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Lasers in Medical Science

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16.03.2020 | Original Article

Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla

verfasst von: Yaoyao Yang, Tingting Zhu, Yan Wu, Chunxia Shu, Qiang Chen, Juan Yang, Xiang Luo, Yao Wang

Erschienen in: Lasers in Medical Science | Ausgabe 9/2020

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Abstract

This study aimed to evaluate the effects of low-energy blue LED irradiation on the osteogenic differentiation of stem cells from the apical papilla (SCAPs). SCAPs were derived from human tooth root tips and were irradiated with 0 (control group), 1 J/cm², 2 J/cm², 3 J/cm², or 4 J/cm² blue light in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP), alizarin red staining, and real-time polymerase chain reaction (RT-PCR). The results of the MTT assay indicated that SCAPs in the LED groups exhibited a lower proliferation rate than those in the control group, and there were statistically differences between the 2 J/cm², 3 J/cm², and 4 J/cm² groups and the control group (P < 0.05). The results of the ALP and alizarin red analyses showed that blue LED promoted osteogenic differentiation of the SCAPs. And 4 J/cm² blue light upregulates the expression levels of the osteogenic/dentinogenic genes ALP, dentin sialophosphoprotein (DSPP), dentin matrix protein-1 (DMP-1), and osteocalcin (OCN) in SCAPs. Our results confirmed that low-energy blue LED at 1 J/cm², 2 J/cm², 3 J/cm², and 4 J/cm² could inhibit the proliferation of SCAPs and promotes osteogenic differentiation of SCAPs. Further in vitro studies are required to explore the mechanisms of the effects by low-energy blue LED.

Dixin C, Hongyu L, Mian W et al (2018) The origin and identification of mesenchymal stem cells in teeth: from odontogenic to non-odontogenic. Current Stem Cell Research & Therapy 13(1):39–45

Sonoyama W, Liu Y, Fang D et al (2006) Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS One 1:e79

Nada OA, El BRM (2018) Stem cells from the apical papilla (SCAP) as a tool for endogenous tissue regeneration. Frontiers in Bioengineering and Biotechnology 6:103

Huang TJ, Sonoyama W, Liu Y et al (2008) The hidden treasure in apical papilla: the potential role in pulp/dentin regeneration and bioroot engineering. J Endod 34(6):645–651

Shiehzadeh V, Aghmasheh F, Shiehzadeh F et al (2014) Healing of large periapical lesions following delivery of dental stem cells with an injectable scaffold: new method and three case reports. Indian J Dent Res 25(2):248

Bakopoulou A, Leyhausen G, Volk J et al (2011) Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP). Arch Oral Biol 56(7):709–721

Pereira MC, de Pinho CB, Medrado AR et al (2010) Influence of 670 nm low-level laser therapy on mast cells and vascular response of cutaneous injuries. J Photochem Photobiol B Biol 98(3):188–192

Basso FG, Oliveira CF, Kurachi C et al (2013) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci 28(2):367–374

Pasternak-Mnich K, Ziemba B, Szwed A et al (2019) Effect of photobiomodulation therapy on the increase of viability and proliferation of human mesenchymal stem cells. Lasers Surg Med

10.

Kim JE, Woo YJ, Sohn KM et al (2017) Wnt/β-catenin and ERK pathway activation: a possible mechanism of photobiomodulation therapy with light-emitting diodes that regulate the proliferation of human outer root sheath cells. Lasers Surg 49(10):940–947

11.

Oliveira FA, Matos AA, Santesso MR et al (2016) Low intensity lasers differently induce primary human osteoblast proliferation and differentiation. J Photochem Photobiol B 163:14–21

12.

Konig CJ, Buhner M, Murling G (2010) Blue-light irradiation regulates proliferation and differentiation in human skin cells. J Investig Dermatol 130(1):259

13.

Arnolda G, Chien TD, Hayen A et al (2018) A comparison of the effectiveness of three LED phototherapy machines, single- and double-sided, for treating neonatal jaundice in a low resource setting. PLoS One 13(10):e0205432

14.

Higuchi A, Shen PY, Zhao JK et al (2011) Osteoblast differentiation of amniotic fluid-derived stem cells irradiated with visible light. Tissue Eng A 17(21–22):2593–2602

15.

Ginani F, Soares DM, Alexandre DORH et al (2018) Low-level laser irradiation induces in vitro proliferation of stem cells from human exfoliated deciduous teeth. Lasers Med Sci 33(1):95–102

16.

Diao S, Lin X, Wang L et al (2017) Analysis of gene expression profiles between apical papilla tissues, stem cells from apical papilla and cell sheet to identify the key modulators in MSCs niche. Cell Prolif 50(3):e12337

17.

Pagin MT, de Oliveira FA, Oliveira RC et al (2014) Laser and light-emitting diode effects on pre- osteoblast growth and differentiation. Lasers Med Sci 29(1):55–59

18.

Zhu T, Wu Y, Zhou X et al (2019) Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro. Lasers Med Sci 34(7):1473–1481

19.

Huang TJ, Gronthos S, Shi S (2009) Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res 88(9):792–806

20.

Chueh LH, Huang TJ (2006) Immature teeth with periradicular periodontitis or abscess undergoing apexogenesis: a paradigm shift. J Endod 32(12):1205–1213

21.

Estrela C, Alencar AH, Kitten GT et al (2011) Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration. Braz Dent J 22(2):91–98

22.

Diogenes A, Hargreaves KM (2017) Microbial modulation of stem cells and future directions in regenerative endodontics. J Endod 43(9):S95–S101

23.

Chrepa V, Pitcher B, Henry MA et al (2017) Survival of the apical papilla and its resident stem cells in a case of advanced pulpal necrosis and apical periodontitis. J Endod 43(4):561–567

24.

Lin LM, Kim SG, Martin G et al (2018) Continued root maturation despite persistent apical periodontitis of immature permanent teeth after failed regenerative endodontic therapy. Australian Endodontic Journal the Journal of the Australian Society of Endodontology Inc 44(3):292–299

25.

Huang GTJ, Yamaza T, Shea LD et al (2010) Stem/progenitor cell–mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Eng A 16(2):605–615

26.

Na S, Zhang H, Huang F et al (2013) Regeneration of dental pulp/dentine complex with a three-dimensional and scaffold-free stem-cell sheet-derived pellet. J Tissue Eng Regen Med 10(3):261–270

27.

Yan M, Wang L, Lei G et al (2013) Proliferation and osteo/odontoblastic differentiation of stem cells from dental apical papilla in mineralization-inducing medium containing additional KH2PO4. Cell Prolif 46(2):214–222

28.

Marques, Márcia Martins, Diniz, Ivan a Márcia Alves et al (2016) Photobiomodulation of dental derived mesenchymal stem cells: a systematic review. Photomed Laser Surg 34(11):500–508

29.

Lipovsky A, Nitzan Y, Gedanken A, Lubart R (2010) Visible light-induced killing of bacteria as a function of wavelength: implication for wound healing. Lasers Surg Med 42(6):467–472

30.

Alghamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27(1):237–249

31.

Barboza CAG, Ginani F, Soares DM et al (2014) Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells. Einstein (Sao Paulo) 12(1):75–81

32.

Soares D M , Ginani F , Henriques, ág uida Gomes et al (2015) Effects of laser therapy on the proliferation of human periodontal ligament stem cells. Lasers Med Sci 30(3):1171–1174

33.

Mvula B, Mathope T, Moore T et al (2008) The effect of low level laser irradiation on adult human adipose derived stem cells. Lasers Med Sci 23(3):277–282

34.

Zaccara IM, Ginani F, Mota-Filho HG et al (2015) Effect of low-level laser irradiation on proliferation and viability of human dental stem cells. Lasers Med Sci 30(9):2259–2264

35.

Whelan HT, Smits RL, Buchmann EV et al (2001) Effect of NASA light-emitting diode irradiation on wound healing. J Clin Laser Med Surg 19(6):305–314

36.

Posten W, Wrone DA, Dover JS et al (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31(3):334–340

37.

Owen TA, Aronow M, Shalhoub V et al (1990) Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143(3):420–430

38.

Turrioni APS, Basso FG, Montoro LA et al (2014) Phototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth. J Dent 42(10):1292–1299

39.

Li WT, Leu YC (2007) Effects of low level red-light irradiation on the proliferation of mesenchymal stem cells derived from rat bone marrow. Conf Proc IEEE Eng Med Biol Soc 2007:5830–5833

40.

Soleimani M, Abbasnia E, Fathi M et al (2012) The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts—an in vitro study. Lasers Med Sci 27(2):423–430

41.

Ballini A, Mastrangelo F, Gastaldi G et al (2015) Osteogenic differentiation and gene expression of dental pulp stem cells under low-level laser irradiation: a good promise for tissue engineering. J Biol Regul Homeost Agents 29(4):813–822

42.

Borzabadi-Farahani A (2016) Effect of low-level laser irradiation on proliferation of human dental mesenchymal stem cells; a systemic review. J Photochem Photobiol B Biol 162:577–582

43.

Li R, Peng L, Ren L et al (2009) Hepatocyte growth factor exerts promoting functions on murine dental papilla cells. J Endod 35(3):382–388

44.

Feng JQ, Huang H, Lu Y et al (2003) The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. J Dent Res 82(10):776–780

45.

Wang S, Mu J, Fan Z et al (2012) Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla. Stem Cell Res 8(3):346–356

Titel: Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla
verfasst von: Yaoyao Yang
Tingting Zhu
Yan Wu
Chunxia Shu
Qiang Chen
Juan Yang
Xiang Luo
Yao Wang
Publikationsdatum: 16.03.2020
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-02995-3

Springer Medizin

Abstract

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