05.01.2024 | Original Article
Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique
A randomized split-mouth study
verfasst von:
Raquel Fernanda Bachiega Morelli, Paulo Nelson-Filho, Karla Carpio Horta, Magda Feres, José Tarcísio Lima Ferreira, Fábio Lourenço Romano, Maria Bernadete Sasso Stuani, Maria Conceição Pereira Saraiva, Caio Luiz Bitencourt Reis, Mirian Aiko Nakane Matsumoto
Erschienen in:
Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie
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Sonderheft 2/2024
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Abstract
Objective
The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures.
Materials and methods
Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa, FL, USA], and Ease [Obscure, 3M Unitek, Monrovia, CA, USA]) were randomly assigned to permanent canines of 25 patients (aged 11–18 years) undergoing corrective orthodontic treatment. After 30 days, the ligatures were removed, processed, and the biofilm composition was analyzed by checkerboard DNA–DNA hybridization for 40 bacterial species. The microbiological data were analyzed using a nonparametric mixed model.
Results
The ligatures presented intense microbial contamination after 30 days, but no statistically significant differences were observed among the three groups (p > 0.05). The levels of the evaluated individual species and proportions of the microbial complexes showed no statistically significant differences among the ligature groups (p > 0.05).
Conclusions
Esthetic elastomeric ligatures became multicolonized by several bacterial species after 30 days of exposure to the oral cavity. However, no relevant differences were observed among the biofilm composition formed on the different ligature brands.