6,
7]. Consequently, the LSCD patient usually suffers from photophobia, decreased vision, inflammation, blepharospasm, and pain [
4,
5,
8,
9].
Up to now, many different approaches have been used to treat LSCD [
10,
11]. Among them, cultivated limbal epithelial transplantation (CLET) from an autologous or allogeneic small limbal biopsy is the most common stem cell-based treatment. This therapy provides a source of stem cells to regenerate the normal corneal epithelium resulting in clinical improvements [
12‐
14]. In this regard, our research group has wide experience improving the techniques for cultivating LESC [
15‐
19], and clinical management of LSCD, including CLET [
13,
20].
In the last decades, the use of mesenchymal stem cells (MSC) for regenerative medicine has grown significantly due to their beneficial properties. MSC have immunosuppressive functions by inhibiting proliferation, differentiation, and chemotaxis of B cells in inflamed tissues, where MSC can migrate [
21,
22]. MSC also have non-immunogenic characteristics allowing them to perform allogeneic transplantation without immunosuppression [
23]. Therefore, the benefits of MSC for the treatment of ocular surface alterations have been extensively studied in experimental models [
24‐
27]. Moreover, our research group performed the first clinical trial in human eyes as a proof-of-concept demonstrating that MSC transplantation (MSCT) can be as safe and effective as CLET for LSCD management [
28].
In vivo confocal microscopy (IVCM) is a non-invasive technique that allows high-resolution imaging of the cornea; thus, it has become an appropriate tool for studying corneal anomalies [
29]. The first report of this clinical trial showed that eyes undergoing MSCT obtained similar improvement in the epithelial phenotype of the central cornea than CLET ones [
28]. However, no previous study has reported if MSCT can also have a positive effect on limbal niche structures in LSCD eyes. Consequently, the objective of this sub-analysis from a phase I–II clinical trial was to assess the effect of MSCT and CLET on the limbal anatomical structures of LSCD eyes as measured with IVCM technique.
Methods
The protocol of the clinical trial was approved by the University Hospital Ethics Committee and the Spanish Agency for Medicine and Medical Devices (AEMPS). This study followed the principles of the Declaration of Helsinki, the Good Manufacturing and Clinical Practice guidelines, and the European Union Tissues and Cells Directive. All patients signed the written informed consent after the nature of the study was explained.
The present study is a sub-analysis of a proof-of-concept phase I–II randomized, controlled, and double-masked clinical trial (EudraCT 2010-023535-42; ClinicalTrials.gov Identifier: NCT01562002). This sub-analysis was performed because changes in the limbal structures of LSCD eyes after CLET or MSCT were not included as a primary or secondary outcome measure of the clinical trial. The specific details pertaining to the clinical trial design, patient characteristics, randomization and masking, procedures and primary evaluation endpoints have been already widely detailed [
28]. Briefly, recruited patients had unilateral or bilateral, severe and/or total corneal epithelial failure due to LSCD, with blindness or low vision due to central corneal opacification. Allogeneic bone marrow-derived MSC and limbal epithelial cells isolated from a cadaveric limbal biopsy were cultured on amniotic membrane and later transplanted to the ocular surface. The cell dose in MSCT was around 250,000 cells per graft. All scheduled transplants were randomly allocated to CLET or MSCT.
In Vivo Confocal Microscopy of the Limbal Niche
The Heidelberg Retinal Tomograph version 3 (HRT3) with a Rostock cornea module (RCM) (RCM-HRT3) (Heidelberg GmbH, Heidelberg, Germany) was used to analyze the corneal and limbal areas before and after transplantation. Disposable and sterile contact caps (TomoCap
® Heidelberg GmbH) were used to allow contact between the cornea and the objective lens of the IVCM. A lubricating gel (Viscotears
® 0.2% with Carbomero 980; Novartis, Basel, Switzerland) was used to cover and fill the corneal contact caps. Also, topical anesthesia (tetracaine 0.1% and oxybuprocaine 0.4%; Alcon Cusí, Barcelona, Spain) was used prior to IVCM image acquisition. High-contrast digital images with a field of view of 400 × 400 μm (0.16 mm
2) were acquired. The transverse resolution and thickness of the optical sections were 2 and 4 μm, respectively. Sequential scans of the central cornea and superior, inferior, temporal, and nasal limbus (12, 3, 6, 9 o’clock positions) using the manual frame acquisition technique were obtained following methods previously described [
13,
20]. A minimum of 40 z-scan images were visualized at each examination point from the epithelium to the stroma in the central cornea, or to the palisades of Vogt in the limbus. To increase the likelihood of assessing the same location, external fixation targets were presented to the contralateral eye—one distance target for the central cornea and four eccentric ones for each quadrant of the limbus. Central cornea scans were performed to assess the phenotype of the central epithelium. Cell morphology was classified as corneal, conjunctival, or mixed-type depending on the IVCM findings [
30,
31]. When classifying the central phenotype, corneal epithelium was considered as a multi-layered anatomical structure having polygonal and flat cells with hyperreflective nuclei in the superficial layer, progressively decreasing in size in the intermediate layers, and small cells without detectable nuclei with reflective borders in the basal layer [
32]; and the conjunctival epithelium as a stratified anatomical structure of cuboidal or polygonal cells having hyporeflective cytoplasm with or without detectable nuclei, and barely defined borders [
30,
33]. Limbal scans were performed to assess the presence or absence of palisades of Vogt and the morphologic transition of epithelial cells from corneal to conjunctival phenotype. The palisades of Vogt were defined as radial hyperreflective stromal ridges that follow a branching or interconnecting pattern alternated with columns of limbal basal epithelium (rete pegs) [
30,
34,
35].
Central cornea epithelial phenotype and limbal IVCM images were acquired before and 12 months after MSCT and CLET therapies. The IVCM images were obtained and later analyzed, always by the same experienced researcher (I.P.S.) using the Heidelberg Eye Explorer software (V. 1.5.10.0. 2006; Heidelberg GmbH) to avoid interobserver variability. The main outcomes of the IVCM analysis were the epithelial phenotype of the central cornea (corneal, conjunctival, or mixed), and the presence or absence of transition zones and palisades of Vogt in the four quadrants of the limbus.
Statistical Analysis
Statistical analysis was performed using R statistical package version 4.0.0. Quantitative characteristics were expressed as mean ± standard deviation (SD), and qualitative variables were described as numbers and percentages. McNemar's test was used to assess changes in the dichotomous variables before and 12 months after transplantation surgery, while Wilcoxon test was used for ordinal variables. To assess if there were any relationships between the changes in the epithelial phenotype of the central cornea after surgery and the changes in the palisades of Vogt and transition zones of the limbus, Mann–Whitney test was used.
Discussion
Our research group performed a pilot clinical trial to assess if MSCT therapy was as safe and efficient as CLET to treat LSCD eyes. A healthier corneal epithelium was achieved in 85.7 and 77.8% of MSCT and CLET cases, respectively [
28]. However, histological and anatomical changes in the limbus were not considered as primary or secondary endpoints of the study, thus they were not reported. IVCM technique allows us to obtain minimally invasive in vivo images of the corneal epithelium and the limbal area, which can be evaluated in situ to better evaluate the success or failure of the treatments [
33,
36,
37]. There are several publications analyzing the limbal niche by IVCM after surgical techniques, including CLET [
20,
33,
36,
38], however, no report exists addressing the limbal changes after MSCT. Therefore, the present sub-analysis from a phase I–II clinical trial was carried out to assess if limbal areas in LSCD eyes could also show some histological regeneration after MSCT therapy.
The IVCM images of the central cornea obtained in the present clinical trial showed an improvement in the central corneal epithelial phenotype 12 months after both stem cell type transplantations. These results are like the ones reported by Pedrotti et al. [
33], who observed that 46.1% of 13 LSCD eyes exhibited corneal phenotype and 30.8% had a mixed phenotype 1 year after autologous CLET surgery. Likewise, in the study of Mastropascua et al. [
36], including six LSCD eyes after 12 months of autologous CLET, a corneal phenotype was observed in 50% of eyes and a mixed phenotype in 33.3% of the cases.
In the limbus of the healthy human cornea, a progressive transition of epithelial cells from corneal to conjunctival epithelial phenotype can be observed by IVCM [
34]. However, this regular epithelial transition is usually altered in LSCD eyes [
30]. Consequently, in the present sub-analysis of the clinical trial, we assessed if the detection of the transition zone was higher after transplantation because it can be considered suggestive of restoration of limbal function. Twelve months after surgery, the improvement in the transition zones was solely significant for the nasal quadrant considering the total sample. Although the sample size of the present clinical trial is higher than other studies assessing transplantation in LSCD eyes [
33,
36], when we analyzed the two different transplantation groups, the statistical power was reduced for each group, and it might have played a role in the absence of statistically significant changes after surgery for each group. Pedrotti et al. [
33] have also observed similar findings to ours. They reported a higher number of transition zones in LSCD eyes (
n = 13) after CLET. Specifically, in 46.1% of the cases, the transition zone was fully present and in 30.8% cases, it was partially present 1 year after surgery. Mastropasqua et al. [
36] have also assessed changes in the superficial morphology of the limbus after CLET, reporting that all six of their cases showed an abrupt transition from corneal to conjunctival epithelial phenotype.
The detection of palisades of Vogt in LSCD eyes is important not only because it is an indicator of a healthy histology but also because they play an essential role in maintaining the normal state and function of LESC [
3]. In the present clinical trial, after surgery, only two more LSCD eyes showed palisades of Vogt in at least one quadrant. This minor change was only significant for the inferior quadrant considering the whole sample. Previous authors have also assessed the presence of palisades of Vogt after transplantation surgery. Both Pedrotti et al. [
33] and Mastropasqua et al. [
36] have reported the absence of recovery of palisades of Vogt after CLET.
The outcomes of the present clinical trial and those previously reported [
33,
36,
38] showed slight histological changes in the limbus after transplantation surgery when assessing the transition from conjunctival to corneal epithelial phenotype. However, major histological improvements in the limbus, like the appearance of palisades of Vogt after surgery, might be much more difficult to observe. In the present study, a healthier epithelial phenotype in the central cornea, in addition to a higher number of transition zones in the limbus, was observed. However, the relationship between both improvements was not significant. Previous authors [
33] have shown that it is possible to observe improvements in the epithelial phenotype of the central cornea without detecting palisades of Vogt in the limbus after transplantation. Thus, improvements in the corneal epithelial phenotype can be detected in the absence of a normal limbal stem cell niche. In fact, it has been previously hypothesized that if stem cells are located after surgery in different positions within the cornea (e.g., basal layers of corneal epithelium), they might be able to sustain the corneal surface cell mass [
33].
The differences in the recovery of the limbal architecture after transplantation among different studies could be related to several factors, including the etiology and severity of the LSCD eyes included in each study, or the number of the transplanted cells (250,000 in the present clinical trial) and even the administration route. Most of the eyes included in the present clinical trial and other studies suffered from LSCD due to chemical burns. However, the severity of the ocular injury usually depends on the contact surface area and the degree of penetration of the alkaline or acid. Thus, outcomes after transplantation are very likely to vary depending on the LSCD severity before surgery. Besides, once the safety and efficacy of MSCT have been already demonstrated by our research group [
28], further clinical trials must be conducted using alternative routes for cell administration, such as subconjunctival injections, which can increase the number of cells administered [
26].
In this sub-analysis study, some changes were observed in the limbus 1 year after transplantation. However, the secretion of different paracrine factors by the transplanted cells could also be implicated in the regeneration of the central corneal epithelium. Corneal wound healing depends on the presence of growth factor, such us epidermal growth factor (EGF), whose topical administration has been demonstrated to improve the corneal epithelial wound closure, keratinocyte growth factor, hepatocyte growth factor, and transforming growth factor (TGF), among others [
39]. In this regard, both LESC and MSC secrete paracrine growth factors, which might have contributed to the corneal epithelium regeneration. In addition, limbal epithelial cells secrete different factors like the mediator of immunological system regulation TGF-α and β, tumor necrosis factor (TNF), fibroblast growth factor (FGF), and leukemia inhibitory factor [
40]. Besides, several significant factors are also secreted by MSC, such as EGF, pigment epithelium-derived factor (PEDF), TGF-β, and the tumor necrosis factor-inducible gene 6 protein, which are implicated in neuropathic pain inhibition, present anti-inflammatory properties, stimulate limbal stem cell colony-forming efficiency, and prevent neovascularization [
41,
42].
The present sub-analysis from a phase I–II clinical trial has some limitations. First, although the assessment of the limbus in LSCD eyes using IVCM technique is very useful to image the cornea and limbus, it is still a semi-automatic diagnostic technique that relies on the ability and experience of the clinician to obtain high-quality images, and the assessment is still subjective and very difficult in advanced LSCD eyes due to light scattering [
33]. Moreover, the technique requires contact, which can cause discomfort to the patient, and increases the difficulty of image acquisition. In fact, in the present clinical trial, it was not possible to obtain IVCM images in any of the four limbal quadrants in two LSCD eyes, despite successful central corneal assessment. In addition, in some LSCD eyes, it was not possible to obtain high-quality images in all limbal quadrants. Thus, although we observed a minor change in the detection of palisades of Vogt in the inferior quadrant after transplantation, detection of palisades in any quadrant might be missed during baseline assessment, because adequate detection is much more difficult in LSCD eyes than in normal ones or healthier corneas (i.e., after transplantation surgery). Second, the sample size of this study can be considered adequate in comparison with previous studies assessing the outcomes of transplantation surgeries in LSCD. However, when the MSCT and CLET groups were independently analyzed, the statistical power was reduced, and the change previously observed in the limbal structures lost its statistical significance. Fortunately, it was possible to recruit enough LSCD eyes to show that MSCT provided similar histological changes in the central cornea and limbus as eyes undergoing CLET.