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Journal of the Association for Research in Otolaryngology

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15.02.2024 | Original Article

Measuring Optokinetic Reflex and Vestibulo-Ocular Reflex in Unilateral Vestibular Organ Damage Model of Zebrafish

verfasst von: Kang Hyeon Lim, Hong Ki Kim, Saemi Park, Eunjung Han, Insik Song, Hee Soo Yoon, Jaeyoung Kim, Yunkyoung Lee, Yong Hun Jang, Yoon Chan Rah, Sang Hyun Lee, June Choi

Erschienen in: Journal of the Association for Research in Otolaryngology | Ausgabe 2/2024

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Abstract

One-sided vestibular disorders are common in clinical practice; however, their models have not been fully established. We investigated the effect of unilateral or bilateral deficits in the vestibular organs on the vestibulo-ocular reflex (VOR) and optokinetic reflex (OKR) of zebrafish using in-house equipment. For physical dislodgement of the otoliths in the utricles of zebrafish larvae, one or both utricles were separated from the surrounding tissue using glass capillaries. The video data from VOR and OKR tests with the larvae was collected and processed using digital signal processing techniques such as fast Fourier transform and low-pass filters. The results showed that unilateral and bilateral damage to the vestibular system significantly reduced VOR and OKR. In contrast, no significant difference was observed between unilateral and bilateral damage. This study confirmed that VOR and OKR were significantly reduced in zebrafish with unilateral and bilateral vestibular damage. Follow-up studies on unilateral vestibular disorders can be conducted using this tool.

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Titel: Measuring Optokinetic Reflex and Vestibulo-Ocular Reflex in Unilateral Vestibular Organ Damage Model of Zebrafish
verfasst von: Kang Hyeon Lim
Hong Ki Kim
Saemi Park
Eunjung Han
Insik Song
Hee Soo Yoon
Jaeyoung Kim
Yunkyoung Lee
Yong Hun Jang
Yoon Chan Rah
Sang Hyun Lee
June Choi
Publikationsdatum: 15.02.2024
Verlag: Springer US
Erschienen in: Journal of the Association for Research in Otolaryngology / Ausgabe 2/2024
Print ISSN: 1525-3961
Elektronische ISSN: 1438-7573
DOI: https://doi.org/10.1007/s10162-024-00936-3

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Measuring Optokinetic Reflex and Vestibulo-Ocular Reflex in Unilateral Vestibular Organ Damage Model of Zebrafish

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